Citation: Peng Gao, Jiayun Chen, Peng Sun, Jianyou Wang, Huan Tang, Fei Xia, Liwei Gu, Huimin Zhang, Chen Wang, Yin Kwan Wong, Yinhua Zhu, Chengchao Xu, Jigang Wang. Chemical proteomic profiling with photoaffinity labeling strategy identifies antimalarial targets of artemisinin[J]. Chinese Chemical Letters, ;2023, 34(12): 108296. doi: 10.1016/j.cclet.2023.108296 shu

Chemical proteomic profiling with photoaffinity labeling strategy identifies antimalarial targets of artemisinin

Peng Gao ^{a, 1} ,
Jiayun Chen ^{a, 1} ,
Peng Sun ^{a, 1} ,
Jianyou Wang ^{b, 1} ,
Huan Tang ^{a, 1} ,
Fei Xia ^a ,
Liwei Gu ^a ,
Huimin Zhang ^c ,
Chen Wang ^a ,
Yin Kwan Wong ^a ,
Yinhua Zhu ^a ,
Chengchao Xu ^{a, *,} ,
Jigang Wang ^{a, c, d, *,}

a.
Artemisinin Research Center, and Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
b.
Pharmaceutical College, Henan University, Kaifeng 475004, China
c.
Shandong Academy of Chinese Medicine, Ji’nan 250014, China
d.
Department of Nephrology, Shenzhen Key Laboratory of Kidney Diseases, Shenzhen Clinical Research Centre for Geriatrics, Shenzhen People’s Hospital, The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen 518020, China

ccxu@icmm.ac.cn

jgwang@icmm.ac.cn

Received Date: 30 October 2022
Revised Date: 25 February 2023
Accepted Date: 2 March 2023
Available Online: 5 March 2023

Figures(4)

Present research on the antimalarial mechanisms of artemisinin (ART) is mainly focused on covalent drug binding targets alkylated by free radicals, while non-covalent binding targets have rarely been reported. Here, we developed a novel photoaffinity probe of ART to globally capture and identify the antimalarial target proteins of ART through chemical proteomics. The results demonstrated that ART can bind to parasite proteins by both covalent and non-covalent modification, and these may jointly contribute to the antimalarial effects. Our work enriches the research on the antimalarial targets of ART, and provides a new perspective for further exploring the antimalarial mechanism of ART.
- Artemisinin,
- Antimalarial,
- Chemical proteomic,
- Target identification,
- Photoaffinity probe
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