Citation: Yuan Liu, Taotao Li, Gaojian Yang, Yan Deng, Xianbo Mou, Nongyue He. A simple AuNPs-based colorimetric aptasensor for chlorpyrifos detection[J]. Chinese Chemical Letters, ;2022, 33(4): 1913-1916. doi: 10.1016/j.cclet.2021.11.025 shu

A simple AuNPs-based colorimetric aptasensor for chlorpyrifos detection

Yuan Liu ^a ,
Taotao Li ^b ,
Gaojian Yang ^a ,
Yan Deng ^{c, *,} ,
Xianbo Mou ^d ,
Nongyue He ^{a, c, **,}

a.
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
b.
Hunan Provincial Key Lab of Dark Tea and Jin-Hua, School of Materials and Chemical Engineering, Hunan City University, Yiyang 413000, China
c.
Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China
d.
Medical School of Ningbo University, Ningbo 315211, China

^**

hndengyan@126.com

nyhe1958@163.com

Received Date: 11 July 2021
Revised Date: 3 November 2021
Accepted Date: 4 November 2021
Available Online: 11 November 2021

Figures(4)

In this work, a simple gold nanoparticles (AuNPs) based colorimetric biosensor was developed for chlorpyrifos (Chl) detection using an aptamer as the capture probe. The Chl-aptamer with low dissociation constant (K_d) of 58.59 ± 6.08 nmol/L was selected by ssDNA library immobilized systematic evolution of ligands by enrichment (SELEX). In the absence of Chl, the Chl-aptamer acted as the stabilizer for AuNPs in salt solution. In the presence of Chl, the highly specific Chl-aptamer bound with Chl targets immediately, thus a self-aggregation of AuNPs induced by salt was displayed. The fabricated colorimetric aptasensor exhibited an excellent sensitivity for Chl detection with the LOD as low as 14.46 nmol/L. In addition, the aptasensor was applied to test Chl in tap water, cucumber and cabbage samples, the excellent recoveries with acceptable RSD values below 5% demonstrated that the method can be considered as a promising tool for simple, rapid Chl detection.
- Aptamer,
- Colorimetric aptasensor,
- SELEX,
- Chlorpyrifos,
- AuNPs
1. [1]
  S.Y. Foong, N.L. Ma, S.S. Lam, et al., J. Hazard. Mater. 400 (2020) 123006. doi: 10.1016/j.jhazmat.2020.123006
2. [2]
  X. Huang, H. Cui, W. Duan, Ecotox. Environ. Safa. 200 (2020) 110731. doi: 10.1016/j.ecoenv.2020.110731
3. [3]
  A.P. Mestre, P.S. Amavet, I.S. Sloot, et al., Chemosphere 252 (2020) 126433. doi: 10.1016/j.chemosphere.2020.126433
4. [4]
  X. Zhang, W. Cui, K. Wang, et al., Environ. Res. 188 (2020) 109785. doi: 10.1016/j.envres.2020.109785
5. [5]
  S.W. Todd, E.W. Lumsden, Y. Aracava, et al., Neuropharmacology 180 (2020) 108271. doi: 10.1016/j.neuropharm.2020.108271
6. [6]
