Citation: Yuzhi Xu, Yanfei Zhang, Huihui Yang, Wen Yin, Leli Zeng, Shuo Fang, Si-Yang Liu, Zong Dai, Xiaoyong Zou, Yihang Pan. Two-dimensional coordination polymer-based nanosensor for sensitive and reliable nucleic acids detection in living cells[J]. Chinese Chemical Letters, ;2022, 33(2): 968-972. doi: 10.1016/j.cclet.2021.07.041 shu

Two-dimensional coordination polymer-based nanosensor for sensitive and reliable nucleic acids detection in living cells

Yuzhi Xu ^a ,
Yanfei Zhang ^c ,
Huihui Yang ^c ,
Wen Yin ^c ,
Leli Zeng ^a ,
Shuo Fang ^d ,
Si-Yang Liu ^{b, *,} ,
Zong Dai ^{b, *,} ,
Xiaoyong Zou ^c ,
Yihang Pan ^{a, *,}

a.
Precision Medicine Center, Scientific Research Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, China
b.
Key Laboratory of Sensing Technology and Biomedical Instrument of Guangdong Province, School of Biomedical Engineering, Sun Yat-sen University, Guangzhou 510006, China
c.
School of Chemistry, Sun Yat-sen University, Guangzhou 510275, China
d.
Department of Oncology, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, China

liusiyang@mail.sysu.edu.cn

daizong@mail.sysu.edu.cn

panyih@mail.sysu.edu.cn

Received Date: 14 April 2021
Revised Date: 6 June 2021
Accepted Date: 15 July 2021
Available Online: 22 July 2021

Figures(4)

A reliable and sensitive strategy which can assess nucleic acid levels in living cells would be essential for fundamental research of biomedical applications. Some nanomaterial-based fluorescence biosensors recently developed for detecting nucleic acids, however, are often with expensive, complicated and time-consuming preparation process. Here, by using a facile bottom-up synthesis method, a two-dimensional (2D) coordination polymer (CP) nanosheet, [Cu(tz)] (Htz = 1, 2, 4-triazole), was successfully prepared after optimizing reaction conditions. These ultrathin CP nanosheets with thickness of 4.7 ± 1.1 nm could readily form nanosensors by assembly with DNA probes, which exhibited a low limit of detection (LOD) for p53 DNA fragment as 144 pmol/L. Furthermore, by integrating [Cu(tz)] nanosheets with hybridization chain reaction (HCR) probes, miR-21, one kind of microRNA upregulated in many cancer cells, can be sensitively detected with a LOD of 100 pmol/L and monitored in living cells, giving consistent results with those obtained by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analysis. Thus [Cu(tz)] nanosheets, which not only possess much better nucleic acids sensing performance than bulk cystals, but also exhibit nucleic acid delivery functions, could be used as a novel nanoplatform in biomedical imaging and sensing applications.
- Coordination polymers,
- Nanosheets,
- Fluorescence biosensor,
- Nucleic acids detection,
- Living cells
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