Citation: Jingjing Li, Juanjuan Wei, Yixuan Gao, Qi Zhao, Jianghui Sun, Jin Ouyang, Na Na. Peptide-assembled siRNA nanomicelles confine MnO_x-loaded silicages for synergistic chemical and gene-regulated cancer therapy[J]. Chinese Chemical Letters, ;2023, 34(4): 107662. doi: 10.1016/j.cclet.2022.07.005 shu

Peptide-assembled siRNA nanomicelles confine MnO_x-loaded silicages for synergistic chemical and gene-regulated cancer therapy

Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100085, China

nana@bnu.edu.cn

Received Date: 30 March 2022
Revised Date: 16 June 2022
Accepted Date: 6 July 2022
Available Online: 8 July 2022

Figures(5)

Chemodynamic therapy (CDT) is a promising therapeutic approach for in situ cancer treatment, but it is still hindered by inefficient single-modality treatment and the weak targeted delivery of reagents into mitochondria (the main site of intracellular ROS production). Herein, to obtain a multimodal strategy, peptide-assembled siRNA nanomicelles were prepared to confine ultrasmall MnO in small silica cages (silicages), which is convenient for synergistic chemical and gene-regulated cancer therapy. Given the free energy and versatility of small silicages, as well as the excellent Fenton-like activity of ultrasmall MnO, MnO-inside-loaded silicages (10 nm) were prepared for CDT delivery to mitochondria. Subsequently, to obtain a synergistic CDT and gene silencing treatment, the peptide-mediated assembly of siRNA and MnO-loaded silicages were employed to obtain silicage@MnO-siRNA nanomicelles (SMS NMs). After multiple modifications, sequential cancer cell-targeted delivery, GSH-controlled reagent release of siRNA and mitochondria-targeted delivery of MnO-loaded silicages were successfully achieved. Finally, by both in vitro and in vivo experiments, SMS NMs were confirmed to be effective for synergistic chemical and gene-regulated cancer therapy. Our findings expand the applications of silicages and initiate the development of multimodal CDT.
- Peptide-assembled siRNA nanomicelles,
- MnO_x-loaded silicages,
- GSH-stimulated release,
- Mitochondria-targeted delivery,
- Cancer therapy
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

Peptide-assembled siRNA nanomicelles confine MnOx-loaded silicages for synergistic chemical and gene-regulated cancer therapy

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通讯作者: 陈斌, bchen63@163.com

Peptide-assembled siRNA nanomicelles confine MnO_x-loaded silicages for synergistic chemical and gene-regulated cancer therapy