Citation: Ling Feng, Ping Wang, Yi Feng, Jie Zhang, Qingxin Chen, Yusheng Xie, Jingdong Luo, Jiang Xia, Shao Q. Yao, Hongyan Sun. A reversible microarray immobilization strategy based on thiol-quinone reaction[J]. Chinese Chemical Letters, ;2022, 33(1): 213-216. doi: 10.1016/j.cclet.2021.06.056 shu

A reversible microarray immobilization strategy based on thiol-quinone reaction

Ling Feng ^{a, b, c} ,
Ping Wang ^{b, c} ,
Yi Feng ^d ,
Jie Zhang ^{b, c} ,
Qingxin Chen ^{b, c} ,
Yusheng Xie ^{b, c} ,
Jingdong Luo ^{b, c} ,
Jiang Xia ^e ,
Shao Q. Yao ^f ,
Hongyan Sun ^{b, c, *,}

a.
Cancer and Aging Research Institution, School of Life Science, Shandong University of Technology, Zibo 255000, China
b.
Department of Chemistry and COSDAF (Centre of Super-Diamond and Advanced Films), City University of Hong Kong, Hong Kong, China
c.
Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institute of City University of Hong Kong, Shenzhen 518057, China
d.
College of Food Engineering, Qingdao Institute of Technology, Qingdao 266300, China
e.
Department of Chemistry, The Chinese University of Hong Kong, Hong Kong, China
f.
Department of Chemistry, National University of Singapore, Singapore 117543, Singapore

hongysun@cityu.edu.hk

Received Date: 11 April 2021
Revised Date: 8 June 2021
Accepted Date: 21 June 2021
Available Online: 29 June 2021

Figures(5)

Microarray technology has been widely applied in biomedical research. The key to microarray study is to develop efficient immobilization method. In this study, we designed a new reversible microarray immobilization method based on thiol-quinone reaction. A quinone-functionalized slide was fabricated through H₂O₂ treatment of dopamine-coated slides. Various thiol-containing molecules can be anchored onto the quinone-functionalized slides via thioether linker, which could be cleaved under H₂O₂ treatment to regenerate quinone groups on the surface. The highly versatile approach can be widely used for immobilization of various thiol-containing molecules.
- Reversible Microarray,
- Biochip,
- Thiol-quinone reaction,
- Peptides,
- Thiol-proteins
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