Citation: Wenhao Wang, Huihui Liu, Zhengwei Huang, Fangqin Fu, Wenhua Wang, Linjing Wu, Ying Huang, Chuanbin Wu, Xin Pan. The effect of organic ligand modification on protein corona formation of nanoscale metal organic frameworks[J]. Chinese Chemical Letters, ;2022, 33(9): 4185-4190. doi: 10.1016/j.cclet.2022.02.052 shu

The effect of organic ligand modification on protein corona formation of nanoscale metal organic frameworks

Wenhao Wang ^{a, 1} ,
Huihui Liu ^{a, 1} ,
Zhengwei Huang ^{b, *,} ,
Fangqin Fu ^b ,
Wenhua Wang ^a ,
Linjing Wu ^b ,
Ying Huang ^{b, *,} ,
Chuanbin Wu ^b ,
Xin Pan ^{a, *,}

a.
School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China
b.
College of Pharmacy, Jinan University, Guangzhou 511443, China

huangzhengw@jnu.edu.cn

huangy2007@jnu.edu.cn

panxin2@mail.sysu.edu.cn

Received Date: 12 November 2021
Revised Date: 11 February 2022
Accepted Date: 18 February 2022
Available Online: 22 February 2022

Figures(4)

Nanoscale metal organic frameworks (NMOFs) have been widely reported in biomedical field for their unique porous structure and tunable multifunctionality. However, when administrated in vivo, the protein corona will be formed on the surface of NMOFs, significantly affecting their biodistribution, pharmacokinetics and drug release. Few studies paid attention to the protein corona formation process and its influencing factors of NMOFs. As a well-established strategy for altering structure features of NMOFs, the organic ligand modification may have effect on the protein corona formation process, which is to be investigated. In this study, the zirconium (Zr)-based UIO66 was chosen as model NMOFs, the organic ligand of which was modified with amino group (-NH₂) or carboxyl group (-COOH) to synthesize UIO66-NH₂ and UIO66-2COOH, respectively. Bovine serum albumin (BSA) was chosen as model protein to investigate the protein corona formation process of NMOFs. The current results showed that the -COOH modification remarkably enhanced the BSA adsorption on NMOFs while -NH₂ slightly decreased the protein binding affinity. These differences may be ascribed to the two different dominate protein corona formation modes, i.e., surface coating mode and porous embedded mode. The protein corona formation did not affect the crystal phase of NMOFs but increased the content of α-helix of BSA. Ultimately, upon protein corona formation, the cellular uptake of NMOFs was significantly affected. We believe our study will provide a new research paradigm to the design and applications of NMOFs.
- Protein corona,
- NMOFs,
- UIO66,
- Organic ligand modification,
- BSA
1. [1]
  W. Poon, B.R. Kingston, B. Ouyang, et al., Nat. Nanotechnol. 15 (2020) 819–829. doi: 10.1038/s41565-020-0759-5
2. [2]
  S. Li, M. Li, S. Huo, et al., Adv. Mater. 33 (2021) 1–10.
3. [3]
  M. Chen, D. Yang, Y. Sun, et al., ACS Nano 15 (2021) 3387–3401. doi: 10.1021/acsnano.0c10396
4. [4]
  G. Erel-Akbaba, L.A. Carvalho, T. Tian, et al., ACS Nano 13 (2019) 4028–4040. doi: 10.1021/acsnano.8b08177
5. [5]
  B. Li, T. Chu, J. Wei, et al., Nano Lett. 21 (2021) 2588–2595. doi: 10.1021/acs.nanolett.1c00168
6. [6]
