Citation: Xiongbo Song, Liling Gu, Qiming Yang, Jiarui Wu, Junrong Chen, Xiaobin Tian, Li Sun, Long Chen. Thermosensitive injectable hydrogel loaded with hypoxia-induced exosomes maintains chondrocyte phenotype through NDRG3-mediated hypoxic response[J]. Chinese Chemical Letters, ;2023, 34(8): 108079. doi: 10.1016/j.cclet.2022.108079 shu

Thermosensitive injectable hydrogel loaded with hypoxia-induced exosomes maintains chondrocyte phenotype through NDRG3-mediated hypoxic response

Xiongbo Song ^{a, b} ,
Liling Gu ^b ,
Qiming Yang ^b ,
Jiarui Wu ^b ,
Junrong Chen ^b ,
Xiaobin Tian ^{c, *,} ,
Li Sun ^{a, b, **,} ,
Long Chen ^{a, b, **,}

a.
Medical College, Guizhou University, Guiyang 550000, China
b.
Department of Orthopedics, Guizhou Provincial People’s Hospital, Guiyang 550000, China
c.
Department of Orthopaedics, The Affiliated Hospital of Guizhou Medical University, Guiyang 550000, China

^**

txb6@vip.163.com

lisun@gzu.edu.cn

longchen@gzu.edu.cn

Received Date: 31 August 2022
Revised Date: 13 December 2022
Accepted Date: 14 December 2022
Available Online: 16 December 2022

Figures(3)

