Citation: Mengying Liang, Delu Liu, Yangyang Nie, Yanli Liu, Xiaoqiang Qiao. Exploiting styrene-maleic acid copolymer grafting chromatographic stationary phase materials for separation of membrane lipids[J]. Chinese Chemical Letters, ;2022, 33(6): 3123-3126. doi: 10.1016/j.cclet.2021.10.008 shu

Exploiting styrene-maleic acid copolymer grafting chromatographic stationary phase materials for separation of membrane lipids

College of Pharmaceutical Sciences, Hebei Province Key Laboratory of Analytical Science & Technology, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University, Baoding 071002, China

xiaoqiao@hbu.edu.cn

Received Date: 8 July 2021
Revised Date: 12 September 2021
Accepted Date: 8 October 2021
Available Online: 14 October 2021

Figures(5)

High performance liquid chromatography-mass spectrometry is one of the most commonly used strategies for lipid analysis. The development of versatile chromatographic stationary phases to meet the increasing demands for separation of complex lipids is very important. Styrene-maleic acid (SMA) copolymer is an amphiphilic polymer, which has been proven to have the ability to solubilize lipid molecules of various structures. In this study, styrene-maleic anhydride copolymer coated silica was first prepared by the thiol-ene click reaction. With l-cysteine hydrochloride or dodecanol as the post-modification reagents, Sil-SMA-amino acid and Sil-SMA-dodecanol stationary phase materials were further successfully fabricated via nucleophilic ring-opening reaction. The Fourier-transform infrared, thermogravimetric analysis, and elemental analysis results confirmed the two stationary phase materials were successfully prepared. Furthermore, both the Sil-SMA-dodecanol column and the Sil-SMA-amino acid column possessed reversed-phase/hydrophilic interaction/ion exchange mixed-mode retention mechanisms. The column efficiency of the Sil-SMA-derivatives columns reached 77,300 N/m. Based on the mixed-mode retention characteristics, the Sil-SMA-derivatives columns achieved both the lipid classes and species separation via a single column. The Sil-SMA-amino acid column was further successfully used to separate lipid extract from gastric cancer cell membrane. All these results demonstrated that the SMA-based stationary phase materials have a good potential for use in lipid separation.
- Styrene-maleic acid copolymer,
- Mixed-mode,
- Stationary phase,
- Chromatographic separation,
- Lipids
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