Citation: Min Hou, Dinggeng He, Huizhen Wang, Jin Huang, Hong Cheng, Kejin Wan, Hung-Wing Li, Zifeng Tang, Xiaoxiao He, Kemin Wang. Simultaneous and multiplex detection of exosomal microRNAs based on the asymmetric Au@Au@Ag probes with enhanced Raman signal[J]. Chinese Chemical Letters, ;2022, 33(6): 3183-3187. doi: 10.1016/j.cclet.2021.11.092 shu

Simultaneous and multiplex detection of exosomal microRNAs based on the asymmetric Au@Au@Ag probes with enhanced Raman signal

Min Hou ^a ,
Dinggeng He ^{a, b, *} ,
Huizhen Wang ^a ,
Jin Huang ^a ,
Hong Cheng ^a ,
Kejin Wan ^a ,
Hung-Wing Li ^b ,
Zifeng Tang ^c ,
Xiaoxiao He ^{a, *} ,
Kemin Wang ^{a, *}

a.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China
b.
Department of Chemistry, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
c.
College of Art and Science, New York University, New York 10012, United States

hedinggeng@hnu.edu.cn

xiaoxiaohe@hnu.edu.cn

kmwang@hnu.edu.cn

Received Date: 24 August 2021
Revised Date: 25 October 2021
Accepted Date: 30 November 2021
Available Online: 4 December 2021

Figures(7)

Simultaneous and quantitative detection of multiple exosomal microRNAs (miRNAs) was successfully performed by a surface-enhanced Raman scattering (SERS) assay consisting of Raman probes and capture probes. In this design, the asymmetric core-shell structured Au@Au@Ag nanoparticles were first synthesized by layer-by-layer self-assembly method and modified with different Raman molecules and recognition sequences (polyA-DNA) to prepare the surface-enhanced Raman probes. Then, the streptavidin-modified magnetic beads were used to immobilize the biotinylated DNA capture sequences (biotin-DNA) to obtain capture probes. In the presence of target exosomal miRNAs, the Raman probes and capture probes could bind to the target exosomal miRNAs in the partial hybridization manner. Thus, the developed SERS sensor could indicate the target miRNAs levels in the buffer solution. Using breast cancer-related miRNAs as model targets, the limits of detection of this sensor were determined to be 1.076 fmol/L for synthetic miR-21, 0.068 fmol/L for synthetic miR-126, and 4.57 fmol/L for synthetic miR-1246, respectively. Such SERS sensors were further employed to detect the miR-21 in 20% human serum and the extraction solution of exosomes, respectively. Therefore, simultaneous and multiplex detection of cancer-related exosomal miRNAs by this assay could provide new opportunities for further biomedical applications.
- SERS,
- Au@Au@Ag probes,
- Exosomes,
- miR-21,
- miR-126,
- miR-1246
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