Current clinical treatments cannot effectively delay the progression of osteoarthritis (OA). Consequently, joint replacement surgery is required for late-stage OA when patients cannot tolerate pain and joint dysfunction. Therefore, the prevention of OA progression in the early and middle stages is an urgent clinical problem. In a previous study, we demonstrated that NDRG3-mediated hypoxic response might be closely related to the development and progression of OA. In this study, an injectable thermosensitive hydrogel was established by cross-linking Pluronic F-127 and hyaluronic acid (HA) for the sustained release of hypoxia-induced exosomes (HExos) derived from adipose-derived mesenchymal stem cells. We demonstrated that for OA at the early and middle stages, the HExos-loaded HP hydrogel could maintain the chondrocyte phenotype by enhancing chondrocyte autophagy, reducing chondrocyte apoptosis, and promoting chondrocyte activity and proliferation through the NDRG3-mediated hypoxic response. This novel composite hydrogel, which could activate the NDRG3-mediated hypoxic response, may provide new ideas and a theoretical basis for the treatment of early- and mid-stage OA.
- Osteoarthritis,
- Injectable thermosensitive hydrogel,
- Exosomes,
- Hypoxic response,
- NDRG3
1. [1]
  K.D. Allen, L.M. Thoma, Y.M. Golightly, Osteoarthr. Cartil. 30 (2022) 184–195. doi: 10.1016/j.joca.2021.04.020
2. [2]
  L. Zheng, Z. Zhang, P. Sheng, A. Mobasheri, Ageing Res. Rev. 66 (2021) 101249. doi: 10.1016/j.arr.2020.101249
3. [3]
  A. Krishnamurthy, A.E. Lang, S. Pangarkar, et al., Mayo Clin. Proc. 96 (2021) 2435–2447. doi: 10.1016/j.mayocp.2021.03.017
4. [4]
  F.J. Zhang, W. Luo, G.H. Lei, Jt. Bone Spine 82 (2015) 144–147. doi: 10.1016/j.jbspin.2014.10.003
5. [5]
  K. Okada, D. Mori, Y. Makii, et al., Sci. Rep. 10 (2020) 5425. doi: 10.1038/s41598-020-62463-4
6. [6]
  W. Bouaziz, J. Sigaux, D. Modrowski, et al., Proc. Natl. Acad. Sci. U. S. A. 113 (2016) 5453–5458. doi: 10.1073/pnas.1514854113
7. [7]
  D.C. Lee, H.A. Sohn, Z.Y. Park, et al., Cell 161 (2015) 595–609. doi: 10.1016/j.cell.2015.03.011
8. [8]
  L. Chen, Y. Wang, S. Li, W. Zhou, L. Sun, Arthroplasty 3 (2021) 1. doi: 10.1186/s42836-020-00064-2
9. [9]
  M. Al-Najar, H. Khalil, J. Al-Ajlouni, et al., J. Orthop. Surg. Res. 12 (2017) 190. doi: 10.1186/s13018-017-0689-6
10. [10]
  J.M. Lamo-Espinosa, G. Mora, J.F. Blanco, et al., J. Transl. Med. 16 (2018) 213. doi: 10.1186/s12967-018-1591-7
11. [11]
  D. Garay-Mendoza, L. Villarreal-Martínez, A. Garza-Bedolla, et al., Int. J. Rheum. Dis. 21 (2018) 140–147. doi: 10.1111/1756-185x.13139
12. [12]
  Y. Song, H. Du, C. Dai, et al., Regen. Med. 13 (2018) 295–307. doi: 10.2217/rme-2017-0152
13. [13]
  L. He, T. He, J. Xing, et al., Stem Cell Res. Ther. 11 (2020) 276. doi: 10.1186/s13287-020-01781-w
14. [14]
  Y. Zhu, Y. Wang, B. Zhao, et al., Stem Cell Res. Ther. 8 (2017) 64. doi: 10.21832/9781845415907-007
15. [15]
  Y. Wang, D. Yu, Z. Liu, et al., Stem Cell Res. Ther. 8 (2017) 189. doi: 10.14257/ijhit.2017.10.1.17
16. [16]
  S. Zhang, K.Y.W. Teo, S.J. Chuah, et al., Biomaterials 200 (2019) 35–47. doi: 10.1016/j.biomaterials.2019.02.006
17. [17]
  M.H.M. Nascimento, M.K.K.D. Franco, F. Yokaichyia, et al., Int. J. Biol. Macromol. 111 (2018) 1245–1254. doi: 10.1016/j.ijbiomac.2018.01.064
18. [18]
  S. Bhattacharya, A. Ghosh, S. Maiti, et al., J. Control. Release 322 (2020) 357–374. doi: 10.1016/j.jconrel.2020.03.033
19. [19]
  N. Wei, X. Xu, C. Huang, L. Cao, A.A.P.S. Pharm. Sci. Tech. 21 (2020) 162. doi: 10.1208/s12249-020-01714-z
20. [20]
  Y.S. Jung, W. Park, H. Park, D.K. Lee, K. Na, Carbohydr. Polym. 156 (2017) 403–408. doi: 10.1016/j.carbpol.2016.08.068
21. [21]
  Y.S. Kim, F. Guilak, Int. J. Mol. Sci. 23 (2022) 8662. doi: 10.3390/ijms23158662
22. [22]