  B. Wu, J. Fu, Y. Zhou, et al., Acta Pharm. Sin. B 10 (2020) 2198–2211. doi: 10.1016/j.apsb.2020.07.025
7. [7]
  M. Cao, R. Cai, L. Zhao, et al., Nat. Nanotechnol. 16 (2021) 708–716. doi: 10.1038/s41565-021-00856-w
8. [8]
  R. Cai, C. Chen, Adv. Mater. 31 (2019) 1–13.
9. [9]
  I. Lynch, A. Salvati, K.A. Dawson, Nat. Nanotechnol. 4 (2009) 546–547. doi: 10.1038/nnano.2009.248
10. [10]
  Y. Yan, K.T. Gause, M.M.J. Kamphuis, et al., ACS Nano 7 (2013) 10960–10970. doi: 10.1021/nn404481f
11. [11]
  L. Wang, S.Y. Li, W. Jiang, et al., ACS Appl. Mater. Interfaces 12 (2020) 32312–32320. doi: 10.1021/acsami.0c07016
12. [12]
  B.E. Givens, E. Wilson, J. Fiegel, Colloids Surf. B: Biointerfaces 179 (2019) 374–381. doi: 10.1016/j.colsurfb.2019.04.012
13. [13]
  L. Guo, T. Wang, Z. Chen, et al., Chin. Chem. Lett. 29 (2018) 1291–1295. doi: 10.1016/j.cclet.2017.11.017
14. [14]
  G. Wang, W. Wang, E. Shangguan, S. Gao, Y. Liu, Mater. Sci. Eng. C 111 (2020) 110830. doi: 10.1016/j.msec.2020.110830
15. [15]
  W. Wang, Z. Huang, Y. Li, et al., Acta Pharm. Sin. B 11 (2021) 1030–1046. doi: 10.1016/j.apsb.2020.10.023
16. [16]
  M.X. Wu, Y.W. Yang, Adv. Mater. 29 (2017) 1–20.
17. [17]
  Q.Y. Xu, Z. Tan, X.W. Liao, C. Wang, Chin. Chem. Lett. 33 (2021) 22–32.
18. [18]
  T. Wen, G. Quan, B. Niu, et al., Small. 17 (2021) 2005064. doi: 10.1002/smll.202005064
19. [19]
  E. Ploetz, A. Zimpel, V. Cauda, et al., Adv. Mater. 32 (2020) 1907267. doi: 10.1002/adma.201907267
20. [20]
  N. Gan, Q. Sun, L. Zhao, et al., Int. J. Biol. Macromol. 140 (2019) 709–718. doi: 10.1016/j.ijbiomac.2019.08.183
21. [21]
  S. Jafari, Z. Izadi, L. Alaei, et al., Sci. Rep. 10 (2020) 1–14. doi: 10.1038/s41598-019-56847-4
22. [22]
  S. Rojas, F.J. Carmona, C.R. Maldonado, et al., Inorg. Chem. 55 (2016) 2650–2663. doi: 10.1021/acs.inorgchem.6b00045
23. [23]
  J.S. Kahn, L. Freage, N. Enkin, M.A.A. Garcia, I. Willner, Adv. Mater. 29 (2017) 1–6. doi: 10.1002/adma.201602782
24. [24]
  Z. Fan, H. Liu, Y. Xue, et al., Bioact. Mater. 6 (2021) 312–325. doi: 10.1016/j.bioactmat.2020.08.005
25. [25]
  P. Gao, R. Lou, X. Liu, et al., Anal. Chem. 93 (2021) 5437–5441. doi: 10.1021/acs.analchem.0c05060
26. [26]
  J.H. Cavka, S. Jakobsen, U. Olsbye, et al., J. Am. Chem. Soc. 130 (2008) 13850–13851. doi: 10.1021/ja8057953
27. [27]
  L.L. Tan, H. Li, Y. Zhou, et al., Small. 11 (2015) 3807–3813. doi: 10.1002/smll.201500155
28. [28]
  P. Zhang, Y. Ouyang, Y.S. Sohn, et al., ACS Nano 15 (2021) 6645–6657. doi: 10.1021/acsnano.0c09996
29. [29]
  I. Abánades Lázaro, C.J.R. Wells, R.S. Forgan, Angew. Chem. Int. Ed. 59 (2020) 5211–5217. doi: 10.1002/anie.201915848
30. [30]
  J.H. Cavka, S. Jakobsen, U. Olsbye, et al., J. Am. Chem. Soc. 130 (2008) 13850–13851. doi: 10.1021/ja8057953
31. [31]
  Y. Wang, Y. Qiu, A. Sun, et al., Anal. Chim. Acta 1133 (2020) 109–118. doi: 10.22323/1.390.0109
32. [32]
  G. Wang, C. Yan, S. Gao, Y. Liu, Mater. Sci. Eng. C 103 (2019) 109856. doi: 10.1016/j.msec.2019.109856
33. [33]
  O. Vilanova, J.J. Mittag, P.M. Kelly, et al., ACS Nano 10 (2016) 10842–10850. doi: 10.1021/acsnano.6b04858
34. [34]
