Citation: Chenglong Sun, Chunxia Ma, Lili Li, Yuhao Han, Daijie Wang, Xiao Wan. A novel on-tissue cycloaddition reagent for mass spectrometry imaging of lipid C=C position isomers in biological tissues[J]. Chinese Chemical Letters, ;2022, 33(4): 2073-2076. doi: 10.1016/j.cclet.2021.08.034 shu

A novel on-tissue cycloaddition reagent for mass spectrometry imaging of lipid C=C position isomers in biological tissues

Chenglong Sun ^{a, b, *,} ,
Chunxia Ma ^{a, b} ,
Lili Li ^{a, b} ,
Yuhao Han ^b ,
Daijie Wang ^{a, b} ,
Xiao Wan ^{a, b, *,}

a.
School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Ji'nan 250014, China
b.
Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Ji'nan 250014, China

chenglongsun1989@163.com

wangx@sdas.org

Received Date: 25 March 2021
Revised Date: 21 July 2021
Accepted Date: 8 August 2021
Available Online: 12 August 2021

Figures(5)

Application of matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) to investigate the spatiotemporal alterations of lipids in biological tissues has brought many significant results. However, the presence of structural isomers varying in C=C double bond (DB) locations makes isomer-resolved MSI an urgent need. Herein, we introduce a new type of light-driven on-tissue [2 + 2] cycloaddition reaction coupled with MALDI-MS/MS imaging to identify lipid DB position isomers and their spatial signatures in biological tissues. 3-Benzoylpyridine was introduced as a novel derivatization reagent, and it exhibited great reactivity toward lipid C=C bond to form oxetanes under both ultraviolet light and visible light irradiation. With this approach, DB position isomers of lipids were imaged with highly differential levels in distinct regions of rat brain, providing an accurate and spatially resolved approach to study tissue lipidomics.
- MALDI-MSI,
- On-tissue cycloaddition,
- Lipid,
- C=C position isomers,
- Biological tissues
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