Citation: Yongxin Liu, Mengda Cao, Zhongxi Huang, Changmin Yu, Naidi Yang, Qiong Wu, Liang Shi, Wenjie Duan, Yi Zhu, Jifu Wei, Lin Li, Wei Huang. Ultrasensitive detection of IgE levels based on magnetic nanocapturer linked immunosensor assay for early diagnosis of cancer[J]. Chinese Chemical Letters, ;2022, 33(4): 1855-1860. doi: 10.1016/j.cclet.2021.08.117 shu

Ultrasensitive detection of IgE levels based on magnetic nanocapturer linked immunosensor assay for early diagnosis of cancer

Yongxin Liu ^{a, 1} ,
Mengda Cao ^{c, 1} ,
Zhongxi Huang ^{a, 1} ,
Changmin Yu ^{a, d, **,} ,
Naidi Yang ^a ,
Qiong Wu ^a ,
Liang Shi ^b ,
Wenjie Duan ^b ,
Yi Zhu ^f ,
Jifu Wei ^{b, *,} ,
Lin Li ^{a, *,} ,
Wei Huang ^e

a.
Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech), Nanjing 211816, China
b.
Research Division of Clinical Pharmacology & Clinical Allergy Center, the First Affiliated Hospital of Nanjing Medical University; Department of Pharmacy, Jiangsu Institute of Cancer Research & the Affiliated Cancer Hospital of Nanjing Medical University, Nanjing 210009, China
c.
Department of Pharmacy, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210044, China
d.
State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210023, China
e.
Shaanxi Institute of Flexible Electronics (SIFE), Northwestern Polytechnical University (NPU), Xi'an 710072, China
f.
The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, China

^**

iamcmyu@njtech.edu.cn

weijifu@hotmail.com

iamlli@njtech.edu.cn

Received Date: 25 June 2021
Revised Date: 24 August 2021
Accepted Date: 27 August 2021
Available Online: 3 September 2021

Figures(6)

Rapid and accurate detection of immunoglobulin E (IgE) in serum and reduction of serum dosage are of great significance for clinical detection. Herein, we described a rapid magnetic separation of IgE from patient serum based on Fe₃O₄@SiO₂-NTA@026 sdab as the capture probe and multiple horseradish peroxidase (HRP)-labeled antibodies linked gold nanoparticles (AuNPs) as chemiluminescence (CL) signal amplifier for ultrasensitive detection of total IgE. Results showed that the limit of detection of our immunosensor system in serum samples was 0.03 kU/L, which is lowest in comparison with current methods, and far lower than that of ImmunoCAP for IgE detection (0.1 kU/L). Furthermore, our immunosensor possessed satisfied repeatability and accuracy, as well as good stability. In comparison with the ImmunoCAP for the quantitative detection of IgE, highly consistent results were achieved in 20 serum samples. Specially, this method was also successfully utilized for assessing the IgE traces in breast cancer patients, which provides a new idea for the diagnosis of early cancer. Therefore, we believe that such versatile immunosensor will offer an alternative method for the on-site monitoring and determination of various IgE-related diseases.
- Gold nanoparticles,
- Magnetic nanocapturer,
- IgE detection,
- Ultrasensitive immunosensor,
- Cancer
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