  M. Torres-Torrillas, M. Rubio, E. Damia, et al., Int. J. Mol Sci. 20 (2019) 3105. doi: 10.3390/ijms20123105
23. [23]
  A. Hassanshahi, M. Hassanshahi, S. Khabbazi, et al., J. Cell. Physiol. 234 (2019) 7903–7914. doi: 10.1002/jcp.27922
24. [24]
  D. Murata, R. Fujimoto, K. Nakayama, Int. J. Mol. Sci. 21 (2020) 3589. doi: 10.3390/ijms21103589
25. [25]
  L. Mazini, L. Rochette, M. Amine, G. Malka, Int. J. Mol. Sci. 20 (2019) 2523. doi: 10.3390/ijms20102523
26. [26]
  X. Sang, X. Zhao, L. Yan, et al., Tissue Eng. Regen. Med. 19 (2022) 629–642. doi: 10.1007/s13770-022-00437-5
27. [27]
  H. Katagiri, L.F. Mendes, F.P. Luyten, Osteoarthr. Cartil. 25 (2017) 1531–1540. doi: 10.1016/j.joca.2017.05.006
28. [28]
  X. Ouyang, Y. Ding, L. Yu, et al., Clin. Immunol. 233 (2021) 108889. doi: 10.1016/j.clim.2021.108889
29. [29]
  R.R. Yammani, R.F. Loeser, Arthr. Rheumatol. 66 (2014) 1266–1271. doi: 10.1002/art.38391
30. [30]
  G. Han, H. Shao, X. Zhu, et al., Carbohydr. Polym. 89 (2012) 870–875. doi: 10.1016/j.carbpol.2012.04.023
31. [31]
  M.K. Lotz, B. Caramés, Nat. Rev. Rheumatol. 7 (2011) 579–587. doi: 10.1038/nrrheum.2011.109
32. [32]
  M.T. Valenti, L. Dalle Carbonare, D. Zipeto, M. Mottes, Int. J. Mol. Sci. 22 (2021) 2700. doi: 10.3390/ijms22052700
33. [33]
  B. Caramés, N. Taniguchi, S. Otsuki, F.J. Blanco, M. Lotz, Arthr. Rheum. 62 (2010) 791–801. doi: 10.1002/art.27305
34. [34]
  N. Deleyto-Seldas, A. Efeyan, Front. Cell. Dev. Biol. 9 (2021) 655731. doi: 10.3389/fcell.2021.655731
35. [35]
  J.F. Xue, Z.M. Shi, J. Zou, X.L. Li, Biomed. Pharmacother. 89 (2017) 1252–1261. doi: 10.1016/j.biopha.2017.01.130
36. [36]
  K. Sun, J. Luo, J. Guo, et al., Osteoarthr. Cartil. 28 (2020) 400–409. doi: 10.1016/j.joca.2020.02.027
1. [1]
  Fengjie Liu , Fansu Meng , Zhenjiang Yang , Huan Wang , Yuehong Ren , Yu Cai , Xingwang Zhang . Exosome-biomimetic nanocarriers for oral drug delivery. Chinese Chemical Letters, 2024, 35(9): 109335-. doi: 10.1016/j.cclet.2023.109335
2. [2]
  Jingwen Zhao , Jianpu Tang , Zhen Cui , Limin Liu , Dayong Yang , Chi Yao . A DNA micro-complex containing polyaptamer for exosome separation and wound healing. Chinese Chemical Letters, 2024, 35(9): 109303-. doi: 10.1016/j.cclet.2023.109303
3. [3]
  Ningyue Xu , Jun Wang , Lei Liu , Changyang Gong . Injectable hydrogel-based drug delivery systems for enhancing the efficacy of radiation therapy: A review of recent advances. Chinese Chemical Letters, 2024, 35(8): 109225-. doi: 10.1016/j.cclet.2023.109225
4. [4]
  Cuiwu MO , Gangmin ZHANG , Chao WU , Zhipeng HUANG , Chi ZHANG . A(NH₂SO₃) (A=Li, Na): Two ultraviolet transparent sulfamates exhibiting second harmonic generation response. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1387-1396. doi: 10.11862/CJIC.20240045
5. [5]
  Ruikui YAN , Xiaoli CHEN , Miao CAI , Jing REN , Huali CUI , Hua YANG , Jijiang WANG . Design, synthesis, and fluorescence sensing performance of highly sensitive and multi-response lanthanide metal-organic frameworks. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 834-848. doi: 10.11862/CJIC.20230301
6. [6]
  Yuanjiao Liu , Xiaoyang Zhao , Songyao Zhang , Yi Wang , Yutuo Zheng , Xinrui Miao , Wenli Deng . Site-selection and recognition of aromatic carboxylic acid in response to coronene and pyridine derivative. Chinese Chemical Letters, 2024, 35(8): 109404-. doi: 10.1016/j.cclet.2023.109404
7. [7]
  Xiao-Tong Sun , Hao-Fei Ni , Yi Zhang , Da-Wei Fu . Hybrid perovskite shows temperature-dependent photoluminescence and dielectric response triggered by halogen substitution. Chinese Journal of Structural Chemistry, 2024, 43(6): 100212-100212. doi: 10.1016/j.cjsc.2024.100212
8. [8]
  Ying Zhao , Yin-Hang Chai , Tian Chen , Jie Zheng , Ting-Ting Li , Francisco Aznarez , Li-Long Dang , Lu-Fang Ma . Size-controlled synthesis and near-infrared photothermal response of Cp* Rh-based metalla[2]catenanes and rectangular metallamacrocycles. Chinese Chemical Letters, 2024, 35(6): 109298-. doi: 10.1016/j.cclet.2023.109298
