Citation: Junghwa Lee, Seungah Lee, Seong Ho Kang. Wavelength-dependent three-dimensional single-molecule superlocalization imaging for yoctomole detection of thyroid-stimulating hormone on a quantum dot nanobiosensor[J]. Chinese Chemical Letters, ;2023, 34(12): 108383. doi: 10.1016/j.cclet.2023.108383 shu

Wavelength-dependent three-dimensional single-molecule superlocalization imaging for yoctomole detection of thyroid-stimulating hormone on a quantum dot nanobiosensor

Junghwa Lee ^{a, 1} ,
Seungah Lee ^{b, 1} ,
Seong Ho Kang ^{a, b, *,}

a.
Department of Chemistry, Graduate School, Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Republic of Korea
b.
Department of Applied Chemistry and Institute of Natural Sciences, Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Republic of Korea

shkang@khu.ac.kr

Received Date: 12 January 2023
Revised Date: 10 February 2023
Accepted Date: 27 March 2023
Available Online: 30 March 2023

Figures(3)

A wavelength-dependent three-dimensional (3D) superlocalization imaging method on gold nanoislands (GNIs) chip was developed as a supersensitive single-molecule thyroid-stimulating hormone (TSH) nanobiosensor. Scattered and fluorescent signals from gold nanoislands on the substrate and quantum dots (QDs) nanoprobes were simultaneously isolated and acquired within an evanescent field layer generated by total internal reflection (TIR) of incident light using a dual-view device. The 3D TIR fluorescence images of TSH-bound QDs on the GNIs were obtained using z-axis optical sectioning at 10 nm intervals before/after immunoreaction to identify the optimal conditions for detection. The localized centroid position of QD nanoprobes and GNI were distinguished at a subdiffraction limit resolution using 3D Gaussian fitting to the point spread function. The QD TSH nanobiosensor using wavelength-dependent 3D TIR fluorescence-based single-molecule localization microscopy (3D TIRF-SLM) imaging technique showed an excellent detection limit of 90 yoctomoles (~54 molecules) and a wide linear dynamic range of 1.14 zmol/L − 100 pmol/L for TSH. The detection sensitivity was about 4.4 × 10⁹ times higher than conventional enzyme-linked immunosorbent assay and could successfully quantify TSH in human serum. The wavelength-dependent 3D TIRF-SLM technique may emerge as a reliable platform for ultrahigh-sensitive nanobiosensors at the single-molecule level and early diagnosis with quantification of disease-related ultra-trace biomolecules.
- Single-molecule nanobiosensor,
- Thyroid stimulating hormone,
- Yoctomole detection,
- 3D superlocalization,
- Imaging
1. [1]
  N. Akkilic, S. Geschwindner, F. Höök, Biosens. Bioelectron. 151 (2020) 111944. doi: 10.1016/j.bios.2019.111944
2. [2]
  Y. Liu, B. Li, B. Liu, K. Zhang, Biosens. Basel 12 (2022) 1105. doi: 10.3390/bios12121105
3. [3]
  S. Lee, J. Lee, Y. Cao, C. An, S.H. Kang, Biosens. Bioelectron. : X 11 (2022) 100191.
4. [4]
