Citation: Na Wang, Yongjian Jiang, Kunhan Nie, Di Li, Hui Liu, Jian Wang, Chengzhi Huang, Chunmei Li. Toehold-mediated strand displacement reaction-propelled cascade DNAzyme amplifier for microRNA let-7a detection[J]. Chinese Chemical Letters, ;2023, 34(6): 107906. doi: 10.1016/j.cclet.2022.107906 shu

Toehold-mediated strand displacement reaction-propelled cascade DNAzyme amplifier for microRNA let-7a detection

Na Wang ^{a, 1} ,
Yongjian Jiang ^{a, 1} ,
Kunhan Nie ^a ,
Di Li ^b ,
Hui Liu ^a ,
Jian Wang ^a ,
Chengzhi Huang ^a ,
Chunmei Li ^{a, *,}

a.
Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China
b.
The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China

licm1024@swu.edu.cn

Received Date: 13 June 2022
Revised Date: 4 October 2022
Accepted Date: 14 October 2022
Available Online: 17 October 2022

Figures(6)

DNAzyme amplifiers have been extensively explored as a useful sensing platform, but single DNAzyme amplifier is limited in biosensing applications by its low sensitivity. Herein, a cascade DNAzyme amplifier was designed by exploiting concurrent amplification cycle principles of toehold-mediated strand displacement reaction (TSDR) and Zn²⁺-assisted DNAzyme cycle with lower cost and simpler procedures. Compared with single DNAzyme amplifier, the proposed TSDR-propelled cascade DNAzyme amplifier exhibited higher sensitivity by releasing more DNAzyme through TSDR to cleave substrate strand during the DNAzyme cycle. Base on this, let-7a could be sensitively detected in the range of 5–50 nmol/L with a detection limit of 64 pmol/L. Furthermore, the dual signal amplification strategy of the cascade DNAzyme amplifier exhibited excellent selectivity to distinguish single-base mismatched DNA strands, which has been successfully applied to the determination of let-7a in blood serum, showing high promise in early cancer diagnosis.
- DNAzyme amplifier,
- Toehold-mediated strand displacement reaction,
- Signal amplification,
- let-7a,
- Cancer marker
1. [1]
  J. Li, J.L. Wang, S.Y. Liu, et al., Angew. Chem. Int. Ed. 59 (2020) 20104–20111. doi: 10.1002/anie.202008245
2. [2]
  R. Garcia-Martin, G. Wang, B.B. Brandao, et al., Nature 601 (2022) 446–451. doi: 10.1038/s41586-021-04234-3
3. [3]
  M. Hou, D.G. He, H.Z. Wang, et al., Chin. Chem. Lett. 33 (2022) 3183–3187. doi: 10.1016/j.cclet.2021.11.092
4. [4]
  J. Hu, M.H. Liu, C.Y. Zhang, Chem. Sci. 9 (2018) 4258–4267. doi: 10.1039/C8SC00832A
5. [5]
  Q. Zhang, Y. Han, C.C. Li, et al., Chem. Commun. 58 (2022) 5538–5541. doi: 10.1039/D2CC01341J
6. [6]
  M. Cevec, C. Thibaudeau, J. Plavec, Nucleic Acids Res. 38 (2010) 7814–7821. doi: 10.1093/nar/gkq640
7. [7]
  W.M. Liu, R.R. Cheng, Z.R. Niu, et al., Sci. Adv. 6 (2020) e7070. doi: 10.1126/sciadv.aaz7070
8. [8]
  Y.S. Kim, H.J. Nam, C.Y. Han, et al., Hepatology 73 (2021) 1307–1326. doi: 10.1002/hep.31423
9. [9]
  Y. Yu, L. Liao, B.Y. Shao, et al., Mol. Ther. 25 (2017) 480–493. doi: 10.1016/j.ymthe.2016.11.015
10. [10]
  X.J. Luo, J.T. Zhu, W.Y. Jia, et al., ACS Appl. Mater. Interfaces 13 (2021) 18301–18313. doi: 10.1021/acsami.1c01834
11. [11]
  M. Daneshpour, B. Karimi, K. Omidfar, Biosens. Bioelectron. 109 (2018) 197–205. doi: 10.1016/j.bios.2018.03.022
12. [12]