  F. Fu, Z. Huang, W. Wang, et al., J. Drug Deliv. Sci. Technol. 64 (2021) 102376. doi: 10.1016/j.jddst.2021.102376
35. [35]
  S. Dominguez-Medina, L. Kisley, L.J. Tauzin, et al., ACS Nano 10 (2016) 2103–2112. doi: 10.1021/acsnano.5b06439
36. [36]
  R. del Caño, L. Mateus, G. Sánchez-Obrero, et al., J. Colloid Interface Sci. 505 (2017) 1165–1171. doi: 10.1016/j.jcis.2017.07.011
37. [37]
  Q. Peng, S. Zhang, Q. Yang, et al., Biomaterials 34 (2013) 8521–8530. doi: 10.1016/j.biomaterials.2013.07.102
1. [1]
  Yixin Zhang , Ting Wang , Jixiang Zhang , Pengyu Lu , Neng Shi , Liqiang Zhang , Weiran Zhu , Nongyue He . Formation mechanism for stable system of nanoparticle/protein corona and phospholipid membrane. Chinese Chemical Letters, 2024, 35(4): 108619-. doi: 10.1016/j.cclet.2023.108619
2. [2]
  Ruowen Liang , Chao Zhang , Guiyang Yan . Enhancing CO2 cycloaddition through ligand functionalization: A case study of UiO-66 metal-organic frameworks. Chinese Journal of Structural Chemistry, 2024, 43(2): 100211-100211. doi: 10.1016/j.cjsc.2023.100211
3. [3]
  Wenhao Wang , Siyuan Peng , Zhengwei Huang , Xin Pan . Tuning amino/hydroxyl ratios of nanovesicles to manipulate protein corona-mediated in vivo fate. Chinese Chemical Letters, 2024, 35(11): 110134-. doi: 10.1016/j.cclet.2024.110134
4. [4]
  Wenbi Wu , Yinchu Dong , Haofan Liu , Xuebing Jiang , Li Li , Yi Zhang , Maling Gou . Modification of plasma protein for bioprinting via photopolymerization. Chinese Chemical Letters, 2024, 35(8): 109260-. doi: 10.1016/j.cclet.2023.109260
5. [5]
  Zhanggui DUAN , Yi PEI , Shanshan ZHENG , Zhaoyang WANG , Yongguang WANG , Junjie WANG , Yang HU , Chunxin LÜ , Wei ZHONG . Preparation of UiO-66-NH₂ supported copper catalyst and its catalytic activity on alcohol oxidation. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 496-506. doi: 10.11862/CJIC.20230317
6. [6]
  Yu-Hang Li , Shuai Gao , Lu Zhang , Hanchun Chen , Chong-Chen Wang , Haodong Ji . Insights on selective Pb adsorption via O 2p orbit in UiO-66 containing rich-zirconium vacancies. Chinese Chemical Letters, 2024, 35(8): 109894-. doi: 10.1016/j.cclet.2024.109894
7. [7]
  Ruolin CHENG , Haoran WANG , Jing REN , Yingying MA , Huagen LIANG . Efficient photocatalytic CO₂ cycloaddition over W₁₈O₄₉/NH₂-UiO-66 composite catalyst. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 523-532. doi: 10.11862/CJIC.20230349
8. [8]
  Yuan Zhang , Shenghao Gong , A.R. Mahammed Shaheer , Rong Cao , Tianfu Liu . Plasmon-enhanced photocatalytic oxidative coupling of amines in the air using a delicate Ag nanowire@NH₂-UiO-66 core-shell nanostructures. Chinese Chemical Letters, 2024, 35(4): 108587-. doi: 10.1016/j.cclet.2023.108587
9. [9]
  Yangrui Xu , Yewei Ren , Xinlin Liu , Hongping Li , Ziyang Lu . 具有高传质和亲和表面的NH₂-UIO-66基疏水多孔液体用于增强CO₂光还原. Acta Physico-Chimica Sinica, 2024, 40(11): 2403032-. doi: 10.3866/PKU.WHXB202403032
10. [10]
  Mingqi Wang , Shixin Fa , Jiate Yu , Guoxian Zhang , Yi Yan , Qing Liu , Qiuyu Zhang . Light-controlled protein imprinted nanospheres with variable recognition specificity. Chinese Chemical Letters, 2025, 36(2): 110124-. doi: 10.1016/j.cclet.2024.110124
11. [11]
