Citation: Dailu Jia, Wenjiao Fan, Wei Ren, Chenghui Liu. One-step detection of T4 polynucleotide kinase activity based on single particle-confined enzyme reaction and digital particle counting[J]. Chinese Chemical Letters, ;2023, 34(4): 107673. doi: 10.1016/j.cclet.2022.07.016 shu

One-step detection of T4 polynucleotide kinase activity based on single particle-confined enzyme reaction and digital particle counting

Dailu Jia ^{a, b} ,
Wenjiao Fan ^{a, b} ,
Wei Ren ^{a, b} ,
Chenghui Liu ^{a, b, *,}

a.
Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Xi'an 710119, China
b.
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an 710119, China

liuch@snnu.edu.cn

Received Date: 22 April 2022
Revised Date: 22 June 2022
Accepted Date: 8 July 2022
Available Online: 10 July 2022

Figures(4)

T4 polynucleotide kinase (T4 PNK) is a pivotal enzyme for DNA replication, recombination, and DNA damage repair. Herein, a robust single particle counting-based assay has been developed for the high-sensitive determination of T4 PNK activity through only a simple one-step reaction. Taking benefit of the exceptional space-confined enzymatic property of T4 PNK towards DNA substrates on a single nanoparticle, the T4 PNK activity can be precisely determined by counting the fluorescence-positive nanoparticles in a digital manner with a total internal reflection fluorescent microscope (TIRFM). Due to the featured spatial-confined enzymatic property of T4 PNK and the single particle counting-based signal readout, T4 PNK can be effectively differentiated from other interfering enzymes. This facile strategy has been also successfully applied to screen T4 PNK inhibitor and accurately determine T4 PNK activity in complex biological samples, paving a potential avenue for the digital analysis of biomarkers.
- T4 PNK,
- Single particle counting,
- Total internal reflection fluorescent microscope,
- Space-confined enzyme reaction,
- Nanoparticles
1. [1]
  L.K. Wang, C.D. Lima, S. Shuman, EMBO J. 21 (2002) 3873–3880. doi: 10.1093/emboj/cdf397
2. [2]
  N. Tahbaz, S. Subedi, M. Weinfeld, Nucleic Acids Res. 40 (2012) 3484–3495. doi: 10.1093/nar/gkr1245
3. [3]
  J. Shen, E.C. Gilmore, C.A. Marshall, et al., Nat. Genet. 42 (2010) 245–249. doi: 10.1038/ng.526
4. [4]
  J.J. Reynolds, A.K. Walker, E.C. Gilmore, C.A. Walsh, K.W. Caldecott, Nucleic Acids Res. 40 (2012) 6608–6619. doi: 10.1093/nar/gks318
5. [5]
  S. Sharma, K.M. Doherty, R.M. Brosh, Biochem. J. 398 (2006) 319–337. doi: 10.1042/BJ20060450
6. [6]
  S.L. Allinson, Future Oncol. 5 (2010) 1031–1042.
7. [7]
  N.K. Bernstein, R.S. Williams, M.L. Rakovszky, et al., Mol. Cell 17 (2005) 657–670. doi: 10.1016/j.molcel.2005.02.012
8. [8]
  C.J. Whitehouse, R.M. Taylor, A. Thistlethwaite, et al., Cell 104 (2001) 107–117. doi: 10.1016/S0092-8674(01)00195-7
9. [9]
  C. Chappell, L.A. Hanakahi, F. Karimi-Busheri, M. Weinfeld, S.C. West, EMBO J. 21 (2002) 2827–2832. doi: 10.1093/emboj/21.11.2827
10. [10]
  X. Wang, X. Chen, R.H. Zhou, et al., Anal. Chem. 93 (2021) 3889–3897. doi: 10.1021/acs.analchem.0c04562
11. [11]
