Citation: Cunli Wang, Xue Wang, Dongdong Wang, Shengxu Qian, Fusheng Zhang, Mingyang Li, Minmin Li, Wenqi Lu, Bo Liu, Guangyan Qing. Remarkable difference of phospholipid molecular chirality in regulating PrP aggregation and cell responses[J]. Chinese Chemical Letters, ;2023, 34(2): 107332. doi: 10.1016/j.cclet.2022.03.055 shu

Remarkable difference of phospholipid molecular chirality in regulating PrP aggregation and cell responses

Cunli Wang ^{a, b, 1} ,
Xue Wang ^{b, 1} ,
Dongdong Wang ^b ,
Shengxu Qian ^b ,
Fusheng Zhang ^b ,
Mingyang Li ^b ,
Minmin Li ^b ,
Wenqi Lu ^b ,
Bo Liu ^b ,
Guangyan Qing ^{b, c, *,}

a.
School of Biomedical Engineering, Dalian University of Technology, Dalian 116024, China
b.
CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
c.
College of Chemistry and Chemical Engineering, Wuhan Textile University, Wuhan 430200, China

qinggy@dicp.ac.cn

Received Date: 25 October 2021
Revised Date: 31 January 2022
Accepted Date: 14 March 2022
Available Online: 17 March 2022

Figures(8)

Prion diseases are fatal neurodegenerative diseases that can cause severe dementia. The misfolding and accumulation of the prion peptide (PrP)_106–126 is crucial, and this process is closely relevant to biological membranes. However, how PrP_106–126 aggregation is affected by the molecular chirality of phospholipid membrane is unknown. Thus, in this study, a pair of L- and D-aspartic acid (Asp)-modified 1, 2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE) were synthesized to construct chiral liposomes. We discover that L-Asp-DPPE liposomes strongly inhibit the oligomerization and amyloidogenesis of PrP_106–126, whether acting on monomers or oligomers, which rescues cytotoxicity induced by PrP_106–126. By comparison, D-Asp-DPPE liposomes inhibit peptide oligomerization only at a high concentration and cannot prevent amyloidogenesis when acting on oligomers, which lead to pronounced cytotoxicity. Apoptosis experiment, dynamic change of intracellular Ca²⁺ (_iCa²⁺) and Ca²⁺ release from endoplasmic reticulum (ER), reactive oxygen species (ROS) production, adsorption dynamics and affinity tests, and fluorescent imaging clearly disclose that molecular chirality of the liposomes dominates conformational transition of PrP_106–126 from random coil to β-sheet, binding and adsorption of the monomers and oligomers, and subsequent fibrillation process, resulting in distinct inhibition effect in Ca²⁺ overload and release, ROS production and cell apoptosis. This work is the first to report that interfacial molecular chirality is a potentially crucial influence on the fibrillation process of PrP_106–126 and its cell responses, whereas the convergence of chiral amino acids and liposomes can be considered potential inhibitors in prion diseases.
- Prion disease,
- Liposome,
- Chirality,
- Interface,
- Cytosolic Ca²⁺
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