Citation: Yaxin Cui, Tianyu Zhu, Xueyan Zhang, Jicong Chen, Fengying Sun, Youxin Li, Lesheng Teng. Oral delivery of superoxide dismutase by lipid polymer hybrid nanoparticles for the treatment of ulcerative colitis[J]. Chinese Chemical Letters, ;2022, 33(10): 4617-4622. doi: 10.1016/j.cclet.2022.03.077 shu

Oral delivery of superoxide dismutase by lipid polymer hybrid nanoparticles for the treatment of ulcerative colitis

School of Life Sciences, Jilin University, Changchun 130012, China

liyouxin@jlu.edu.cn

tenglesheng@jlu.edu.cn

Received Date: 11 November 2021
Revised Date: 12 March 2022
Accepted Date: 16 March 2022
Available Online: 19 March 2022

Figures(4)

Protein-based drugs have received extensive attention in the field of drug research in recent years. However, protein-based drug activity is difficult to maintain during oral delivery, which limits its application. This study developed bifunctional oral lipid polymer hybrid nanoparticles (R8-PEG-PPNPs) that deliver superoxide dismutase (SOD) for the treatment of ulcerative colitis (UC). R8-PEG-PPNPs was composed of PCADK, PLGA, lecithin, and co-modified with stearic acid-octa-arginine and polyethylene glycol. The nanoparticles (NPs) are uniformly dispersed with a complete spherical structure. In vitro stability and release studies showed that R8-PEG-PPNPs exhibited good stability and protection. In vitro cell culture experiments demonstrated that R8-PEG-PPNPs as carriers have no significant toxic effects on cells at concentration below 1000 µg/mL and promote cellular uptake. In experiments with ulcerative colitis mice, R8-PEG- PPNPs were able to enhance drug absorption by intestinal epithelial cells and accumulate effectively at the site of inflammation. Its therapeutic effect further demonstrates that R8-PEG-PPNPs are a promising delivery system for oral delivery of protein-based drugs.
- Superoxide dismutase,
- PCADK DSPE-PEG,
- Cell-penetrating peptide,
- Ulcerative colitis,
- Lipid polymer hybrid nanoparticles
1. [1]
  S. Khafagy el, M. Morishita, Y. Onuki, K. Takayama, Adv. Drug Deliv. Rev.59 (2007) 1521-1546. doi: 10.1016/j.addr.2007.08.019
2. [2]
  L.M. Ensign, R. Cone, J. Hanes, Adv. Drug Deliv. Rev. 64 (2012) 557-570. doi: 10.1016/j.addr.2011.12.009
3. [3]
  A.A. Date, J. Hanes, L.M. Ensign, J. Control. Release 240 (2016) 504-526. doi: 10.1016/j.jconrel.2016.06.016
4. [4]
  N. Marasini, M. Skwarczynski, I. Toth, Expert Rev. Vaccines 13 (2014) 1361-1376. doi: 10.1586/14760584.2014.936852
5. [5]
  J. Sheng, H. He, L. Han, et al., J. Control. Release 233 (2016) 181-190. doi: 10.1016/j.jconrel.2016.05.015
6. [6]
  M. Yang, S.K. Lai, Y.Y. Wang, et al., Angew. Chem. Int. Ed. 50 (2011) 2597-2600. doi: 10.1002/anie.201006849
7. [7]
  S.S. Lucky, N.M. Idris, K. Huang, et al., Theranostics 6 (2016) 1844-1865. doi: 10.7150/thno.15088
8. [8]
  J. Xie, J. Huang, X. Li, S. Sun, X. Chen, Curr. Med. Chem. 16 (2009) 1278-1294. doi: 10.2174/092986709787846604
9. [9]
  J. Xie, G. Liu, H.S. Eden, H. Ai, X. Chen, Acc. Chem. Res. 44 (2011) 883-892. doi: 10.1021/ar200044b
10. [10]
