Citation: Xingxing Zhang, Tianbing Ren, Feiyu Yang, Lin Yuan. Rational design of far red to near-infrared rhodamine analogues with huge Stokes shifts for single-laser excitation multicolor imaging[J]. Chinese Chemical Letters, ;2021, 32(12): 3890-3894. doi: 10.1016/j.cclet.2021.06.038 shu

Rational design of far red to near-infrared rhodamine analogues with huge Stokes shifts for single-laser excitation multicolor imaging

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China

rentianbing@hnu.edu.cn

lyuan@hnu.edu.cn

Received Date: 21 March 2021
Revised Date: 1 June 2021
Accepted Date: 14 June 2021
Available Online: 23 June 2021

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Rhodamine dyes have been widely employed in biological imaging and sensing. However, it is always a challenge to design rhodamine derivatives with huge Stokes shift to address the draconian requirements of single-excitation multicolor imaging. In this work, we described a generally strategy to enhance the Stokes shift of rhodamine dyes by completely breaking their electronic symmetry. As a result, the Stokes shift of novel rhodamine dye DQF-RB-Cl is up to 205 nm in PBS, which is the largest in all the reported rhodamine derivatives. In addition, we successfully realized the single excitation trichromatic imaging of mitochondria, lysosomes and cell membranes by combining DQF-RB-Cl with commercial lysosomal targeting probe Lyso-Tracker Green and membrane targeting dye Dil. This is the organic synthetic dyes for SLE-trichromatic imaging in cells for the first time. These results demonstrate the potential of our design as a useful strategy to develop huge Stokes shift fluorophore for bioimaging.
- Fluorescent dyes,
- Rhodamine analogues,
- Huge stokes shifts,
- Single-laser excitation,
- Multicolor imaging
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