Citation: Wanting Hu, Dan Gao, Zhaochen Su, Rui Qian, Yu Wang, Qionglin Liang. A cellular chip-MS system for investigation of Lactobacillus rhamnosus GG and irinotecan synergistic effects on colorectal cancer[J]. Chinese Chemical Letters, ;2022, 33(4): 2096-2100. doi: 10.1016/j.cclet.2021.08.041 shu

A cellular chip-MS system for investigation of Lactobacillus rhamnosus GG and irinotecan synergistic effects on colorectal cancer

Wanting Hu ^{a, b} ,
Dan Gao ^{a, *,} ,
Zhaochen Su ^a ,
Rui Qian ^a ,
Yu Wang ^b ,
Qionglin Liang ^{b, *,}

a.
State Key Laboratory of Chemical Oncogenomics, the Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China
b.
Department of Chemistry, Center for Synthetic and Systems Biology, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, China

gao.dan@sz.tsinghua.edu.cn

liangql@tsinghua.edu.cn

Received Date: 16 May 2021
Revised Date: 20 July 2021
Accepted Date: 8 August 2021
Available Online: 12 August 2021

Figures(4)

Colorectal cancer (CRC) is still the leading cause of cancer death worldwide, but the clinical effect of drug therapy such as irinotecan is not an ideal way at present. In recent years, probiotics have attracted much attention, and the combination of probiotics may play an important role in the prevention and treatment of CRC. This work proposed a cellular chip-MS system, to study the synergistic effects of probiotic Lactobacillus rhamnosus GG (L.GG) and irinotecan on HCT116 cells by cell viability and on-line mass spectrometry (MS) analysis. The double-layer chip sandwiched with a polycarbonate membrane can co-culture HCT116 cells and L.GG. And the solid phase microextraction chip can be used for desalination and concentration. Finally, the extracted chemicals were entered the electrospray ionization quadrupole time-of-flight MS to detect irinotecan metabolites. The results showed that with the increasing concentration of co-cultured L.GG, the percentage of living HCT116 cells decreased, but the relative amount of metabolized SN-38 by HCT116 cells increased. Therefore, the microfluidic system can be used to detect and monitor the synergistic effect of irinotecan-L.GG combination on HCT116 cells. In summary, our study provided experimental evidence for the first time with potential applications of irinotecan-L.GG combination in CRC treatment, and the cellular chip-MS system as a powerful tool can be used in the experiments of probiotics as new drugs.
- Irinotecan,
- Lactobacillus rhamnosus,
- Microfluidics,
- Mass spectrometry,
- Colorectal cancer
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