Citation: Jun Liao, Bo Pan, Xiaobin Zhuo, Guochao Liao, Yun Gao, Zhenzhen Yao, Lianghua Wang, Qiuye Wu, Weihua Pan, Binghua Jiao, Qingjie Zhao. β-1, 2-Mannan-based glycoconjugates as potential antifungal vaccines[J]. Chinese Chemical Letters, ;2022, 33(9): 4345-4349. doi: 10.1016/j.cclet.2021.12.065 shu

β-1, 2-Mannan-based glycoconjugates as potential antifungal vaccines

Jun Liao ^{a, b, 1} ,
Bo Pan ^{c, 1} ,
Xiaobin Zhuo ^{b, 1} ,
Guochao Liao ^d ,
Yun Gao ^a ,
Zhenzhen Yao ^a ,
Lianghua Wang ^a ,
Qiuye Wu ^b ,
Weihua Pan ^{c, *,} ,
Binghua Jiao ^{a, *,} ,
Qingjie Zhao ^{b, *,}

a.
Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China
b.
School of Pharmacy, Second Military Medical University, Shanghai 200433, China
c.
Department of Dermatology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
d.
International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China

panweihua@smmu.edu.cn

bhjiao@smmu.edu.cn

qjzhao@smmu.edu.cn

Received Date: 16 September 2021
Revised Date: 19 December 2021
Accepted Date: 22 December 2021
Available Online: 29 December 2021

Figures(7)

Phosphorylated di-, tri- and tetra-saccharides of β-1, 2-mannan antigen derived from Candida albicans (C. albicans) cell wall were synthesized and covalently conjugated with keyhole limpet hemocyanin (KLH) and human serum albumin (HSA) via a bifunctional linker under mild conditions. The semi-synthetic β-1, 2-mannoside–KLH conjugates were evaluated for the immunization of BALB/c mice. The ELISA results revealed that all three conjugates could elicit high levels of specific IgG antibodies and the acquired antisera could effectively identify the β-1, 2-mannan epitope. Furthermore, the immunofluorescence and flow cytometry assays also uncovered that the induced antibodies, especially that obtained from immunization with β-1, 2-mannotriose–KLH conjugate (1b), could bind well to fungi cell. Eventually, the structure–immunogenicity relationship analysis of β-mannan showed that the length of oligo-β-mannoses had a big impact on their immunogenicity and β-1, 2-mannotriose showed the strongest immunogenicity. The results suggested the great potential of β-1, 2-mannotriose–KLH conjugate as an antifungal vaccine candidate.
- β-1, 2-Mannan,
- Oligosaccharide,
- Antifungal,
- Glycoconjugate,
- Vaccine,
- Antigen
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