  C. Feng, Z.H. Wang, T.S. Chen, et al., Anal. Chem. 90 (2018) 2810–2815. doi: 10.1021/acs.analchem.7b04924
12. [12]
  M. Liu, F. Ma, Q.Y. Zhang, C.Y. Zhang, Chem. Commun. 54 (2018) 1583–1586. doi: 10.1039/C7CC09573B
13. [13]
  Y.C. Zhang, C.H. Liu, S.J. Sun, Y.L. Tang, Z.P. Li, Chem. Commun. 51 (2015) 5832–5835. doi: 10.1039/C5CC00572H
14. [14]
  L.J. Wang, Q. Zhang, B. Tang, C.Y. Zhang, Anal. Chem. 89 (2017) 7255–7261. doi: 10.1021/acs.analchem.7b01783
15. [15]
  Y. Li, Q. Liu, L. Cui, et al., Chem. Commun. 57 (2021) 6376–6379. doi: 10.1039/D1CC02035H
16. [16]
  H.X. Jiang, D.M. Kong, H.X. Shen, Biosens. Bioelectron. 55 (2014) 133–138. doi: 10.1016/j.bios.2013.12.001
17. [17]
  C. Jiang, C. Yan, J. Jiang, R. Yu, Anal. Chim. Acta 766 (2013) 88–93. doi: 10.1016/j.aca.2012.12.034
18. [18]
  L. Cui, J. Hu, C.C. Li, C.M. Wang, C.Y. Zhang, Biosens. Bioelectron. 122 (2018) 168–174. doi: 10.1016/j.bios.2018.09.061
19. [19]
  M.H. Lin, H. Wan, J. Zhang, et al., ACS Appl. Mater. Interfaces 12 (2020) 45814–45821. doi: 10.1021/acsami.0c13385
20. [20]
  L. Cui, Y. Li, M. Lu, B. Tang, C.Y. Zhang, Biosens. Bioelectron. 99 (2018) 1–7. doi: 10.1016/j.bios.2017.07.028
21. [21]
  H.Z. He, K.H. Leung, W. Wang, et al., Chem. Commun. 50 (2014) 5313–5315. doi: 10.1039/C3CC47444E
22. [22]
  W. Tang, G. Zhu, C.Y. Zhang, Chem. Commun. 50 (2014) 4733–4735. doi: 10.1039/c4cc00256c
23. [23]
  J. Du, Q. Xu, X. Lu, C.Y. Zhang, Anal. Chem. 86 (2014) 8481–8488. doi: 10.1021/ac502240c
24. [24]
  S. Sakamoto1, T. Komatsu1, R. Watanabe, et al., Sci. Adv. 6 (2020) eaay0888. doi: 10.1126/sciadv.aay0888
25. [25]
  K. Akama, K. Shirai, S. Suzuki, Anal. Chem. 88 (2016) 7123–7129. doi: 10.1021/acs.analchem.6b01148
26. [26]
  L. Cohen, M.R. Hartman, A. Amardey-Wellington, D.R. Walt, Nucleic Acids Res. 45 (2017) e137. doi: 10.1093/nar/gkx542
27. [27]
  W.J. Fan, Y. Qi, X.H. Lu, et al., Chem. Commun. 56 (2020) 7179–7182. doi: 10.1039/D0CC03059G
28. [28]
  Z. Farka, M.J. Mickert, M. Pastucha, et al., Angew. Chem. Int. Ed. 59 (2020) 10746–10773. doi: 10.1002/anie.201913924
29. [29]
  Y.X. Lu, Z.D. Tong, Z.H. Wu, et al., Chin. Chem. Lett. 33 (2022) 3188–3192. doi: 10.1016/j.cclet.2021.12.045
30. [30]
  L. Mou, H.H. Hong, X.J. Xu, Y. Xia, X.Y. Jiang, ACS Nano 15 (2021) 13077–13084. doi: 10.1021/acsnano.1c02311
31. [31]
  T. Tian, B.W. Shu, Y.Z. Jiang, et al., ACS Nano 15 (2021) 1167–1178. doi: 10.1021/acsnano.0c08165
32. [32]
  C. Wu, P.M. Garden, D.R. Walt, J. Am. Chem. Soc. 142 (2020) 12314–12323. doi: 10.1021/jacs.0c04331
33. [33]
  V. Yelleswarapu, J.R. Buser, M. Haber, et al., Proc. Natl. Acad. Sci. U. S. A. 116 (2019) 4489–4495. doi: 10.1073/pnas.1814110116
34. [34]
  L.J. Zhang, W.J. Fan, D.L. Jia, et al., Anal. Chem. 93 (2021) 14828–14836. doi: 10.1021/acs.analchem.1c03724
35. [35]
  K. Subramanian, W. Rutvisuttinunt, W. Scott, R.S. Myers, Nucleic Acids Res. 31 (2003) 1585–1596. doi: 10.1093/nar/gkg266
36. [36]
  Z.W. Tang, K.M. Wang, W.H. Tan, et al., Nucleic Acids Res. 33 (2005) e97. doi: 10.1093/nar/gni096
37. [37]
  C.B. Ma, Z.W. Tang, K.M. Wang, et al., ChemBioChem 8 (2007) 1487–1490. doi: 10.1002/cbic.200700171
