Citation: Haiyan Wang, Peng Liang, Lei Zhang, Liping Shi, Yitong Ge, Liyuan Zhang, Xiaoqiang Qiao. A soluble pH-responsive host-guest-based nanosystem for homogeneous exosomes capture with high-efficiency[J]. Chinese Chemical Letters, ;2023, 34(8): 108129. doi: 10.1016/j.cclet.2023.108129 shu

A soluble pH-responsive host-guest-based nanosystem for homogeneous exosomes capture with high-efficiency

Haiyan Wang ^{a, 1} ,
Peng Liang ^{a, 1} ,
Lei Zhang ^a ,
Liping Shi ^b ,
Yitong Ge ^b ,
Liyuan Zhang ^{b, *,} ,
Xiaoqiang Qiao ^{a, *,}

a.
College of Pharmaceutical Sciences, Key Laboratory of Public Health Safety of Hebei Province, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University, Baoding 071002, China
b.
College of Basic Medical Science, Key Laboratory for Proteomics of Liaoning Province, Dalian Medical University, Dalian 116044, China

zhiani@126.com

xiaoqiao@hbu.edu.cn

Received Date: 31 August 2022
Revised Date: 24 November 2022
Accepted Date: 1 January 2023
Available Online: 5 January 2023

Figures(5)

Exosomes offer ideal biomarkers for liquid biopsies. However, high-efficient capture of exosomes has been proven to be extreme challenging. Here, we report a soluble pH-responsive host-guest-based nanosystem (pH-HGN) for homogeneous isolation of exosomes around physiological pH. The pH-HGN consists of two specifically functionalized modules. First, a pH-responsive module, poly-dimethylaminoethyl methacrylate, provides homogeneous capture circumstances and sharp pH-triggered self-assembly separation in aqueous solution to improve capture efficiency and reduce nonspecific adsorption. Second, a host-guest module, poly-acrylamide azobenzene and β-cyclodextrin linked with exosomes-specific antibody, could act as the "cleavable bridge" to specific capture and subsequent rapid release of captured exosomes through host-guest interaction between β-cyclodextrin and AAAB moieties. The pH-HGN offered high capture efficiencies for exosomes from two different cell lines, which were 90.2% ± 0.28% and 87.0% ± 4.6% for H1299 and MCF-7 cell-derived exosomes, respectively. The purity of isolated exosomes was (1.49 ± 0.71) × 10¹¹ particles/μg, which was 4.1 times higher compared with the gold standard ultracentrifugation (UC) method. Furthermore, the isolated exosomes via the pH-HGN can preserve well integrity and biological activity. The developed pH-HGN was further successfully applied to differentiate lung cancer patients from healthy persons. These findings indicated that pH-HGN is a promising strategy in exosomes-based research and downstream applications.
- Exosomes,
- Homogeneous capture,
- Soluble nanosystem,
- pH-responsive polymer,
- Host-guest interaction
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