  W.Y. Nie, Q. Wang, L.Y. Zou, et al., Anal. Chem. 90 (2018) 12584–12591. doi: 10.1021/acs.analchem.8b02686
13. [13]
  W.W. Chen, X.B. Zhang, J.J. Li, et al., Anal. Chem. 92 (2020) 2714–2721. doi: 10.1021/acs.analchem.9b04909
14. [14]
  X.T. Shen, Y. Zhang, J.H. Sun, et al., Chem. Sci. 10 (2019) 6113–6119. doi: 10.1039/C9SC01894H
15. [15]
  C.C. Li, J.P. Hu, X.R. Zou, X.L. Luo, C.Y. Zhang, Anal. Chem. 94 (2022) 1882–1889. doi: 10.1021/acs.analchem.1c05066
16. [16]
  P.J.J. Huang, J.W. Liu, J. Anal. Test. 6 (2021) 20–27. doi: 10.1117/12.2603258
17. [17]
  Q. Zhang, C.C. Li, F. Ma, X.L. Luo, C.Y. Zhang, Biosens. Bioelectron. 213 (2022) 114447. doi: 10.1016/j.bios.2022.114447
18. [18]
  D. Giust, M.I. Lucio, A.H. El-Sagheer, et al., ACS Nano 12 (2018) 6273–6279. doi: 10.1021/acsnano.8b03261
19. [19]
  C. Xing, S. Chen, Q.T. Lin, et al., Nanoscale 14 (2022) 1327–1332. doi: 10.1039/D1NR06399E
20. [20]
  Y.J. Jiang, N. Wang, F. Cheng, et al., Anal. Chem. 92 (2020) 11565–11572. doi: 10.1021/acs.analchem.0c00307
21. [21]
  M.K. Fan, C.Z. Huang, J. Anal. Test. 5 (2021) 195–196. doi: 10.1007/s41664-021-00196-7
22. [22]
  T.L. Kang, J.T. Zhu, X.J. Luo, et al., Anal. Chem. 93 (2021) 2519–2526. doi: 10.1021/acs.analchem.0c04561
23. [23]
  Y.T. Xu, Y.F. Ruan, H.Y. Wang, et al., Small 17 (2021) e2100503. doi: 10.1002/smll.202100503
24. [24]
  Z.L. Yang, K.Y. Loh, Y.T. Chu, et al., J. Am. Chem. Soc. 140 (2018) 17656–17665. doi: 10.1021/jacs.8b09867
25. [25]
  K. Quan, J. Li, J.L. Wang, et al., Chem. Sci. 10 (2019) 1442–1449. doi: 10.1039/C8SC04887H
26. [26]
  Y.J. Wang, K. Nguyen, R.C. Spitale, J.C. Chaput, Nat. Chem. 13 (2021) 319–326. doi: 10.1038/s41557-021-00645-x
27. [27]
  H.D. Qi, Y.W. Xu, P. Hu, C. Yao, D.Y. Yang, Chin. Chem. Lett. 33 (2022) 1131–1140. doi: 10.1016/j.cclet.2021.09.026
28. [28]
  W. Yun, H.X. Zhong, S. Zheng, R.Q. Wang, L.Z. Yang, Sens. Actuator B: Chem. 277 (2018) 456–461. doi: 10.1016/j.snb.2018.09.050
29. [29]
  Q.T. Lin, J.Y. Wu, L.L. Jiang, et al., Analyst 147 (2022) 262–267. doi: 10.1039/D1AN02036F
30. [30]
  J. Huang, W.J. Ma, H.H. Sun, et al., ACS Appl. Bio Mater. 3 (2020) 2779–2795. doi: 10.1021/acsabm.9b01197
31. [31]
  Y.J. Zhou, S.S. Yu, J.H. Shang, et al., Anal. Chem. 92 (2020) 15069–15078. doi: 10.1021/acs.analchem.0c03073
32. [32]
  J.F. Pan, Y. He, Z. Liu, J.H. Chen, Chem. Commun. 57 (2021) 1125–1128. doi: 10.1039/D0CC06799G
33. [33]
  X.J. Cui, R.G. Li, X.F. Liu, et al., Anal. Chim. Acta 997 (2018) 1–8. doi: 10.1016/j.aca.2017.10.009
34. [34]
  S.T. Li, Y. Zhang, J.J. Tian, W.T. Xu, Food Chem. 324 (2020) e126859. doi: 10.1016/j.foodchem.2020.126859
35. [35]
  S.F. Liu, C.B. Cheng, T. Liu, et al., Biosens. Bioelectron. 63 (2015) 99–104. doi: 10.1016/j.bios.2014.07.023
36. [36]
  J.A. Hambalek, J.E. Kong, C. Brown, et al., ACS Sens. 6 (2021) 3242–3252. doi: 10.1021/acssensors.1c00902
37. [37]
  M.Q. Huang, E.H. Xiong, Y. Wang, et al., Nat. Commun. 13 (2022) e968. doi: 10.1038/s41467-022-28627-8
38. [38]
  Y.J. Jiang, X.J. Yang, J. Wang, et al., Analyst 146 (2021) 7187–7193. doi: 10.1039/D1AN01642C
39. [39]
  X.Y. Wang, Y.F. Li, S.Z. Lv, S. Bi, J. Anal. Test. 6 (2022) 12–19. doi: 10.1007/s41664-021-00205-9
40. [40]
  F. Ma, Q. Zhang, C.Y. Zhang, Nano Lett. 19 (2019) 6370–6376. doi: 10.1021/acs.nanolett.9b02544