38. [38]
  E.A. Galburt, J. Pelletier, G. Wilson, B.L. Stoddard, Structure 10 (2002) 1249–1260. doi: 10.1016/S0969-2126(02)00835-3
1. [1]
  Bohan Chen , Liming Gong , Jing Feng , Mingji Jin , Liqing Chen , Zhonggao Gao , Wei Huang . Research advances of nanoparticles for CAR-T therapy in solid tumors. Chinese Chemical Letters, 2024, 35(9): 109432-. doi: 10.1016/j.cclet.2023.109432
2. [2]
  Yiran Tao , Chunlei Dai , Zhaoxiang Xie , Xinru You , Kaiwen Li , Jun Wu , Hai Huang . Redox responsive polymeric nanoparticles enhance the efficacy of cyclin dependent kinase 7 inhibitor for enhanced treatment of prostate cancer. Chinese Chemical Letters, 2024, 35(8): 109170-. doi: 10.1016/j.cclet.2023.109170
3. [3]
  Yihao Zhang , Yang Jiao , Xianchao Jia , Qiaojia Guo , Chunying Duan . Highly effective self-assembled porphyrin MOCs nanomaterials for enhanced photodynamic therapy in tumor. Chinese Chemical Letters, 2024, 35(5): 108748-. doi: 10.1016/j.cclet.2023.108748
4. [4]
  Yixin Zhang , Ting Wang , Jixiang Zhang , Pengyu Lu , Neng Shi , Liqiang Zhang , Weiran Zhu , Nongyue He . Formation mechanism for stable system of nanoparticle/protein corona and phospholipid membrane. Chinese Chemical Letters, 2024, 35(4): 108619-. doi: 10.1016/j.cclet.2023.108619
5. [5]
  Keyang Li , Yanan Wang , Yatao Xu , Guohua Shi , Sixian Wei , Xue Zhang , Baomei Zhang , Qiang Jia , Huanhua Xu , Liangmin Yu , Jun Wu , Zhiyu He . Flash nanocomplexation (FNC): A new microvolume mixing method for nanomedicine formulation. Chinese Chemical Letters, 2024, 35(10): 109511-. doi: 10.1016/j.cclet.2024.109511
6. [6]
  Yifei Zhang , Yuncong Xue , Laiwei Gao , Rui Liao , Feng Wang , Fei Wang . Merging non-covalent and covalent crosslinking: En route to single chain nanoparticles. Chinese Chemical Letters, 2024, 35(6): 109217-. doi: 10.1016/j.cclet.2023.109217
7. [7]
  Shiqi Peng , Yongfang Rao , Tan Li , Yufei Zhang , Jun-ji Cao , Shuncheng Lee , Yu Huang . Regulating the electronic structure of Ir single atoms by ZrO₂ nanoparticles for enhanced catalytic oxidation of formaldehyde at room temperature. Chinese Chemical Letters, 2024, 35(7): 109219-. doi: 10.1016/j.cclet.2023.109219
8. [8]
  Bei Li , Zhaoke Zheng . In situ monitoring of the spatial distribution of oxygen vacancies at the single-particle level. Chinese Journal of Structural Chemistry, 2024, 43(10): 100331-100331. doi: 10.1016/j.cjsc.2024.100331
9. [9]
  Wangyan Hu , Ke Li , Xiangnan Dou , Ning Li , Xiayan Wang . Nano-sized stationary phase packings retained by single-particle frit for microchip liquid chromatography. Chinese Chemical Letters, 2024, 35(4): 108806-. doi: 10.1016/j.cclet.2023.108806
10. [10]
  Gengchen Guo , Tianyu Zhao , Ruichang Sun , Mingzhe Song , Hongyu Liu , Sen Wang , Jingwen Li , Jingbin Zeng . Au-Fe₃O₄ dumbbell-like nanoparticles based lateral flow immunoassay for colorimetric and photothermal dual-mode detection of SARS-CoV-2 spike protein. Chinese Chemical Letters, 2024, 35(6): 109198-. doi: 10.1016/j.cclet.2023.109198
11. [11]