  S.O. Kelley, ACS Sens. 2 (2017) 193–197. doi: 10.1021/acssensors.6b00691
5. [5]
  F. Karim, T.B. Smith, C. Zhao, J. Nanophotonics 12 (2017) 012504. doi: 10.1117/1.JNP.12.012504
6. [6]
  A. Shivanandan, H. Deschout, M. Scarselli, A. Radenovic, FEBS Lett. 588 (2014) 3595–3602. doi: 10.1016/j.febslet.2014.06.014
7. [7]
  D. Lando, U. Endesfelder, H. Berger, et al., Open Biol. 2 (2012) 120078. doi: 10.1098/rsob.120078
8. [8]
  J. Gunzenhäuser, N. Olivier, T. Pengo, S. Manley, Nano Lett. 12 (2012) 4705–4710. doi: 10.1021/nl3021076
9. [9]
  J. Rossy, D.M. Owen, D.J. Williamson, Z. Yang, K. Gaus, Nat. Immunol. 14 (2013) 82–89. doi: 10.1038/ni.2488
10. [10]
  A. Szymborska, A. de Marco, N. Daigle, et al., Science 341 (2013) 655–658. doi: 10.1126/science.1240672
11. [11]
  S. Ju, S. Lee, S.K. Chakkarapani, et al., Anal. Chem. 90 (2018) 5100–5107. doi: 10.1021/acs.analchem.7b05107
12. [12]
  S.K. Chakkarapani, Y. Sun, S.H. Kang, Sens. Actuator. B: Chem. 284 (2019) 81–90. doi: 10.1016/j.snb.2018.12.118
13. [13]
  S. Lee, J. Lee, I. Batjikh, H. Yu, S.H. Kang, ACS Sens. 7 (2022) 1372–1380. doi: 10.1021/acssensors.1c02572
14. [14]
  S. Ahn, P. Zhang, H. Yu, S. Lee, S.H. Kang, Anal. Chem. 88 (2016) 11070–11076. doi: 10.1021/acs.analchem.6b03069
15. [15]
  S. Ju, S. Lee, S.K. Chakkarapani, et al., Anal. Chem. 90 (2018) 5100–5107. doi: 10.1021/acs.analchem.7b05107
16. [16]
  Y. Cui, H. Chen, L. Hou, et al., Anal. Chim. Acta 738 (2012) 76–84. doi: 10.1016/j.aca.2012.06.013
17. [17]
  J. Lee, S. Lee, G. Lee, S.H. Kang, Biosens. Bioelectron. 220 (2023) 114894. doi: 10.1016/j.bios.2022.114894
18. [18]
  S. Lee, S.H. Kang, Talanta 99 (2012) 1030–1034. doi: 10.1016/j.talanta.2012.08.002
19. [19]
  S. Choi, J. Hwang, S. Lee, et al., Sens. Actuator. B: Chem. 240 (2017) 358–364. doi: 10.1016/j.snb.2016.08.178
20. [20]
  W. Jung, J. Han, J. Kai, et al., Lab Chip 13 (2013) 4653–4662. doi: 10.1039/c3lc50403d
21. [21]
  C. Shim, R. Chong, J.H. Lee, Talanta 171 (2017) 229–235. doi: 10.1016/j.talanta.2017.05.007
22. [22]
  S.L. Znoyko, A.V. Orlov, V.A. Bragina, M.P. Nikitin, P.I. Nikitin, Talanta 216 (2020) 120961. doi: 10.1016/j.talanta.2020.120961
23. [23]
  G. Choi, E. Kim, E. Park, J.H. Lee, Talanta 162 (2017) 38–45. doi: 10.1016/j.talanta.2016.09.061
24. [24]
  P. Preechakasedkit, K. Osada, Y. Katayama, et al., Analyst 143 (2018) 564–570. doi: 10.1039/C7AN01799E
1. [1]
  Hualei Xu , Manman Han , Haiqiang Liu , Liang Qin , Lulu Chen , Hao Hu , Ran Wu , Chenyu Yang , Hua Guo , Jinrong Li , Jinxiang Fu , Qichen Hao , Yijun Zhou , Jinchao Feng , Xiaodong Wang . 4-Nitrocatechol as a novel matrix for low-molecular-weight compounds in situ detection and imaging in biological tissues by MALDI-MSI. Chinese Chemical Letters, 2024, 35(6): 109095-. doi: 10.1016/j.cclet.2023.109095
2. [2]
  Zhixue Liu , Haiqi Chen , Lijuan Guo , Xinyao Sun , Zhi-Yuan Zhang , Junyi Chen , Ming Dong , Chunju Li . Luminescent terphen[3]arene sulfate-activated FRET assemblies for cell imaging. Chinese Chemical Letters, 2024, 35(9): 109666-. doi: 10.1016/j.cclet.2024.109666