1. [1]
  Min Huang , Ru Cheng , Shuai Wen , Liangtong Li , Jie Gao , Xiaohui Zhao , Chunmei Li , Hongyan Zou , Jian Wang . Ultrasensitive detection of microRNA-21 in human serum based on the confinement effect enhanced chemical etching of gold nanorods. Chinese Chemical Letters, 2024, 35(9): 109379-. doi: 10.1016/j.cclet.2023.109379
2. [2]
  Kuangdi Luo , Yang Qin , Xuehao Zhang , Hanxu Ji , Heao Zhang , Jiangtian Li , Xianjin Xiao , Xinyu Wang . Regulable toehold lock for the effective control of strand displacement reaction sequence and circuit leakage. Chinese Chemical Letters, 2024, 35(7): 109104-. doi: 10.1016/j.cclet.2023.109104
3. [3]
  Yanqi Wu , Yuhong Guan , Peilin Huang , Hui Chen , Liping Bai , Zhihong Jiang . Preparation of norovirus GII loop mediated isothermal amplification freeze-drying microsphere reagents and its application in an on-site integrated rapid detection platform. Chinese Chemical Letters, 2024, 35(9): 109308-. doi: 10.1016/j.cclet.2023.109308
4. [4]
  Yiran Tao , Chunlei Dai , Zhaoxiang Xie , Xinru You , Kaiwen Li , Jun Wu , Hai Huang . Redox responsive polymeric nanoparticles enhance the efficacy of cyclin dependent kinase 7 inhibitor for enhanced treatment of prostate cancer. Chinese Chemical Letters, 2024, 35(8): 109170-. doi: 10.1016/j.cclet.2023.109170
5. [5]
  Yang Qin , Jiangtian Li , Xuehao Zhang , Kaixuan Wan , Heao Zhang , Feiyang Huang , Limei Wang , Hongxun Wang , Longjie Li , Xianjin Xiao . Toeless and reversible DNA strand displacement based on Hoogsteen-bond triplex. Chinese Chemical Letters, 2024, 35(5): 108826-. doi: 10.1016/j.cclet.2023.108826
6. [6]
  Minghao Hu , Tianci Xie , Yuqiang Hu , Longjie Li , Ting Wang , Tongbo Wu . Allosteric DNAzyme-based encoder for molecular information transfer. Chinese Chemical Letters, 2024, 35(7): 109232-. doi: 10.1016/j.cclet.2023.109232
7. [7]
  Neng Shi , Haonan Jia , Jixiang Zhang , Pengyu Lu , Chenglong Cai , Yixin Zhang , Liqiang Zhang , Nongyue He , Weiran Zhu , Yan Cai , Zhangqi Feng , Ting Wang . Accurate expression of neck motion signal by piezoelectric sensor data analysis. Chinese Chemical Letters, 2024, 35(9): 109302-. doi: 10.1016/j.cclet.2023.109302
8. [8]
  Junjie Wang , Yan Wang , Zhengdong Li , Changqiang Xie , Musammir Khan , Xingzhou Peng , Fabiao Yu . Triphenylamine-AIEgens photoactive materials for cancer theranostics. Chinese Chemical Letters, 2024, 35(6): 108934-. doi: 10.1016/j.cclet.2023.108934
9. [9]
  Fei Yin , Erli Yang , Xue Ge , Qian Sun , Fan Mo , Guoqiu Wu , Yanfei Shen . Coupling WO₃₋_x dots-encapsulated metal-organic frameworks and template-free branched polymerization for dual signal-amplified electrochemiluminescence biosensing. Chinese Chemical Letters, 2024, 35(4): 108753-. doi: 10.1016/j.cclet.2023.108753
10. [10]
  Yiqian Jiang , Zihan Yang , Xiuru Bi , Nan Yao , Peiqing Zhao , Xu Meng . Mediated electron transfer process in α-MnO₂ catalyzed Fenton-like reaction for oxytetracycline degradation. Chinese Chemical Letters, 2024, 35(8): 109331-. doi: 10.1016/j.cclet.2023.109331
11. [11]
