Citation: Yuqiang Hu, Zhen Zhang, Wei Zhang, Minghao Hu, Xianjin Xiao, Tongbo Wu. Probing and modulating the interactions of the DNAzyme with DNA-functionalized nanoparticles[J]. Chinese Chemical Letters, ;2022, 33(6): 3026-3030. doi: 10.1016/j.cclet.2021.09.039 shu

Probing and modulating the interactions of the DNAzyme with DNA-functionalized nanoparticles

Yuqiang Hu ^a ,
Zhen Zhang ^a ,
Wei Zhang ^{b, c} ,
Minghao Hu ^a ,
Xianjin Xiao ^b ,
Tongbo Wu ^{a, *,}

a.
School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
b.
Institute of Reproductive Health/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
c.
Department of Obstetrics and Gynaecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China

wutongbo@hust.edu.cn

Received Date: 14 July 2021
Revised Date: 11 August 2021
Accepted Date: 9 September 2021
Available Online: 15 September 2021

Figures(4)

DNA-functionalized gold nanoparticles are one of the most versatile bionanomaterials for biomedical and clinical diagnosis. Herein, we discovered that the performance of DNAzyme cleaving the substrate is highly related to its length. This intriguing phenomenon only appears at the interfaces of DNA-functionalized gold nanoparticles. We systematically investigated the causes of this phenomenon. We conjectured that the DNAzyme with extended nucleotides that do not match its substrate strand is vulnerable to non-specific adsorption, electrostatic repulsion, and steric hindrance. Based on our improved understanding of this phenomenon, we have successfully developed a highly sensitive and specific amplifiable biosensor to detect human apurinic/apyrimidinic endonuclease 1.
- Gold nanoparticles,
- DNAzyme,
- Interface interaction,
- Rate difference,
- Human apurinic/apyrimidinic endonuclease 1
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