Citation: Delu Che, Chenfei Wang, Zhili Li, Kaixuan Wang, Shuaiwei Sun, Xinyue Zhang, Yi Li, Zhengju Chen, Lei Guo, Yajing Hou, Dezhong Zhou, Songmei Geng. Efficient gene transfection of suspension cells by highly branched poly(β-amino ester)[J]. Chinese Chemical Letters, ;2023, 34(7): 108066. doi: 10.1016/j.cclet.2022.108066 shu

Efficient gene transfection of suspension cells by highly branched poly(β-amino ester)

Delu Che ^{a, 1} ,
Chenfei Wang ^{b, 1,} ,
Zhili Li ^{b, 1} ,
Kaixuan Wang ^{b, 1} ,
Shuaiwei Sun ^b ,
Xinyue Zhang ^a ,
Yi Li ^{c, *,} ,
Zhengju Chen ^d ,
Lei Guo ^d ,
Yajing Hou ^e ,
Dezhong Zhou ^{b, *,} ,
Songmei Geng ^{a, *,}

a.
Department of Dermatology, the Second Hospital Affiliated to Xi'an Jiaotong University, Xi'an 710061, China
b.
School of Chemical Engineering and Technology, Xi'an Jiaotong University, Xi'an 710049, China
c.
Infectious Disease Department, the Second Xiangya Hospital of Central South University, Changsha 410011, China
d.
Pooling Medical Research Institutes, Hangzhou 310053, China
e.
Department of Pharmacy, Shaanxi Provincial People's Hospital, Xi'an 710068, China

chenfeiwang0120@stu.xjtu.edu.cn

liyi731128@csu.edu.cn

dezhong.zhou@xjtu.edu.cn

gsm312@yahoo.com

Received Date: 6 June 2022
Revised Date: 11 December 2022
Accepted Date: 12 December 2022
Available Online: 15 December 2022

Figures(7)

Suspension cells play a crucial role in many biological processes. However, compared to adherent cells, it is particularly challenging to introduce exogenous genes into suspension cells to regulate their biological functions with non-viral gene vectors, mainly due to the low cellular uptake and endosomal escape of polyplexes. Herein, to improve the interactions of polyplexes with cellular membranes, we design and synthesize highly branched poly(β-amino ester) (HPAE) via an "A2 + B4 + C2" Michael addition strategy. Results show that branching significantly increases DNA condensation of HPAE, cellular uptake and endosomal escape of HPAE/DNA polyplexes. In mast cells (MCs), HPAE exhibits up to 80-fold higher gene transfection efficiency compared to the corresponding linear poly(β-amino ester) (LPAE) and the leading commercial gene transfection reagents PEI25k, jetPEI, and Lipofectamine 3000, without causing obvious cytotoxicity. Our study establishes a reliable non-viral platform for efficient gene transfection of suspension cells.
- Non-viral vector,
- Highly branched poly(β-amino ester),
- Gene transfection,
- High transfection efficiency,
- Suspension cells,
- Mast cells
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