Citation: Ningning Zhao, Zhiqiang Liu, Junpeng Xing, Zhong Zheng, Fengrui Song, Shu Liu. A novel strategy for high-specificity, high-sensitivity, and high-throughput study for gut microbiome metabolism of aromatic carboxylic acids[J]. Chinese Chemical Letters, ;2022, 33(6): 3031-3034. doi: 10.1016/j.cclet.2021.09.022 shu

A novel strategy for high-specificity, high-sensitivity, and high-throughput study for gut microbiome metabolism of aromatic carboxylic acids

Ningning Zhao ^{a, b} ,
Zhiqiang Liu ^{a, b, c} ,
Junpeng Xing ^a ,
Zhong Zheng ^a ,
Fengrui Song ^a ,
Shu Liu ^{a, *,}

a.
National Center of Mass Spectrometry in Changchun and Jilin Provincial Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
b.
Institute of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei 230029, China
c.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

mslab20@ciac.ac.cn

1985liushu@163.com

Received Date: 16 April 2021
Revised Date: 10 August 2021
Accepted Date: 6 September 2021
Available Online: 10 September 2021

Figures(4)

Aromatic carboxylic acids (ACAs) may be as transformed key metabolites via gut microbiome for playing better pharmacological effects. However, it's rare to achieve high-specificity, high-sensitivity, and high-throughput detection simultaneously, especially, for tracing trace ACAs in gut microbiome. In this work, firstly, a novel dual-template and double-shelled molecularly imprinted 96-well microplates (DDMIPs) was designed and amplified signal for p-hydroxybenzoic acid (PBA) and 3, 4, 5-trimethoxycinnamic acid (TMA). Additionally, the DDMIPs and a stable isotope labeling derivatization (SILD) method combined with the ultra-high performance liquid chromatography triple quadrupole tandem mass spectrometry (UHPLC-TQ MS) was firstly stepwise integrated, achieving high-effective, high-sensitive, and high-throughput study of gut microbiome metabolism. The whole strategy showed lower limits of detections (LODs) up to 1000 folds than the traditional method, and revealed a more real metabolism-time profile of PBA and TMA by 3-step signal amplification. The platform also laid the foundation for fast, simple, high-selective, high-effective, and high-throughput metabolism and pharmacological research.
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