  Jin Long , Xingqun Zheng , Bin Wang , Chenzhong Wu , Qingmei Wang , Lishan Peng . Improving the electrocatalytic performances of Pt-based catalysts for oxygen reduction reaction via strong interactions with single-CoN₄-rich carbon support. Chinese Chemical Letters, 2024, 35(5): 109354-. doi: 10.1016/j.cclet.2023.109354
12. [12]
  Bharathi Natarajan , Palanisamy Kannan , Longhua Guo . Metallic nanoparticles for visual sensing: Design, mechanism, and application. Chinese Journal of Structural Chemistry, 2024, 43(9): 100349-100349. doi: 10.1016/j.cjsc.2024.100349
13. [13]
  Zhi Li , Wenpei Li , Shaoping Jiang , Chuan Hu , Yuanyu Huang , Maxim Shevtsov , Huile Gao , Shaobo Ruan . Legumain-triggered aggregable gold nanoparticles for enhanced intratumoral retention. Chinese Chemical Letters, 2024, 35(7): 109150-. doi: 10.1016/j.cclet.2023.109150
14. [14]
  Feng Cui , Fangman Chen , Xiaochun Xie , Chenyang Guo , Kai Xiao , Ziping Wu , Yinglu Chen , Junna Lu , Feixia Ruan , Chuanxu Cheng , Chao Yang , Dan Shao . Scalable production of mesoporous titanium nanoparticles through sequential flash nanocomplexation. Chinese Chemical Letters, 2024, 35(4): 108681-. doi: 10.1016/j.cclet.2023.108681
15. [15]
  Xiangyuan Zhao , Jinjin Wang , Jinzhao Kang , Xiaomei Wang , Hong Yu , Cheng-Feng Du . Ni nanoparticles anchoring on vacuum treated Mo2TiC2Tx MXene for enhanced hydrogen evolution activity. Chinese Journal of Structural Chemistry, 2023, 42(10): 100159-100159. doi: 10.1016/j.cjsc.2023.100159
16. [16]
  Xinyi Hu , Riguang Zhang , Zhao Jiang . Depositing the PtNi nanoparticles on niobium oxide to enhance the activity and CO-tolerance for alkaline methanol electrooxidation. Chinese Journal of Structural Chemistry, 2023, 42(11): 100157-100157. doi: 10.1016/j.cjsc.2023.100157
17. [17]
  Xue Xin , Qiming Qu , Islam E. Khalil , Yuting Huang , Mo Wei , Jie Chen , Weina Zhang , Fengwei Huo , Wenjing Liu . Hetero-phase zirconia encapsulated with Au nanoparticles for boosting electrocatalytic nitrogen reduction. Chinese Chemical Letters, 2024, 35(5): 108654-. doi: 10.1016/j.cclet.2023.108654
18. [18]
  Guorong Li , Yijing Wu , Chao Zhong , Yixin Yang , Zian Lin . Predesigned covalent organic framework with sulfur coordination: Anchoring Au nanoparticles for sensitive colorimetric detection of Hg(Ⅱ). Chinese Chemical Letters, 2024, 35(5): 108904-. doi: 10.1016/j.cclet.2023.108904
19. [19]
  Xiangqian Cao , Chenkai Yang , Xiaodong Zhu , Mengxin Zhao , Yilin Yan , Zhengnan Huang , Jinming Cai , Jingming Zhuang , Shengzhou Li , Wei Li , Bing Shen . Synergistic enhancement of chemotherapy for bladder cancer by photothermal dual-sensitive nanosystem with gold nanoparticles and PNIPAM. Chinese Chemical Letters, 2024, 35(8): 109199-. doi: 10.1016/j.cclet.2023.109199
20. [20]
  Lu Li , Suticha Chunta , Xianzi Zheng , Haisheng He , Wei Wu , Yi Lu . β-Lactoglobulin stabilized lipid nanoparticles enhance oral absorption of insulin by slowing down lipolysis. Chinese Chemical Letters, 2024, 35(4): 108662-. doi: 10.1016/j.cclet.2023.108662

Get Citation

PDF

XML

Metrics

PDF Downloads(9)
Abstract views(535)
HTML views(77)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142