Citation: Zhukang Guo, Baijiang Jin, Yile Fang, Yan Deng, Zhu Chen, Hui Chen, Song Li, Franklin Wang-Ngan Chow, Polly H.M. Leung, Hanming Wang, Lei Cai, Nongyue He. Selected aptamer specially combing 5-8F cells based on automatic screening instrument[J]. Chinese Chemical Letters, ;2022, 33(9): 4208-4212. doi: 10.1016/j.cclet.2022.01.081 shu

Selected aptamer specially combing 5-8F cells based on automatic screening instrument

Zhukang Guo ^a ,
Baijiang Jin ^a ,
Yile Fang ^a ,
Yan Deng ^b ,
Zhu Chen ^b ,
Hui Chen ^b ,
Song Li ^b ,
Franklin Wang-Ngan Chow ^c ,
Polly H.M. Leung ^c ,
Hanming Wang ^d ,
Lei Cai ^e ,
Nongyue He ^{a, b, *,}

a.
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
b.
Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China
c.
Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hong Kong, China
d.
Guangzhou Wondfo Biotech Co., Ltd., Guangzhou 510641, China
e.
Guangzhou Wondfo iCubate Biotech Co., Ltd., Guangzhou 510641, China

nyhe1958@163.com

Received Date: 7 January 2022
Revised Date: 26 January 2022
Accepted Date: 27 January 2022
Available Online: 3 February 2022

Figures(4)

Since the concept of aptamer emerged, many scientists have launched a rich field of research around it. However, few nucleic acids aptamer which use cell as target can be put into practical applications. We believe that a great deal of this lies in the complexity and irreproducibility of aptamer screening experiments themselves. The complexity is due to the cumbersome processes and the technical requirements for laboratory personnel, whereas irreproducibility arises from the fact that the starting point of such screens is nucleic acid libraries with random fragments, and that different libraries directly determine the differences or even the success or failure of screening results. The complexity and irreproducibility mentioned above, in turn, lead to the inability of this experiment to unfold on a large scale, which naturally cannot lead to excellent results for practical applications. In response to this problem, our group has developed an instrument for automated screening of tumor cell nucleic acid aptamers and characterized the properties of nucleic acid aptamers obtained using this instrument in a comprehensive manner.
- Aptamer,
- Cell-SELEX,
- Automatic instrument,
- Tumor,
- Efficient screening
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