Citation: Chenlong Wang, Wanting Hu, Liandi Guan, Xiaoping Yang, Qionglin Liang. Single-cell metabolite analysis on a microfluidic chip[J]. Chinese Chemical Letters, ;2022, 33(6): 2883-2892. doi: 10.1016/j.cclet.2021.10.006 shu

Single-cell metabolite analysis on a microfluidic chip

Chenlong Wang ^{a, 1} ,
Wanting Hu ^{a, b, 1} ,
Liandi Guan ^a ,
Xiaoping Yang ^a ,
Qionglin Liang ^{a, *,}

a.
Center for Synthetic and Systems Biology, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, China
b.
State Key Laboratory of Chemical Oncogenomics, The Graduate School, Tsinghua University, Shenzhen 518055, China

liangql@tsinghua.edu.cn

Received Date: 28 May 2021
Revised Date: 16 September 2021
Accepted Date: 6 October 2021
Available Online: 13 October 2021

Figures(5)

Metabolites can directly reflect and modulate cell responses and phenotypical changes by influencing energy balances, intercellular signals, and many other cellular functions throughout the lifespan of cells. Taking into account the heterogeneity of cells, single-cell metabolite analysis offers an insight into the functional process within one cell. Microfluidics as a powerful tool has attracted significant interest in the single-cell metabolite analysis field. The microfluidic platform is possible to observe, classify, and stimulate individual cells. It can also transport single-cell to subsequent analysis steps in a fast and controllable way to determine and analyze the composition and content of metabolites. The reviews of topics in microfluidics for single-cell metabolite analysis have been published in the past few years. However, most of them focused on metabolite analysis with mass spectrometry. Here, we covered the advances of microfluidic devices for single-cell metabolite analysis, with a focus on single-cell isolation and manipulation. What is more, we summarized the detection methods and applications of single-cell metabolites.
- Single-cell,
- Metabolomics,
- Microfluidic,
- Cell trapping,
- Cell manipulation,
- Detection
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沈阳化工大学材料科学与工程学院沈阳 110142