  Huijie An , Chen Yang , Zhihui Jiang , Junjie Yuan , Zhongming Qiu , Longhao Chen , Xin Chen , Mutu Huang , Linlang Huang , Hongju Lin , Biao Cheng , Hongjiang Liu , Zhiqiang Yu . Luminescence-activated Pt(Ⅳ) prodrug for in situ triggerable cancer therapy. Chinese Chemical Letters, 2024, 35(7): 109134-. doi: 10.1016/j.cclet.2023.109134
12. [12]
  Mengjuan Sun , Muye Zhou , Yifang Xiao , Hailei Tang , Jinhua Chen , Ruitao Zhang , Chunjiayu Li , Qi Ya , Qian Chen , Jiasheng Tu , Qiyue Wang , Chunmeng Sun . Reversibly size-switchable polyion complex micelles for antiangiogenic cancer therapy. Chinese Chemical Letters, 2024, 35(7): 109110-. doi: 10.1016/j.cclet.2023.109110
13. [13]
  Haijing Cui , Weihao Zhu , Chuning Yue , Ming Yang , Wenzhi Ren , Aiguo Wu . Recent progress of ultrasound-responsive titanium dioxide sonosensitizers in cancer treatment. Chinese Chemical Letters, 2024, 35(10): 109727-. doi: 10.1016/j.cclet.2024.109727
14. [14]
  Hui-Juan Wang , Wen-Wen Xing , Zhen-Hai Yu , Yong-Xue Li , Heng-Yi Zhang , Qilin Yu , Hongjie Zhu , Yao-Yao Wang , Yu Liu . Cucurbit[7]uril confined phenothiazine bridged bis(bromophenyl pyridine) activated NIR luminescence for lysosome imaging. Chinese Chemical Letters, 2024, 35(6): 109183-. doi: 10.1016/j.cclet.2023.109183
15. [15]
  Huijiao Fu , Peiqin Liang , Qianwen Chen , Yan Wang , Guang Li , Xuzi Cai , Shengtao Wang , Kun Chen , Shengying Shi , Zhiqiang Yu , Xuefeng Wang . COX-2 blocking therapy in cisplatin chemosensitization of ovarian cancer: An allicin-based nanomedicine approach. Chinese Chemical Letters, 2024, 35(8): 109241-. doi: 10.1016/j.cclet.2023.109241
16. [16]
  Yang Liu , Yan Liu , Kaiyin Yang , Zhiruo Zhang , Wenbo Zhang , Bingyou Yang , Hua Li , Lixia Chen . A selective HK2 degrader suppresses SW480 cancer cell growth by degrading HK2. Chinese Chemical Letters, 2024, 35(8): 109264-. doi: 10.1016/j.cclet.2023.109264
17. [17]
  Xiangqian Cao , Chenkai Yang , Xiaodong Zhu , Mengxin Zhao , Yilin Yan , Zhengnan Huang , Jinming Cai , Jingming Zhuang , Shengzhou Li , Wei Li , Bing Shen . Synergistic enhancement of chemotherapy for bladder cancer by photothermal dual-sensitive nanosystem with gold nanoparticles and PNIPAM. Chinese Chemical Letters, 2024, 35(8): 109199-. doi: 10.1016/j.cclet.2023.109199
18. [18]
  Qiang Li , Jiangbo Fan , Hongkai Mu , Lin Chen , Yongzhen Yang , Shiping Yu . Nucleus-targeting orange-emissive carbon dots delivery adriamycin for enhanced anti-liver cancer therapy. Chinese Chemical Letters, 2024, 35(6): 108947-. doi: 10.1016/j.cclet.2023.108947
19. [19]
  Yihan Zhou , Duo Gao , Yaying Wang , Li Liang , Qingyu Zhang , Wenwen Han , Jie Wang , Chunliu Zhu , Xinxin Zhang , Yong Gan . Worm-like micelles facilitate the intestinal mucus diffusion and drug accumulation for enhancing colorectal cancer therapy. Chinese Chemical Letters, 2024, 35(6): 108967-. doi: 10.1016/j.cclet.2023.108967
20. [20]
  Boran Cheng , Lei Cao , Chen Li , Fang-Yi Huo , Qian-Fang Meng , Ganglin Tong , Xuan Wu , Lin-Lin Bu , Lang Rao , Shubin Wang . Fluorine-doped carbon quantum dots with deep-red emission for hypochlorite determination and cancer cell imaging. Chinese Chemical Letters, 2024, 35(6): 108969-. doi: 10.1016/j.cclet.2023.108969

Get Citation

PDF

XML

Metrics

PDF Downloads(5)
Abstract views(235)
HTML views(28)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142