  M.Y. Xu, P. Wang, Y.J. Sun, et al., Chem. Res. Toxicol. 32 (2019) 122-129. doi: 10.1021/acs.chemrestox.8b00219
7. [7]
  M. Lasagna, M.S. Hielpos, C. Ventura, et al., Ecotox. Environ. Safa. 205 (2020) 111312. doi: 10.1016/j.ecoenv.2020.111312
8. [8]
  P. Moyano, J. Garcia, J.M. Garcia, et al., Chemosphere 251 (2020) 126426. doi: 10.1016/j.chemosphere.2020.126426
9. [9]
  J. Zhang, L. Liu, L. Ren, et al., J. Hazard. Mater. 334 (2017) 121-131. doi: 10.1016/j.jhazmat.2017.03.055
10. [10]
  L. He, X. Luo, H. Xie, et al., Anal. Chim. Acta 655 (2009) 52-59. doi: 10.1016/j.aca.2009.09.044
11. [11]
  F. Darvishnejad, J.B. Raoof, M. Ghani, Anal. Chim. Acta 1140 (2020) 99-110. doi: 10.1016/j.aca.2020.10.015
12. [12]
  P. Balsini, H. Parastar, J. Sep. Sci. 42 (2019) 3553-3562. doi: 10.1002/jssc.201900823
13. [13]
  Y. Lee, Y.J. Kim, M.S.I. Khan, et al., J. Sep. Sci. 42 (2020) 4047-4057.
14. [14]
  C. Zhai, T.F. Xu, Y.K. Peng, et al., Spectrosc. Spectr. Anal. 36 (2016) 2835-2840.
15. [15]
  L. Fan, G. Zhao, H. Shi, M. Liu, Z. Li, Biosens. Bioelectron. 43 (2013) 12-18. doi: 10.1016/j.bios.2012.11.033
16. [16]
  H. Wang, H. Cheng, J. Wang, et al., Talanta 154 (2016) 498-503. doi: 10.1016/j.talanta.2016.04.005
17. [17]
  A. Sinha, P. Gopinathan, Y.D. Chung, et al., Biosens. Bioelectron. 122 (2018) 104-112. doi: 10.1016/j.bios.2018.09.040
18. [18]
  Y. Luo, J. Wang, L. Yang, et al., Sens. Actuators B: Chem. 276 (2018) 128-135. doi: 10.1016/j.snb.2018.08.105
19. [19]
  R. Huang, L. He, S. Li, et al., Nanoscale 12 (2020) 2445-2451. doi: 10.1039/C9NR08747H
20. [20]
  Z.K. Guo, Y. Liu, N.Y. He, et al., Chin. Chem. Lett. 32 (2021) 40-47. doi: 10.1016/j.cclet.2020.11.061
21. [21]
  Z. Xi, R. Huang, Z. Li, et al., ACS Appl. Mater. Inter. 7 (2015) 11215-11223. doi: 10.1021/acsami.5b01180
22. [22]
  M. Liu, Z. Yang, B. Li, et al., Food Chem. 339 (2021) 128059. doi: 10.1016/j.foodchem.2020.128059
23. [23]
  H. Li, S. Xing, J. Xu, et al., Talanta 221 (2021) 121670. doi: 10.1016/j.talanta.2020.121670
24. [24]
  M. Liu, L. Xi, T. Tan, et al., Chin. Chem. Lett. 32 (2021) 1726-1730. doi: 10.1016/j.cclet.2020.11.072
25. [25]
  R.R. Huang, L. He, Y.Y. Xia, et al., Small 15 (2019) 1900735. doi: 10.1002/smll.201900735
26. [26]
  Z. Guo, C. Wang, S. Li, et al., J. Nanosci. Nanotechnol. 20 (2020) 3373-3377. doi: 10.1166/jnn.2020.17424
27. [27]
  T. Li, J. Yang, Z. Ali, et al., Sci. China Chem. 60 (2017) 370-376. doi: 10.1007/s11426-016-0159-2
28. [28]
  J. Pan, Q. Li, D. Zhou, et al., Talanta 189 (2018) 370-376. doi: 10.1016/j.talanta.2018.07.024
29. [29]
  Y. Liu, Y.X. Lai, G.J. Yang, et al., J. Biomed. Nanotechnol. 13 (2017) 1253-1259. doi: 10.1166/jbn.2017.2424
