Citation: Tian Tian, Yuhan Ho, Chen Chen, Hebin Sun, Janan Hui, Panhui Yang, Yuqing Ge, Ting Liu, Jian Yang, Hongju Mao. A 3D bio-printed spheroids based perfusion in vitro liver on chip for drug toxicity assays[J]. Chinese Chemical Letters, ;2022, 33(6): 3167-3171. doi: 10.1016/j.cclet.2021.11.029 shu

A 3D bio-printed spheroids based perfusion in vitro liver on chip for drug toxicity assays

Tian Tian ^{a, b} ,
Yuhan Ho ^c ,
Chen Chen ^d ,
Hebin Sun ^a ,
Janan Hui ^a ,
Panhui Yang ^{a, b} ,
Yuqing Ge ^a ,
Ting Liu ^a ,
Jian Yang ^{e, f} ,
Hongju Mao ^{a, b, *}

a.
Institute of Microsystem and Information Technology (SIMIT), Chinese Academy of Sciences (CAS), Shanghai 200050, China
b.
Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing 100049, China
c.
Shanghai Industrial Technology Research Institute (SITRI), Shanghai 201800, China
d.
School of Stomatology, Dalian Medical University, Dalian 116044, China
e.
State Key Laboratory of component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
f.
Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China

hjmao@mail.sim.ac.cn

Received Date: 10 July 2021
Revised Date: 3 October 2021
Accepted Date: 9 November 2021
Available Online: 13 November 2021

Figures(5)

Liver is the foremost organ of human being for drug metabolism, and it played a significant role in toxicity evaluation of drugs. Establishing a liver model in vitro can accelerate the process of the drug screening and new drug research and development. We provide a 3D printing based hepatic sinusoid-on-a-chip microdevice that reconstitutes organ-level liver functions to create a drug screening model of toxicity evaluation on chip. The microfluidic device, which recapitulates the hepatic sinusoid microenvironment, consists of PET polyporous membranes which mimic the perisinusoidal space, and experience fluid flow to mimic the hepatic arterial capillaries. The PET membrane was used to separate the hepatocyte and endotheliocyte. The endotheliocyte was cultured on the downside of the membrane and the hepatocyte were 3D seeded on the membrane via the 3D printer. This device was used to reproduce the in vitro liver model for drug toxicity assays. The expression of several biomarkers of liver was compared with the monoculture and 2D cultured conditions, and the results reveal that this organ-on-a-chip microdevice mimics the drug hepatoxicity that has not been possible by 2D cell-based and animal models, providing a useful platform for screening the drugs and developing an effective therapy in hepatopathy.
- Liver-on-chip,
- Drug screening,
- Drug toxicity,
- 3D-printing,
- 3D-culture
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