Citation: Chenghui Zhang, Pengjuan Ni, Bo Wang, Wendong Liu, Yuanyuan Jiang, Chuanxia Chen, Jian Sun, Yizhong Lu. Enhanced oxidase-like activity of g-C₃N₄ nanosheets supported Pd nanosheets for ratiometric fluorescence detection of acetylcholinesterase activity and its inhibitor[J]. Chinese Chemical Letters, ;2022, 33(2): 757-761. doi: 10.1016/j.cclet.2021.08.017 shu

Enhanced oxidase-like activity of g-C₃N₄ nanosheets supported Pd nanosheets for ratiometric fluorescence detection of acetylcholinesterase activity and its inhibitor

Chenghui Zhang ^a ,
Pengjuan Ni ^a ,
Bo Wang ^a ,
Wendong Liu ^a ,
Yuanyuan Jiang ^a ,
Chuanxia Chen ^{a, *,} ,
Jian Sun ^{b, *,} ,
Yizhong Lu ^{a, *,}

a.
School of Materials Science and Engineering, University of Jinan, Ji'nan 250022, China
b.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

mse_chencx@ujn.edu.cn

jiansun@ciac.ac.cn

mse_luyz@ujn.edu.cn

Received Date: 12 May 2021
Revised Date: 20 July 2021
Accepted Date: 5 August 2021
Available Online: 11 August 2021

Figures(4)

The undesirable enzymatic activity of nanozymes under near neutral pH condition and the traditional single signal output always restrict the analytical application of nanozyme-based biosensors. Herein, graphitic carbon nitride nanosheets supported palladium nanosheets composite (Pd/g-C₃N₄) with both oxidase-like activity and fluorescent property is synthesized. Notably, Pd/g-C₃N₄ exhibits enhanced oxidase-like activity compared to Pd NSs under pH 7.4. By combining Pd/g-C₃N₄ with o-phenylenediamine (OPD), a ratiometric fluorescence assay for acetylcholinesterase (AChE) activity detection is developed. Pd/g-C₃N₄ can catalyze oxidation of nonfluorescent OPD to fluorescent oxidized OPD (oxOPD, Em = 565 nm), which can quench fluorescence of g-C₃N₄ supporter (Em = 441 nm) through fluorescence resonance energy transfer (FRET). However, in presence of AChE, acetylthiocholine can be hydrolyzed into thiocholine, which will block the oxidase-like activity of Pd/g-C₃N₄ and then hamper the FRET process. This ratiometric fluorescence assay is also viable to screen AChE inhibitor. This work will guide design of ratiometric fluorescence assay based on nanozymes with improved enzymatic activity.
- Oxidase-like activity,
- Ratiometric fluorescence,
- Acetylcholinesterase,
- g-C₃N₄ nanosheets,
- Pd nanosheets
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沈阳化工大学材料科学与工程学院沈阳 110142

Enhanced oxidase-like activity of g-C3N4 nanosheets supported Pd nanosheets for ratiometric fluorescence detection of acetylcholinesterase activity and its inhibitor

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通讯作者: 陈斌, bchen63@163.com

Enhanced oxidase-like activity of g-C₃N₄ nanosheets supported Pd nanosheets for ratiometric fluorescence detection of acetylcholinesterase activity and its inhibitor