Citation: Nachuan Song, Shuai Li, Zhaoyue Lv, Xiaohui Ding, Feng Li, Dayong Yang. Engineering CRISPR/Cas-based nanosystems for therapeutics, diagnosis and bioimaging[J]. Chinese Chemical Letters, ;2023, 34(10): 108134. doi: 10.1016/j.cclet.2023.108134 shu

Engineering CRISPR/Cas-based nanosystems for therapeutics, diagnosis and bioimaging

Nachuan Song ^{a, 1} ,
Shuai Li ^{a, b, 1} ,
Zhaoyue Lv ^{a, 1} ,
Xiaohui Ding ^a ,
Feng Li ^{a, *,} ,
Dayong Yang ^{a, b, *,}

a.
Frontiers Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), Institute of Biomolecular and Biomedical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China
b.
Zhejiang Institute of Tianjin University, Ningbo 315201, China

fengli2018@tju.edu.cn

dayong.yang@tju.edu.cn

Received Date: 13 October 2022
Revised Date: 30 December 2022
Accepted Date: 3 January 2023
Available Online: 5 January 2023

Figures(5)

CRISPR/Cas system has been utilized to rationally manipulate intracellular genes, and it has been engineered as versatile and efficient gene editing tools with precise site-specificity and excellent targeting ability for therapeutics, diagnostics, and bioimaging. Here, the evolution and application of CRISPR/Cas systems were sketched chronologically. Landmark works were exemplified to illustrate the design principles of CRISPR/Cas systems. Furthermore, the delivery vectors of CRISPR/Cas system especially DNA nanomaterials-based vectors were categorized and illuminated. DNA nanomaterials are suitable for CRISPR/Cas system delivery via base pairing due to its sequence programmability and biocompatibility. Then the applications of CRISPR/Cas in diagnosis and genomic imaging were highlighted. At the end of the review, the challenges and opportunities of CRISPR/Cas systems were deeply discussed. We envision that the grant advances on CRISPR/Cas systems will promote the development of interdisciplinary fields in chemistry, biology and medicine.
- CRISPR/Cas,
- DNA nanotechnology,
- DNA nanomaterials,
- Cancer therapy,
- Diagnosis,
- Bioimaging
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