Citation: Wenying Mi, Shuang Tang, Shaoshi Guo, Hejing Li, Na Shao. In situ synthesis of red fluorescent gold nanoclusters with enzyme-like activity for oxidative stress amplification in chemodynamic therapy[J]. Chinese Chemical Letters, ;2022, 33(3): 1331-1336. doi: 10.1016/j.cclet.2021.07.073 shu

In situ synthesis of red fluorescent gold nanoclusters with enzyme-like activity for oxidative stress amplification in chemodynamic therapy

College of Chemistry, Beijing Normal University, Beijing 100875, China

shaona@bnu.edu.cn

Received Date: 31 May 2021
Revised Date: 15 July 2021
Accepted Date: 31 July 2021
Available Online: 8 August 2021

Figures(8)

Chemodynamic therapy (CDT) has attracted tremendous interest in cancer therapy because it is independent of oxygen and photoirradiation. However, the therapeutic efficacy of CDT is restricted by insufficient H₂O₂ levels in tumor cells. Herein, employing endogenous GSH as a template and cationic polymeric chitosan (CS) as crosslinker and stabilizer exhibiting easy cell uptake, red luminescent gold nanoclusters (denoted CS-GSH@AuNCs) were successfully synthesized in HeLa cells. The in situ synthesized CS-GSH@AuNCs exhibited both superoxidase dismutase (SOD) and peroxidase (POD)-like activity, which could promote the production of H₂O₂ from superoxide anion radicals (O₂^·−) and then ^·OH. The combination of GSH elimination and H₂O₂ elevation boosted the generation of ^·OH, which could trigger cancer cell apoptosis and death. The enzyme-like activity of CS-GSH@AuNCs could be effectively activated under acidic conditions, and showed a high killing effect on tumor cells but minimal toxicity to normal cells. The developed GSH consumption and ^·OH promotion theranostic platform is an innovative route for enhanced CDT by the amplification of oxidative stress.
- Chemodynamic therapy,
- In situ synthesis,
- Gold nanoclusters,
- Superoxidase dismutase and peroxidase (POD)-like activity,
- Oxidative stress amplification
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