Citation: Xiang Shi, Ge Gao, Xiaoyang Liu, Lingling Xu, Yu Deng, Rui Wang, Gaolin Liang. Highly lipophilic coumarin fluorophore with excimer-monomer transition property for lipid droplet imaging[J]. Chinese Chemical Letters, ;2023, 34(3): 107613. doi: 10.1016/j.cclet.2022.06.036 shu

Highly lipophilic coumarin fluorophore with excimer-monomer transition property for lipid droplet imaging

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China

ruiwang938@seu.edu.cn

gliang@seu.edu.cn

Received Date: 4 March 2022
Revised Date: 13 June 2022
Accepted Date: 14 June 2022
Available Online: 18 June 2022

Figures(5)

Lipid droplet (LD) fluorescent imaging plays an important role in the detection of lipid-related diseases. Due to their poor photostability and low hydrophobicity of currently available LD imaging fluorophores, LD imaging is limited by its short imaging period and low imaging contrast. Herein, we reasonably designed a highly lipophilic compound Cou-Flu with excellent photostability and excimer-monomer transition property. It exhibited weak excimer emission in cytoplasm, but strong monomer emission in LDs, enabling high contrast LD imaging and LD movement tracing in cells. Zebrafish imaging study demonstrated that Cou-Flu was also suitable for in vivo LD detection with excellent sensitivity. We anticipate that Cou-Flu could be widely applied to understand LD-related intracellular activities and even LD-related diseases in the future.
- Excimer-monomer transition,
- Fluorescent probe,
- Hydrophobicity,
- Lipid droplets,
- Monomer emission
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