Citation: Houyu Han, Jingyu Cui, Dianming Zhou, Deping Hua, Weipan Peng, Mengyao Lin, Yating Zhang, Fangya Li, Xiaoqun Gong, Jianyu Zhang. Single-stranded RNA as primers of terminal deoxynucleotidyl transferase for template-independent DNA polymerization[J]. Chinese Chemical Letters, ;2023, 34(2): 107533. doi: 10.1016/j.cclet.2022.05.047 shu

Single-stranded RNA as primers of terminal deoxynucleotidyl transferase for template-independent DNA polymerization

Houyu Han ^a ,
Jingyu Cui ^a ,
Dianming Zhou ^{c, d} ,
Deping Hua ^a ,
Weipan Peng ^a ,
Mengyao Lin ^a ,
Yating Zhang ^a ,
Fangya Li ^b ,
Xiaoqun Gong ^{a, *,} ,
Jianyu Zhang ^{b, *,}

a.
School of Life Sciences, Tianjin University and Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology (Tianjin), Tianjin 300072, China
b.
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China
c.
Tianjin Key Laboratory of Pathogenic Microbiology of Infectious Disease, Tianjin Centers for Disease Control and Prevention, Tianjin 300011, China
d.
Department of Toxicology, Tianjin Centers for Disease Control and Prevention, Tianjin 300011, China

gongxiaoqun@tju.edu.cn

jianyu.zhang@tju.edu.cn

Received Date: 26 February 2022
Revised Date: 7 May 2022
Accepted Date: 15 May 2022
Available Online: 19 May 2022

Figures(4)

Terminal deoxynucleotidyl transferase (TdT) has been characterized as template-independent polymerase using single-stranded DNA (ssDNA) as primers to generate random oligonucleotides. However, the extension performance of TdT to single-stranded RNA (ssRNA) is vague. By systematically comparing and contrasting the performance of TdT-catalyzed ssDNA and ssRNA extension, it is indicated that the catalytic efficiency of ssRNA as primers was about 3 times lower than ssDNA as primers. Collectively, it is believed that understanding the catalytic performance of TdT will help to design the strategy to synthesize chimeric DNA on 3′-OH of ssRNA, which becomes invaluable.
- Terminal deoxynucleotidyl transferase,
- Single-stranded RNA,
- Single-stranded DNA,
- Template-independent,
- Polymerization
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