Citation: Xing Wei, Meng Yang, Ze Jiang, Jinhui Liu, Xuan Zhang, Mingli Chen, Jianhua Wang. A modular single-cell pipette microfluidic chip coupling to ETAAS and ICP-MS for single cell analysis[J]. Chinese Chemical Letters, ;2022, 33(3): 1373-1376. doi: 10.1016/j.cclet.2021.08.024 shu

A modular single-cell pipette microfluidic chip coupling to ETAAS and ICP-MS for single cell analysis

Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Shenyang 110819, China

chenml@mail.neu.edu.cn

jianhuajrz@mail.neu.edu.cn

Received Date: 28 May 2021
Revised Date: 11 July 2021
Accepted Date: 6 August 2021
Available Online: 12 August 2021

Figures(4)

Accurate single-cell capture is a crucial step for single cell biological and chemical analysis. Conventional single-cell capturing often confront operational complexity, limited efficiency, cell damage, large scale but low accuracy, incompetence in the acquirement of nano-upgraded single-cell liquid. Flow cytometry has been widely used in large-scale single-cell detection, while precise single-cell isolation relies on both a precision operating platform and a microscope, which is not only extremely inefficient, but also not conducive to couple with modern analytical instruments. Herein, we develop a modular single-cell pipette (mSCP) microfluidic chip with high efficiency and strong applicability for accurate direct capture of single viable cell from cell suspensions into nanoliter droplets (30-1000 nL). The mSCP is used as a sampling platform for the detection of CdTe quantum dots in single cells with electrothermal atomic absorption spectrometry (ETAAS) for the first time. It also ensures precise single-cell sampling and detection by inductively coupled plasma mass spectrometry (ICP-MS).
- Single-cell pipette,
- Microfluidic chip,
- Single-cell capture,
- ETAAS,
- ICP-MS,
- CdTe QDs
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沈阳化工大学材料科学与工程学院沈阳 110142