Citation: Liming Zhang, Lingfeng Xu, Yi Wang, Jieyu Liu, Guanghong Tan, Fengying Huang, Nongyue He, Zhuoxuan Lu. A novel therapeutic vaccine based on graphene oxide nanocomposite for tumor immunotherapy[J]. Chinese Chemical Letters, ;2022, 33(8): 4089-4095. doi: 10.1016/j.cclet.2022.01.071 shu

A novel therapeutic vaccine based on graphene oxide nanocomposite for tumor immunotherapy

Liming Zhang ^{a, 1} ,
Lingfeng Xu ^{a, c, 1} ,
Yi Wang ^a ,
Jieyu Liu ^a ,
Guanghong Tan ^a ,
Fengying Huang ^a ,
Nongyue He ^{b, *,} ,
Zhuoxuan Lu ^{a, *,}

a.
Key Laboratory of Tropical Translational Medicine of Ministry of Education & Hainan Provincial Key Laboratory of Tropical Medicine, Hainan Medical University, Haikou 571199, China
b.
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
c.
Department of Clinical Laboratory, HwaMei Hospital, University of Chinese Academy of Sciences, Ningbo 315000, China

nyhe1958@163.com

Luzhuoxuan1981@163.com

Received Date: 25 October 2021
Revised Date: 17 January 2022
Accepted Date: 26 January 2022
Available Online: 1 February 2022

Figures(6)

With an intensive understanding of the mechanism of immune system, developing a therapeutic tumor vaccine is one of the most perspective strategy of cancer immunotherapy. In this study, we report a facile approach to prepare graphene oxide (GO)-based therapeutic cancer-nanovaccine. The model antigen (ovalbumin, OVA) and adjuvant (CpG ODN), are conjugated with GO-PEI nanosheet through electrostatic interaction. The addition of PEG can improve biocompatibility and prevent nanoparticle aggregation. The prepared GO-based nanovaccine, GO-PEI-OVA-PEG-CpG, exhibits good biocompatibility and low toxicity both in vivo and in vitro. More importantly, it can efficiently induce the maturation of dendritic cells (DCs), the enhancement of antigen cross-presentation ability, and the amplification of cytokine production of immune cells. Impressively, this nanovaccine shows a remarkable therapeutic effect against pre-established B16-OVA-melanoma tumors, which can significantly inhibit tumor growth and prolong the survival time of the OVA-expressed tumor-bearing mice. Moreover, combining GO-PEI-OVA-PEG-CpG with NLG919, an IDO-1 (indoleamine-2,3-dioxygenase) inhibitor which can regulate the tumor microenvironment, displays a synergistic therapeutic effect. These findings indicate the GO-PEI-OVA-PEG-CpG nanovaccine actively induces an antigen-specific antitumor immune response and it combined with NLG919 could achieve better therapeutic outcomes.
- Nanovaccine,
- Graphene Oxide,
- Adjuvant,
- Tumor-specific antigen,
- NLG919
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