3. [3]
  Jie XIE , Hongnan XU , Jianfeng LIAO , Ruoyu CHEN , Lin SUN , Zhong JIN . Nitrogen-doped 3D graphene-carbon nanotube network for efficient lithium storage. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1840-1849. doi: 10.11862/CJIC.20240216
4. [4]
  Xuejian Xing , Pan Zhu , E Pang , Shaojing Zhao , Yu Tang , Zheyu Hu , Quchang Ouyang , Minhuan Lan . D-A-D-structured boron-dipyrromethene with aggregation-induced enhanced phototherapeutic efficiency for near-infrared fluorescent and photoacoustic imaging-guided synergistic photodynamic and photothermal cancer therapy. Chinese Chemical Letters, 2024, 35(10): 109452-. doi: 10.1016/j.cclet.2023.109452
5. [5]
  Xiao-Hong Yi , Chong-Chen Wang . Metal-organic frameworks on 3D interconnected macroporous sponge foams for large-scale water decontamination: A mini review. Chinese Chemical Letters, 2024, 35(5): 109094-. doi: 10.1016/j.cclet.2023.109094
6. [6]
  Yi Zhu , Jingyan Zhang , Yuchao Zhang , Ying Chen , Guanghui An , Ren Liu . Designing unimolecular photoinitiator by installing NHPI esters along the TX backbone for acrylate photopolymerization and their applications in coatings and 3D printing. Chinese Chemical Letters, 2024, 35(7): 109573-. doi: 10.1016/j.cclet.2024.109573
7. [7]
  Jie Wu , Xiaoqing Yu , Guoxing Li , Su Chen . Engineering particles towards 3D supraballs-based passive cooling via grafting CDs onto colloidal photonic crystals. Chinese Chemical Letters, 2024, 35(4): 109234-. doi: 10.1016/j.cclet.2023.109234
8. [8]
  Yue Li , Minghao Fan , Conghui Wang , Yanxun Li , Xiang Yu , Jun Ding , Lei Yan , Lele Qiu , Yongcai Zhang , Longlu Wang . 3D layer-by-layer amorphous MoS_x assembled from [Mo₃S₁₃]^2- clusters for efficient removal of tetracycline: Synergy of adsorption and photo-assisted PMS activation. Chinese Chemical Letters, 2024, 35(9): 109764-. doi: 10.1016/j.cclet.2024.109764
9. [9]
  Hengying Xiang , Nanping Deng , Lu Gao , Wen Yu , Bowen Cheng , Weimin Kang . 3D core-shell nanofibers framework and functional ceramic nanoparticles synergistically reinforced composite polymer electrolytes for high-performance all-solid-state lithium metal battery. Chinese Chemical Letters, 2024, 35(8): 109182-. doi: 10.1016/j.cclet.2023.109182
10. [10]
  Jing-Jing Zhang , Lujun Lou , Rui Lv , Jiahui Chen , Yinlong Li , Guangwei Wu , Lingchao Cai , Steven H. Liang , Zhen Chen . Recent advances in photochemistry for positron emission tomography imaging. Chinese Chemical Letters, 2024, 35(8): 109342-. doi: 10.1016/j.cclet.2023.109342
11. [11]
  Shihong Wu , Ronghui Zhou , Hang Zhao , Peng Wu . Sonoafterglow luminescence for in vivo deep-tissue imaging. Chinese Chemical Letters, 2024, 35(10): 110026-. doi: 10.1016/j.cclet.2024.110026
12. [12]
