Citation: Guangyue Zou, Shuai Chen, Nazhen Liu, Yongliang Yu. A ratiometric fluorescent probe based on carbon dots assembly for intracellular lysosomal polarity imaging with wide range response[J]. Chinese Chemical Letters, ;2022, 33(2): 778-782. doi: 10.1016/j.cclet.2021.08.076 shu

A ratiometric fluorescent probe based on carbon dots assembly for intracellular lysosomal polarity imaging with wide range response

Guangyue Zou ^{a, 1} ,
Shuai Chen ^{a, 1} ,
Nazhen Liu ^{b, *,} ,
Yongliang Yu ^{a, *,}

a.
Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Shenyang 110819, China
b.
Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China

liunazhen@qdio.ac.cn

yuyl@mail.neu.edu.cn

Received Date: 14 May 2021
Revised Date: 9 August 2021
Accepted Date: 15 August 2021
Available Online: 20 August 2021

Figures(4)

Lysosomal polarity is considered a key indicator of lysosomal function due to its significant impact on membrane fluidity and enzymatic reactions in lysosomes. Monitoring lysosomal polarity can gain insight into the related physiological and pathological processes and develop new diagnostic methods. However, current fluorescent probes with lysosomal polarity response suffer from narrow linear range, photobleaching and complicated preparation. Herein, a ratiometric fluorescent probe (r-bCDs) for intracellular lysosomal polarity imaging is designed and constructed by amide bond assembly of polarity-sensitive red fluorescent carbon dots (rCDs) and referenced blue fluorescent carbon dots (bCDs). r-bCDs show a much wider linear range of polarity response (orientation polarizability Δf from 0.020 to 0.315) than other probes, and the interference of uneven distribution and instrument factors can be effectively eliminated by ratiometric fluorescent sensing. Imaging of intracellular lysosomal polarity with r-bCDs is implemented to observe the polarity variation caused by the change of cell state and the difference between cancer cells and normal cells. This work provides a promising tool for studying the related physiological and pathological processes and developing new diagnostic methods.
- Carbon dots,
- Polarity,
- Lysosome,
- Ratiometric sensing,
- Wide linear range
1. [1]
  Z.J. Yang, J.F. Cao, Y.X. He, Chem. Soc. Rev. 43(2014) 4563-4601. doi: 10.1039/C4CS00051J
2. [2]
  Y.D. Zhuang, P.Y. Chiang, C.W. Wang, Angew. Chem. Int. Ed. 52(2013) 8124-8128. doi: 10.1002/anie.201302884
3. [3]
  L. Huang, S.W. Tam-Chang, J. Fluoresc. 21(2011) 213-222. doi: 10.1007/s10895-010-0708-z
4. [4]
  H. Zhu, J.L. Fan, H.Y. Mu, Sci. Rep. 6(2016) 35627. doi: 10.1038/srep35627
5. [5]
  M. Simons, M. Mlodzik, Annu. Rev. Genet. 42(2008) 517-540. doi: 10.1146/annurev.genet.42.110807.091432
6. [6]
  J.L. Yin, M. Peng, Y.Y. Ma, Chem. Commun. 54(2018) 12093-12096. doi: 10.1039/C8CC07398H
7. [7]
  S.J. Park, V. Juvekar, J.H. Jo, Chem. Sci. 11(2020) 596-601. doi: 10.1039/C9SC04859F
8. [8]
  C. de Duve, R. Wattiaux, Annu. Rev. Physiol. 28(1966) 435-492. doi: 10.1146/annurev.ph.28.030166.002251
9. [9]
  J.P. Luzio, P.R. Pryor, N.A. Bright, Nat. Rev. Mol. Cell Biol. 8(2007) 622-632. doi: 10.1038/nrm2217
10. [10]
  M. Li, J.L. Fan, H.D. Li, Biomaterials 164(2018) 98-105. doi: 10.1016/j.biomaterials.2018.02.044
11. [11]
  J.L. Yin, M. Peng, W.Y. Lin, Chem. Commun. 55(2019) 11063-11066. doi: 10.1039/C9CC04739E
12. [12]
  D.L. Shi, L.H. Hu, X.M. Li, Sens. Actuators B: Chem. 319(2020) 128302. doi: 10.1016/j.snb.2020.128302
13. [13]
  L. Fan, X.D. Wang, J.Y. Ge, Chem. Commun. 55(2019) 4703-4706. doi: 10.1039/C9CC00962K
14. [14]
  C. Reichardt, Chem. Rev. 94(1994) 2319-2358. doi: 10.1021/cr00032a005
15. [15]
  Y.A. Kyriukha, O.A. Kucherak, T.I. Yushchenko, Sens. Actuators B: Chem. 265(2018) 691-698. doi: 10.1016/j.snb.2018.03.058
16. [16]
  Y.Q. Sun, H.Y. Qin, X. Geng, ACS Appl. Mater. Interfaces 12(2020) 31738-31744. doi: 10.1021/acsami.0c05005
17. [17]
  Y.X. Lin, Q. Zhou, D.P. Tang, Anal. Chem. 89(2017) 5637-5645. doi: 10.1021/acs.analchem.7b00942
18. [18]
  S.Z. Lv, Y. Li, K.Y. Zhang, ACS Appl. Mater. Interfaces 9(2017) 38336-38343. doi: 10.1021/acsami.7b13272
19. [19]
  S.Y. Lu, L.Z. Sui, J.J. Liu, Adv. Mater. 29(2017) 1603443. doi: 10.1002/adma.201603443
20. [20]
  W.D. Li, Y. Liu, B.Y. Wang, Chin. Chem. Lett. 30(2019) 2323-2327. doi: 10.1016/j.cclet.2019.06.040
21. [21]
  B.Y. Wang, J. Li, Z.Y. Tang, Sci. Bull. 64(2019) 1285-1292. doi: 10.1016/j.scib.2019.07.021
22. [22]
  L. Ai, Y.S. Yang, B.Y. Wang, Sci. Bull. 66(2021) 839-856. doi: 10.1016/j.scib.2020.12.015
23. [23]
  H. Wang, C. Sun, X.R. Chen, Nanoscale 9(2017) 1909-1915. doi: 10.1039/C6NR09200D
24. [24]
  J. Xia, S. Chen, G.Y. Zou, Nanoscale 10(2018) 22484-22492. doi: 10.1039/C8NR08208A
25. [25]
  X.W. Hua, Y.W. Bao, J. Zeng, ACS Appl. Mater. Interfaces 11(2019) 32647-32658. doi: 10.1021/acsami.9b09590
26. [26]
  E S., Q.X. Mao, J.H. Wang, Nanoscale 12(2020) 6852-6860. doi: 10.1039/C9NR10982J
27. [27]
  J.H. Liu, D.Y. Li, J.H. He, ACS Appl. Mater. Interfaces 12(2020) 4815-4820. doi: 10.1021/acsami.9b18934
28. [28]
  Y.Q. Dong, H.C. Pang, H.B. Yang, Angew. Chem. Int. Ed. 52(2013) 7800-7804. doi: 10.1002/anie.201301114
29. [29]
  W. Xu, Z. Zeng, J.H. Jiang, Angew. Chem. Int. Ed. 55(2016) 13658-13699. doi: 10.1002/anie.201510721
30. [30]
  H. Hillaireau, P. Couvreur, Cell. Mol. Life Sci. 66(2009) 2873-2896. doi: 10.1007/s00018-009-0053-z
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