  H.B. Ruttala, N. Chitrapriya, K. Kaliraj, et al., Acta Biomater. 63 (2017) 135-149. doi: 10.1016/j.actbio.2017.09.002
11. [11]
  H.T. Shi, X.Y. Zhao, J.S. Gao, et al., Chin. Chem. Lett. 31 (2020) 3102-3106. doi: 10.1016/j.cclet.2020.03.039
12. [12]
  L. Zhang, J.M. Chan, F.X. Gu, et al., ACS Nano 2 (2008) 1696-1702. doi: 10.1021/nn800275r
13. [13]
  S.N. Zhao, J.H. Li, F.Z. Wang, et al., Chin. Chem. Lett. 31 (2020) 1147-1152. doi: 10.1109/ispec50848.2020.9351076
14. [14]
  M. Zhang, D. Merlin, Inflamm. Bowel Dis. 24 (2018) 1401-1415. doi: 10.1093/ibd/izy123
15. [15]
  A.T. Florence, J. Drug Target. 12 (2004) 65-70. doi: 10.1080/10611860410001693706
16. [16]
  B. Xiao, Z. Zhang, E. Viennois, et al., Theranostics 6 (2016) 2250-2266. doi: 10.7150/thno.15710
17. [17]
  X. Pu, N. Ye, M. Lin, et al., Carbohyd. Polym. 273 (2021) 118612. doi: 10.1016/j.carbpol.2021.118612
18. [18]
  W. Shan, X. Zhu, M. Liu, et al., ACS Nano 9 (2015) 2345-2356. doi: 10.1021/acsnano.5b00028
19. [19]
  M. Liu, J. Zhang, X. Zhu, et al., J. Control. Release 222 (2016) 67-77. doi: 10.1016/j.jconrel.2015.12.008
20. [20]
  X. Zhu, J. Wu, W. Shan, et al., Adv. Funct. Mater. 26 (2016) 2728-2738. doi: 10.1002/adfm.201505000
21. [21]
  C. He, Y. Hu, L. Yin, C. Tang, C. Yin, Biomaterials 31 (2010) 3657-3666. doi: 10.1016/j.biomaterials.2010.01.065
22. [22]
  X. Murgia, P. Pawelzyk, U.F. Schaefer, et al., Biomacromolecules 17 (2016) 1536-1542. doi: 10.1021/acs.biomac.6b00164
23. [23]
  K. Maisel, L. Ensign, M. Reddy, R. Cone, J. Hanes, J. Control. Release 197 (2015) 48-57. doi: 10.1016/j.jconrel.2014.10.026
24. [24]
  J. Sheng, L. Han, J. Qin, et al., ACS Appl. Mater. Inter. 7 (2015) 15430-15441. doi: 10.1021/acsami.5b03555
25. [25]
  A. Verma, F. Stellacci, Small 6 (2010) 12-21. doi: 10.1002/smll.200901158
26. [26]
  E. Oh, J.B. Delehanty, K.E. Sapsford, et al., ACS Nano 5 (2011) 6434-6448. doi: 10.1021/nn201624c
27. [27]
  E. Böhmová, D. Machová, M. Pechar, et al., Physiol. Res. 67 (2018) S267-S279. doi: 10.33549/physiolres.933975
28. [28]
  Y. Chen, L. Yuan, L. Zhou, et al., Int. J. Nanomed. 7 (2012) 4581-4591.
29. [29]
  A. Falanga, L. Lombardi, E. Galdiero, V.D. Genio, S. Galdiero, Future Med. Chem. 12 (2020) 1431-1446. doi: 10.4155/fmc-2020-0140
30. [30]
  S.C. Yang, M. Bhide, I.N. Crispe, R.H. Pierce, N. Murthy, Bioconjug. Chem. 19 (2008) 1164-1169. doi: 10.1021/bc700442g
31. [31]
  F. Danhier, E. Ansorena, J.M. Silva, et al., J. Control. Release 161 (2012) 505-522. doi: 10.1016/j.jconrel.2012.01.043
32. [32]
  G. Hartmann, C. Bidlingmaier, B. Siegmund, et al., J. Pharmacol. Exp. Ther. 292 (2000) 22-30.
33. [33]
  H. Zhou, Y. Ikeuchi-Takahashi, Y. Hattori, H. Onishi, Int. J. Mol. Sci. 21 (2020) 2376. doi: 10.3390/ijms21072376
34. [34]
  D. Wang, M. Sun, Y. Zhang, et al., Phytomedicine 78 (2020) 153293. doi: 10.1016/j.phymed.2020.153293
35. [35]
  J. Xu, M. Tam, S. Samaei, et al., Acta Biomater. 48 (2017) 247-257. doi: 10.1016/j.actbio.2016.10.026
36. [36]
  M. Naeem, M.A. Oshi, J. Kim, et al., Nanomedicine. 14 (2018) 823-834. doi: 10.1016/j.nano.2018.01.003
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