  Jie ZHANG , Xin LIU , Zhixin LI , Yuting PEI , Yuqi YANG , Huimin LI , Zhiqiang LIU . Assembling a luminescence silencing system based on post-synthetic modification strategy: A highly sensitive and selective turn-on metal-organic framework probe for ascorbic acid detection. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 823-833. doi: 10.11862/CJIC.20230310
12. [12]
  Ying Li , Long-Jie Wang , Yong-Kang Zhou , Jun Liang , Bin Xiao , Ji-Shen Zheng . An improved installation of 2-hydroxy-4-methoxybenzyl (iHmb) method for chemical protein synthesis. Chinese Chemical Letters, 2024, 35(5): 109033-. doi: 10.1016/j.cclet.2023.109033
13. [13]
  Si Ha , Jiacheng Zhu , Hua Xiang , Guoshun Luo . Hydrophobic tag tethering degrader as a promising paradigm of protein degradation: Past, present and future perspectives. Chinese Chemical Letters, 2024, 35(8): 109192-. doi: 10.1016/j.cclet.2023.109192
14. [14]
  Bo Liu , Shuaiqiang Shao , Junjie Cai , Zijian Zhang , Feng Tian , Kun Yang , Fan Li . Signal cascade amplification of streptavidin-biotin-modified immunofluorescence nanocapsules for ultrasensitive detection of glial fibrillary acidic protein. Chinese Chemical Letters, 2025, 36(3): 109814-. doi: 10.1016/j.cclet.2024.109814
15. [15]
  Han-Min Wang , Yan-Chen Li , Lu-Lu Sun , Ming-Ye Tang , Jia Liu , Jiahao Cai , Lei Dong , Jia Li , Yi Zang , Hai-Hao Han , Xiao-Peng He . Protein-encapsulated long-wavelength fluorescent probe hybrid for imaging lipid droplets in living cells and mice with non-alcoholic fatty liver. Chinese Chemical Letters, 2024, 35(11): 109603-. doi: 10.1016/j.cclet.2024.109603
16. [16]
  Gengchen Guo , Tianyu Zhao , Ruichang Sun , Mingzhe Song , Hongyu Liu , Sen Wang , Jingwen Li , Jingbin Zeng . Au-Fe₃O₄ dumbbell-like nanoparticles based lateral flow immunoassay for colorimetric and photothermal dual-mode detection of SARS-CoV-2 spike protein. Chinese Chemical Letters, 2024, 35(6): 109198-. doi: 10.1016/j.cclet.2023.109198
17. [17]
  Kongchuan Wu , Dandan Lu , Jianbin Lin , Ting-Bin Wen , Wei Hao , Kai Tan , Hui-Jun Zhang . Elucidating ligand effects in rhodium(Ⅲ)-catalyzed arene–alkene coupling reactions. Chinese Chemical Letters, 2024, 35(5): 108906-. doi: 10.1016/j.cclet.2023.108906
18. [18]
  Yuanjin Chen , Xianghui Shi , Dajiang Huang , Junnian Wei , Zhenfeng Xi . Synthesis and reactivity of cobalt dinitrogen complex supported by nonsymmetrical pincer ligand. Chinese Chemical Letters, 2024, 35(7): 109292-. doi: 10.1016/j.cclet.2023.109292
19. [19]
  Luyao Lu , Chen Zhu , Fei Li , Pu Wang , Xi Kang , Yong Pei , Manzhou Zhu . Ligand effects on geometric structures and catalytic activities of atomically precise copper nanoclusters. Chinese Journal of Structural Chemistry, 2024, 43(10): 100411-100411. doi: 10.1016/j.cjsc.2024.100411
20. [20]
  Lijun Mao , Shuo Li , Xin Zhang , Zhan-Ting Li , Da Ma . Cucurbit[n]uril-based nanostructure construction and modification. Chinese Chemical Letters, 2024, 35(8): 109363-. doi: 10.1016/j.cclet.2023.109363

Get Citation

PDF

XML

Metrics

PDF Downloads(7)
Abstract views(642)
HTML views(74)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142