  Kun Zhang , Ni Dan , Dan-Dan Ren , Ruo-Yu Zhang , Xiaoyan Lu , Ya-Pan Wu , Li-Lei Zhang , Hong-Ru Fu , Dong-Sheng Li . A small D-A molecule with highly heat-resisting room temperature phosphorescence for white emission and anti-counterfeiting. Chinese Journal of Structural Chemistry, 2024, 43(3): 100244-100244. doi: 10.1016/j.cjsc.2024.100244
13. [13]
  Xiaohong Wen , Mei Yang , Lie Li , Mingmin Huang , Wei Cui , Suping Li , Haiyan Chen , Chen Li , Qiuping Guo . Enzymatically controlled DNA tetrahedron nanoprobes for specific imaging of ATP in tumor. Chinese Chemical Letters, 2024, 35(8): 109291-. doi: 10.1016/j.cclet.2023.109291
14. [14]
  Gongcheng Ma , Qihang Ding , Yuding Zhang , Yue Wang , Jingjing Xiang , Mingle Li , Qi Zhao , Saipeng Huang , Ping Gong , Jong Seung Kim . Palladium-free chemoselective probe for in vivo fluorescence imaging of carbon monoxide. Chinese Chemical Letters, 2024, 35(9): 109293-. doi: 10.1016/j.cclet.2023.109293
15. [15]
  Botao QU , Qian WANG , Xiaogang NING , Yuxin ZHOU , Ruiping ZHANG . Deeply penetrating photoacoustic imaging in tumor tissues based on dual-targeted melanin nanoparticle. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 1025-1032. doi: 10.11862/CJIC.20230416
16. [16]
  Peng Jia , Yunna Guo , Dongliang Chen , Xuedong Zhang , Jingming Yao , Jianguo Lu , Liqiang Zhang . In-situ imaging electrocatalysis in a solid-state Li-O₂ battery with CuSe nanosheets as air cathode. Chinese Chemical Letters, 2024, 35(5): 108624-. doi: 10.1016/j.cclet.2023.108624
17. [17]
  Boran Cheng , Lei Cao , Chen Li , Fang-Yi Huo , Qian-Fang Meng , Ganglin Tong , Xuan Wu , Lin-Lin Bu , Lang Rao , Shubin Wang . Fluorine-doped carbon quantum dots with deep-red emission for hypochlorite determination and cancer cell imaging. Chinese Chemical Letters, 2024, 35(6): 108969-. doi: 10.1016/j.cclet.2023.108969
18. [18]
  Hui-Juan Wang , Wen-Wen Xing , Zhen-Hai Yu , Yong-Xue Li , Heng-Yi Zhang , Qilin Yu , Hongjie Zhu , Yao-Yao Wang , Yu Liu . Cucurbit[7]uril confined phenothiazine bridged bis(bromophenyl pyridine) activated NIR luminescence for lysosome imaging. Chinese Chemical Letters, 2024, 35(6): 109183-. doi: 10.1016/j.cclet.2023.109183
19. [19]
  Jingqi Xin , Shupeng Han , Meichen Zheng , Chenfeng Xu , Zhongxi Huang , Bin Wang , Changmin Yu , Feifei An , Yu Ren . A nitroreductase-responsive nanoprobe with homogeneous composition and high loading for preoperative non-invasive tumor imaging and intraoperative guidance. Chinese Chemical Letters, 2024, 35(7): 109165-. doi: 10.1016/j.cclet.2023.109165
20. [20]
  Yiling Li , Zekun Gao , Xiuxiu Yue , Minhuan Lan , Xiuli Zheng , Benhua Wang , Shuang Zhao , Xiangzhi Song . FRET-based two-photon benzo[a] phenothiazinium photosensitizer for fluorescence imaging-guided photodynamic therapy. Chinese Chemical Letters, 2024, 35(7): 109133-. doi: 10.1016/j.cclet.2023.109133

Get Citation

PDF

XML

Metrics

PDF Downloads(2)
Abstract views(204)
HTML views(10)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142