Citation: Binfen Zhao, Wenhui Xu, Jiutong Ma, Qiong Jia. Design and fabrication of highly hydrophilic magnetic material by anchoring L-cysteine onto chitosan for efficient enrichment of glycopeptides[J]. Chinese Chemical Letters, ;2023, 34(3): 107498. doi: 10.1016/j.cclet.2022.05.012 shu

Design and fabrication of highly hydrophilic magnetic material by anchoring L-cysteine onto chitosan for efficient enrichment of glycopeptides

Binfen Zhao ^a ,
Wenhui Xu ^a ,
Jiutong Ma ^a ,
Qiong Jia ^{a, b, *,}

a.
College of Chemistry, Jilin University, Changchun 130012, China
b.
Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China

jiaqiong@jlu.edu.cn

Received Date: 18 January 2022
Revised Date: 12 April 2022
Accepted Date: 7 May 2022
Available Online: 11 May 2022

Figures(4)

Hydrophilic interaction liquid chromatography (HILIC) has been recognized as an effective strategy for glycopeptide enrichment. Hydrophilic materials pave the way to solve the limit of low enrichment capacity and poor selectivity. The present study is the first attempt to combine chitosan (CS) and L-cysteine (L-Cys) to design a novel hydrophilic material focusing on glycopeptide enrichment. CS containing a large number of hydrophilic amino and hydroxyl groups has unique chemical properties, which makes it a very attractive biomaterial for glycopeptide enrichment. The excellent hydrophilicity of zwitterionic molecule L-Cys inspires the idea of anchoring L-Cys onto CS to design a novel hydrophilic material (named as Fe₃O₄@CS@Au-L-Cys) for the capture of low abundance glycopeptides. To be specific, Au nanoparticles (Au NPs) was introduced into CS-coated Fe₃O₄ via electrostatic interaction and served as bridges to anchor L-Cys onto the surface of CS through strong Au-S bond interaction. The prepared Fe₃O₄@CS@Au-L-Cys exhibited strong affinity, low detection limit (0.5 fmol/µL HRP), high selectivity (HRP/BSA with a molar ratio of 1:1000) for glycopeptides. Moreover, successful application of glycopeptide enrichment in human serum and saliva by Fe₃O₄@CS@Au-L-Cys was achieved. A satisfactory data set indicates that Fe₃O₄@CS@Au-L-Cys has promising potential in the application of glycopeptide enrichment in real complex bio-samples and for related glycoproteome research.
- Chitosan,
- L-cysteine,
- Hydrophilic material,
- Glycopeptide,
- Enrichment
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