Citation: Jianfeng Ma, Lingbo Gong, Yingying Cen, Lin Feng, Yan Su, Xingfen Liu, Jie Chao, Ying Wan, Shao Su, Lianhui Wang. Electrochemical analysis of microRNAs with hybridization chain reaction-based triple signal amplification[J]. Chinese Chemical Letters, ;2023, 34(7): 108012. doi: 10.1016/j.cclet.2022.108012 shu

Electrochemical analysis of microRNAs with hybridization chain reaction-based triple signal amplification

Jianfeng Ma ^a ,
Lingbo Gong ^a ,
Yingying Cen ^a ,
Lin Feng ^a ,
Yan Su ^b ,
Xingfen Liu ^a ,
Jie Chao ^a ,
Ying Wan ^{b, *,} ,
Shao Su ^{a, *,} ,
Lianhui Wang ^{a, *,}

a.
State Key Laboratory of Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, Nanjing 210023, China
b.
School of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China

wanying@njust.edu.cn

iamssu@njupt.edu.cn

iamlhwang@njupt.edu.cn

Received Date: 2 July 2022
Revised Date: 5 November 2022
Accepted Date: 20 November 2022
Available Online: 26 November 2022

Figures(5)

Selective and sensitive detection of trace microRNA is important for early diagnosis of diseases due to its expression level related to diseases. Herein, a triple signal amplification strategy is developed for trace microRNA-21 (miRNA-21) detection by combining with target-triggered cyclic strand displacement reaction (TCSDR), hybridization chain reaction (HCR) and enzyme catalytic amplification. Four DNA hairpins (H1, H2, H3, H4) are employed to form an ultralong double-strand DNA (dsDNA) structure, which is initiated by target miRNA-21. As H3 and H4 are labeled with horseradish peroxidase (HRP), numerous HRPs are loaded on the long dsDNA, producing significantly enhanced electrocatalytic signals in the hydrogen peroxide (H₂O₂) and 3,3′,5,5′-tetramethylbenzidine (TMB) reaction strategy. Compared with single signal amplification, the triple signal amplification strategy shows higher electrochemical response, wider dynamic range and lower detection limit for miRNA-21 detection with excellent selectivity, reproducibility and stability. Taking advantage of the triple signal amplification strategy, the proposed electrochemical biosensor can detect miRNA-21 in 10 HeLa cell lysates, suggesting that it is a promising method for fruitful assay in clinical diagnosis.
- Electrochemical,
- Biosensor,
- microRNAs,
- Hybridization chain reaction,
- Target-triggered cyclic strand displacement reaction,
- Triple signal amplification
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沈阳化工大学材料科学与工程学院沈阳 110142