30. [30]
  S. Chen, X. Yang, S. Fu, et al., Food Control 115 (2020) 107281. doi: 10.1016/j.foodcont.2020.107281
31. [31]
  M. Jiang, C. Chen, J. He, et al., Food Chem. 307 (2020) 125534. doi: 10.1016/j.foodchem.2019.125534
32. [32]
  Y. Qi, Y. Chen, F. Xiu, et al., Sens. Actuators B: Chem. 304 (2020) 127359. doi: 10.1016/j.snb.2019.127359
33. [33]
  H. Shen, W. Deng, Y. He, et al., Biosens. Bioelectron. 168 (2020) 112516. doi: 10.1016/j.bios.2020.112516
34. [34]
  Y. Yang, Y. Yin, X. Li, et al., Sens. Actuators B: Chem. 319 (2020) 128250. doi: 10.1016/j.snb.2020.128250
35. [35]
  Q. Ma, Y. Wang, J. Jia, et al., Food Chem. 249 (2018) 98-103. doi: 10.1016/j.foodchem.2018.01.022
36. [36]
  N. Duan, S. Qi, Y. Guo, et al., LWT Food Sci. Technol. 134 (2020) 110017. doi: 10.1016/j.lwt.2020.110017
37. [37]
  M. Liu, A. Khan, Z. Wang, et al., Biosens. Bioelectron. 130 (2019) 174-184. doi: 10.1016/j.bios.2019.01.006
38. [38]
  N. Duan, S. Wu, X. Chen, et al., J. Agric. Food Chem. 61 (2013) 3229-3234. doi: 10.1021/jf400767d
39. [39]
  J. Yi, P. Wu, G. Li, et al., Microchim. Acta 186 (2019) 711. doi: 10.1007/s00604-019-3827-5
40. [40]
  L. He, R.R. Huang, P.F. Xiao, et al., Chin. Chem. Lett. 32 (2021) 1593-1602. doi: 10.1016/j.cclet.2020.12.054
41. [41]
  H. Shi, G. Zhao, M. Liu, et al., J. Hazard. Mater. 260 (2013) 754-761. doi: 10.1016/j.jhazmat.2013.06.031
42. [42]
  A.N. Berlina, A.V. Zherdev, S.M. Pridvorova, et al., J. Nanosci. Nanotechnol. 19 (2020) 5489-5495.
43. [43]
  K. Abnous, N.M. Danesh, M. Ramezani, et al., Microchim. Acta 185 (2018) 216. doi: 10.1007/s00604-018-2752-3
44. [44]
  J. Jia, S. Yan, X. Lai, et al., Food Anal. Methods 11 (2018) 1668-1676. doi: 10.1007/s12161-017-1144-3
45. [45]
  Y. Liu, T. Li, C. Ling, et al., Chin. Chem. Lett. 30 (2019) 2359-2362. doi: 10.1016/j.cclet.2019.10.033
46. [46]
  C. Xu, Y. Ying, J. Ping, Microchim. Acta 186 (2019) 448. doi: 10.1007/s00604-019-3574-7
47. [47]
  M. Qi, C. Tu, Y. Dai, et al., Anal. Methods 10 (2018) 3402-3407. doi: 10.1039/C8AY00713F
48. [48]
  L. Qiao, H. Wang, J. He, et al., Food Chem. 340 (2021) 128181. doi: 10.1016/j.foodchem.2020.128181
49. [49]
  Y. Liu, G.J. Yang, T.T. Li, et al., Chin. Chem. Lett. 32 (2021) 1957-1962. doi: 10.1016/j.cclet.2021.01.016
50. [50]
  G. Frens, Nat. Phys. Sci. 241 (1973) 20-22. doi: 10.1038/physci241020a0
51. [51]
  Y.S. Kim, J.H. Kim, I.A. Kim, et al., Biosens. Bioelectron. 26 (2010) 1644-1649. doi: 10.1016/j.bios.2010.08.046
52. [52]
  K. Sato, K. Hosokawa, M. Maeda, J. Am. Chem. Soc. 125 (2003) 8102-8103. doi: 10.1021/ja034876s
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