Citation: Yujun Ning, Lin Wei, Shen Lin, Yifan Jiang, Naidong Wang, Lehui Xiao. Dissection the endocytic routes of viral capsid proteins-coated upconversion nanoparticles by single-particle tracking[J]. Chinese Chemical Letters, ;2022, 33(10): 4710-4714. doi: 10.1016/j.cclet.2021.12.084 shu

Dissection the endocytic routes of viral capsid proteins-coated upconversion nanoparticles by single-particle tracking

Yujun Ning ^{a, b} ,
Lin Wei ^c ,
Shen Lin ^b ,
Yifan Jiang ^{d, e} ,
Naidong Wang ^{d, *,} ,
Lehui Xiao ^{b, *,}

a.
College of Chemistry, Zhengzhou University, Zhengzhou 450001, China
b.
State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, College of Chemistry, Nankai University, Tianjin 300071, China
c.
College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China
d.
College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China
e.
School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China

naidongwang@hunau.edu.cn

lehuixiao@nankai.edu.cn

Received Date: 24 September 2021
Revised Date: 26 October 2021
Accepted Date: 30 December 2021
Available Online: 4 January 2022

Figures(4)

Real-time exploring the cellular endocytic pathway of viral capsid proteins (VCPs) functionalized nanocargos at the single-particle level can provide deep insight into the kinetic information involved in virus infection. In this work, porcine circovirus type 2 (PCV2) VCPs with different functions are modified onto the surface of upconversion nanoparticles (VCPs-UCNPs) to investigate the cellular internalization process in real-time. Clathrin-mediated endocytosis is found to be the essential uptake mechanism for these VCPs-UCNPs. Besides, it is verified that P₁-UCNPs (PCV2 VCPs with nuclear localization signal, namely P₁) can be easily assembled close to the perinuclear area, which is different from that of P₂-UCNPs (PCV2 VCPs without nuclear localization signal, namely P₂). Interestingly, multistep entry processes are observed. Particularly, confined diffusion is observed during the transmembrane process. The intracellular transport of VCPs-UCNPs is dependent on microtubules toward the cell interior. During this process, P₁-UCNPs display increased velocities with active transport, while diffusion much faster around the perinuclear area. But for P₂-UCNPs, there are only two phases involved in their endocytosis process. This study presents distinct dynamic mechanisms for the nanocargos with different functions, which would make a useful contribution to the development of robust drug delivery systems.
- Upconversion nanoparticles,
- Viral capsid proteins,
- Single-particle tracking,
- Hepatoma cells,
- Endocytosis
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