Citation: Ting Hou, Ningning Xu, Xin Song, Limin Yang, Feng Li. Label-free homogeneous photoelectrochemical aptasensing of VEGF₁₆₅ based on DNA-regulated peroxidase-mimetic activity of metal-organic-frameworks[J]. Chinese Chemical Letters, ;2023, 34(6): 107907. doi: 10.1016/j.cclet.2022.107907 shu

Label-free homogeneous photoelectrochemical aptasensing of VEGF₁₆₅ based on DNA-regulated peroxidase-mimetic activity of metal-organic-frameworks

College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, China

yanglimin@qau.edu.cn

lifeng@qau.edu.cn

Received Date: 1 August 2022
Revised Date: 29 September 2022
Accepted Date: 16 October 2022
Available Online: 18 October 2022

Figures(5)

The application of metal-organic frameworks (MOFs) nanozymes in biosensing has been extensively investigated, however, till now there is still no report on photoelectrochemical (PEC) sensing based on enzyme memetic properties of MOFs. To further expand the utilization of MOFs nanozymes in biosensing, we developed a label-free homogenous PEC aptasensor for the detection of VEGF₁₆₅, an important cancer biomarker, based on the DNA-regulated peroxidase-mimetic activity of Fe-MIL-88, a type of MOFs. In this strategy, the peroxidase-mimetic property of MOFs is integrated with the label-free homogeneous PEC sensing approach, and highly sensitive detection of VEGF₁₆₅ is obtained with a detection limit down to 33 fg/mL, superior or comparable to the previously reported values. Moreover, this approach displays outstanding specificity, and has been successfully used to detect VEGF₁₆₅ added in diluted serum samples. As far as we know, it is the first example to employ the peroxidase-like activity of MOFs in PEC biosensing, which may find potential application in bioanalysis and early disease diagnosis.
- Homogenous photoelectrochemistry,
- Aptasensing,
- Label free,
- VEGF₁₆₅,
- Fe-MIL-88,
- Peroxidase-like activity
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沈阳化工大学材料科学与工程学院沈阳 110142

Label-free homogeneous photoelectrochemical aptasensing of VEGF165 based on DNA-regulated peroxidase-mimetic activity of metal-organic-frameworks

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通讯作者: 陈斌, bchen63@163.com

Label-free homogeneous photoelectrochemical aptasensing of VEGF₁₆₅ based on DNA-regulated peroxidase-mimetic activity of metal-organic-frameworks