9. [9]
  Xiaoliu Liang , Chunliu Huang , Hui Liu , Hu Chen , Jiabao Shou , Hongwei Cheng , Gang Liu . Natural hydrogel dressings in wound care: Design, advances, and perspectives. Chinese Chemical Letters, 2024, 35(10): 109442-. doi: 10.1016/j.cclet.2023.109442
10. [10]
  Jianye Kang , Xinyu Yang , Xuhao Yang , Jiahui Sun , Yuhang Liu , Shutao Wang , Wenlong Song . Carbon dots-enhanced pH-responsive lubricating hydrogel based on reversible dynamic covalent bondings. Chinese Chemical Letters, 2024, 35(5): 109297-. doi: 10.1016/j.cclet.2023.109297
11. [11]
  Xin Li , Xuan Ding , Junkun Zhou , Hui Shi , Zhenxi Dai , Jiayi Liu , Yongcun Ma , Penghui Shao , Liming Yang , Xubiao Luo . Utilizing synergistic effects of bifunctional polymer hydrogel PAM-PAMPS for selective capture of Pb(Ⅱ) from wastewater. Chinese Chemical Letters, 2024, 35(7): 109158-. doi: 10.1016/j.cclet.2023.109158
12. [12]
  Xinyue Lan , Junguang Liang , Churan Wen , Xiaolong Quan , Huimin Lin , Qinqin Xu , Peixian Chen , Guangyu Yao , Dan Zhou , Meng Yu . Photo-manipulated polyunsaturated fatty acid-doped liposomal hydrogel for flexible photoimmunotherapy. Chinese Chemical Letters, 2024, 35(4): 108616-. doi: 10.1016/j.cclet.2023.108616
13. [13]
  Yu Yan , Jiawei Song , Dongdong Liu , Zihan Liu , Jialing Cheng , Zhiyang Chen , Yanfang Yang , Weizhe Jiang , Hongliang Wang , Jun Ye , Yuling Liu . Simple and versatile in situ thermo-sensitive hydrogel for rectal administration of SZ-A to alleviate inflammation and repair mucosal barrier in ulcerative colitis. Chinese Chemical Letters, 2024, 35(6): 109736-. doi: 10.1016/j.cclet.2024.109736
14. [14]
  Ningning Gao , Yue Zhang , Zhenhao Yang , Lijing Xu , Kongyin Zhao , Qingping Xin , Junkui Gao , Junjun Shi , Jin Zhong , Huiguo Wang . Ba²⁺/Ca²⁺ co-crosslinked alginate hydrogel filtration membrane with high strength, high flux and stability for dye/salt separation. Chinese Chemical Letters, 2024, 35(5): 108820-. doi: 10.1016/j.cclet.2023.108820
15. [15]
  Gregorio F. Ortiz . Some facets of the Mg/Na₃VCr_0.5Fe_0.5(PO₄)₃ battery. Chinese Chemical Letters, 2024, 35(10): 109391-. doi: 10.1016/j.cclet.2023.109391
16. [16]
  Juan Guo , Mingyuan Fang , Qingsong Liu , Xiao Ren , Yongqiang Qiao , Mingju Chao , Erjun Liang , Qilong Gao . Zero thermal expansion in Cs₂W₃O₁₀. Chinese Chemical Letters, 2024, 35(7): 108957-. doi: 10.1016/j.cclet.2023.108957
17. [17]
  Jing JIN , Zhuming GUO , Zhiyin XIAO , Xiujuan JIANG , Yi HE , Xiaoming LIU . Tuning the stability and cytotoxicity of fac-[Fe(CO)₃I₃]^- anion by its counter ions: From aminiums to inorganic cations. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 991-1004. doi: 10.11862/CJIC.20230458
18. [18]
  Jisheng Liu , Junli Chen , Xifeng Zhang , Yin Wu , Xin Qi , Jie Wang , Xiang Gao . Red blood cell membrane-coated FLT3 inhibitor nanoparticles to enhance FLT3-ITD acute myeloid leukemia treatment. Chinese Chemical Letters, 2024, 35(9): 109779-. doi: 10.1016/j.cclet.2024.109779
19. [19]
  Kun Zou , Yihang Xiao , Jinyu Yang , Mingxuan Wu . Facile semisynthesis of histone H3 enables nucleosome probes for investigation of histone H3K79 modifications. Chinese Chemical Letters, 2024, 35(10): 109497-. doi: 10.1016/j.cclet.2024.109497
20. [20]
  Min WANG , Dehua XIN , Yaning SHI , Wenyao ZHU , Yuanqun ZHANG , Wei ZHANG . Construction and full-spectrum catalytic performance of multilevel Ag/Bi/nitrogen vacancy g-C₃N₄/Ti₃C₂T_x Schottky junction. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1123-1134. doi: 10.11862/CJIC.20230477

Get Citation

PDF

XML

Metrics

PDF Downloads(3)
Abstract views(130)
HTML views(1)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142