Citation: Tianyou Chen, Chunxia Huang, Yanran Wang, Jing Wu. Microfluidic methods for cell separation and subsequent analysis[J]. Chinese Chemical Letters, ;2022, 33(3): 1180-1192. doi: 10.1016/j.cclet.2021.07.067 shu

Microfluidic methods for cell separation and subsequent analysis

School of Science, China University of Geosciences (Beijing), Beijing 100083, China

wujing@cugb.edu.cn

Received Date: 24 May 2021
Revised Date: 20 June 2021
Accepted Date: 30 July 2021
Available Online: 5 August 2021

Figures(11)

Cell is the most basic unit of the morphological structure and life activity of an organism. Learning the composition, structure and function of cells, exploring the life activities of cells and studying the interaction between cells are of great significance for human cognition and control of the life activities of organisms. Therefore, rapid, convenient, inexpensive, high-precision and reliable methods of cell separation and analysis are being developed to obtain accurate information for the study of cytology and pathology. Microfluidic chip is a new emerging technology in recent years. It has a micromanufacturing structure, which can not only realize the precise space-time control of fluid and cells, but also reproduces the three-dimensional dynamic microenvironment of cell growth in the body. In addition, the microfluidic chip has unique microphysical properties and facilitates the integration of microdevices, which provides the possibility of real-time monitoring, continuous culture, separation and enrichment, and even in situ analysis of cells. In this review, we summarized recent advances in the development of different techniques for cell isolation and analysis on microfluidic platforms. Focus was put on biochemical and physical methods for cell separation on microfluidic chips. Subsequent cell analysis depending on fluorescence, Raman, cytomicroscopic imaging, mass spectrometry and electrochemical methods also was remarked. Through analyzing and learning the advantages and disadvantages of different technologies, we hope that microfluidic chips will continue to be improved and expanded for medical and clinical applications.
- Microfluidic,
- Cell separation,
- Biochemical separation,
- Physical separation,
- Cell analysis
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