Citation: Min Xie, Chaobo Huang, Yongqi Liang, Shuang Li, Liping Sheng, Yi Cao. MoS₂ nanosheets and bulk materials altered lipid profiles in 3D Caco-2 spheroids[J]. Chinese Chemical Letters, ;2022, 33(1): 293-297. doi: 10.1016/j.cclet.2021.06.049 shu

MoS₂ nanosheets and bulk materials altered lipid profiles in 3D Caco-2 spheroids

Min Xie ^{a, b, c} ,
Chaobo Huang ^b ,
Yongqi Liang ^c ,
Shuang Li ^c ,
Liping Sheng ^{a, *,} ,
Yi Cao ^{d, *,}

a.
National Local Joint Engineering Laboratory for New Petro-chemical Materials and Fine Utilization of Resources, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China
b.
College of Chemical Engineering, Nanjing Forestry University (NFU), Nanjing 210037, China
c.
Key Laboratory of Environment-Friendly Chemistry and Applications of Ministry Education, Laboratory of Biochemistry, College of Chemistry, Xiangtan University, Xiangtan 411105, China
d.
Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, University of South China, Hengyang 421001, China

sleeping1217@126.com

caoyi39@126.com

Received Date: 20 April 2021
Revised Date: 16 May 2021
Accepted Date: 18 June 2021
Available Online: 26 June 2021

Figures(4)

MoS₂ nanosheets (NSs) are novel 2D nanomaterials (NMs) with potential uses in many areas, and therefore oral exposure route to MoS₂ NSs is plausible. Currently, MoS₂ NSs are considered as biocompatible NMs, but there is lacking of systemic investigations to study the interactions of MoS₂ NSs with intestinal cells. In this study, we exposed the 3D Caco-2 spheroids to MoS₂ NSs or MoS₂ powders (denoted as MoS₂-bulk), and investigated the potential adverse effects of MoS₂-materials based on transcriptomics and lipidomics analysis. As expected, both MoS₂ NSs and MoS₂-bulk were dose-dependently internalized into 3D Caco-2 spheroids but did not induce cytotoxicity, membrane disruption or decrease of thiols. However, the Gene Ontology (GO) and Kyoto Encyclopedia of Gene and Genomes (KEGG) analysis indicated that nutrient absorption and metabolism was decreased. One of the most significantly decreased KEGG pathways is fat digestion and absorption (map04975), and Western blotting analysis further showed that fatty acid binding protein 1 and apolipoprotein A1, key proteins involved in fat digestion and absorption, were down-regulated by MoS₂ NSs or MoS₂-bulk. In addition, BODIPY 493/503 staining suggested that exposure to MoS₂ NSs and MoS₂-bulk decreased lipid levels in the spheroids. However, lipidomics data indicated that MoS₂ materials only decreased 8 lipid classes, including lysophosphatidylcholine, lysodimethylphosphatidylethanolamine, N-acylethanolamine, ceramide phosphoethanolamines, gangliosides, lysosphingomyelin and sulfatide, whereas most of the lipid classes were indeed increased. In addition, MoS₂ NSs was more potent to decrease the lipid classes compared with MoS₂-bulk. Combined, the results from this study showed that MoS₂ NSs and bulk materials were non-cytotoxic but altered lipid profiles in 3D Caco-2 spheroids.
- MoS₂ nanosheets (NSs),
- 3D Caco-2 spheroids,
- Transcriptomics,
- Lipidomics,
- Fat digestion and absorption (map04975)
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MoS2 nanosheets and bulk materials altered lipid profiles in 3D Caco-2 spheroids

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MoS₂ nanosheets and bulk materials altered lipid profiles in 3D Caco-2 spheroids