Citation: Siyu Luo, Meng Sha, Fei Tian, Xialian Li, Lijie Fu, Yingqiu Gu, Lu-Lu Qu, Guo-Hai Yang, Chengzhou Zhu. Nitrogen and boron co-doped graphene nanoribbons as peroxidase-mimicking nanozymes for enhanced biosensing[J]. Chinese Chemical Letters, ;2022, 33(1): 344-348. doi: 10.1016/j.cclet.2021.06.018 shu

Nitrogen and boron co-doped graphene nanoribbons as peroxidase-mimicking nanozymes for enhanced biosensing

Siyu Luo ^a ,
Meng Sha ^b ,
Fei Tian ^a ,
Xialian Li ^a ,
Lijie Fu ^a ,
Yingqiu Gu ^a ,
Lu-Lu Qu ^{a, *,} ,
Guo-Hai Yang ^{a, *,} ,
Chengzhou Zhu ^{b, *,}

a.
School of Chemistry & Materials Science, Jiangsu Normal University, Xuzhou 221116, China
b.
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, International Joint Research Center for Intelligent Biosensing Technology and Health, College of Chemistry, Central China Normal University, Wuhan 430079, China

luluqu@jsnu.edu.cn

yangguohai@jsnu.edu.cn

czzhu@mail.ccnu.edu.cn

Received Date: 3 March 2021
Revised Date: 8 April 2021
Accepted Date: 4 June 2021
Available Online: 17 June 2021

Figures(4)

The rational design of nanozymes with superior activities is essential for improving bioassay performances. Herein, nitrogen and boron co-doped graphene nanoribbons (NB-GNRs) are prepared by a hydrothermal method using urea as the nitrogen source and boric acid as the boron source, respectively. The introduction of co-doped and edge structures provides high defects and active sites. The resultant NB-GNRs nanozymes show superior peroxidase-like activities to nitrogen-doped and boron-doped counterparts due to the synergistic effects. By taking advantage of their peroxidase-like activities, NB-GNRs are used for the first time to develop enzyme-linked immunosorbent assay for the detection of interleukin-6. The biosensors exhibit a high performance with a linear range from 0.001 ng/mL to 1000 ng/mL and a detection limit of 0.3 pg/mL. Due to their low cost and high stability, the proposed nanomaterials show great promise in biocatalysis, immunoassay development and environmental monitoring.
- Graphene nanoribbons,
- Heteroatom doping,
- Nanozymes,
- Biosensing,
- Interleukin-6
1. [1]
  L. Gao, J. Zhuang, L. Nie, et al., Nat. Nanotechnol.2 (2007) 577–583. doi: 10.1038/nnano.2007.260
2. [2]
  J. Wu, X. Wang, Q. Wang, et al., Chem. Soc. Rev. 48 (2019) 1004–1076. doi: 10.1039/c8cs00457a
3. [3]
  Q. Chen, X. Zhang, S. Li, et al., Chem. Eng. J. 395 (2020) 125130. doi: 10.1016/j.cej.2020.125130
4. [4]
  R. Andre, F. Natalio, M. Humanes, et al., Adv. Funct. Mater. 21 (2011) 501–509. doi: 10.1002/adfm.201001302
5. [5]
  N. Singh, M.A. Savanur, S. Srivastava, P. D'Silva, G. Mugesh, Angew. Chem. Int. Ed. 56 (2017) 14267–14271. doi: 10.1002/anie.201708573
6. [6]
  X. Liang, L. Han, Adv. Funct. Mater. 30 (2020) 2001933. doi: 10.1002/adfm.202001933
7. [7]
  J. Li, Y. Cao, S.S. Hinman, et al., Biosens. Bioelectron. 100 (2018) 304–311. doi: 10.1051/metal/2017096
8. [8]
  X. Xu, L. Wang, X. Zou, et al., Sens. Actuators B Chem. 298 (2019) 126876. doi: 10.1016/j.snb.2019.126876
9. [9]
  O. Adeniyi, S. Sicwetsha, P. Mashazi, ACS Appl. Mater. Interfaces12 (2020) 1973–1987. doi: 10.1021/acsami.9b15123
10. [10]
  L. Jiao, L. Zhang, W. Du, et al., Nanoscale11 (2019) 8798–8802. doi: 10.1039/c8nr08741e
11. [11]
  W. Zhang, S. Hu, J.J. Yin, et al., J. Am. Chem. Soc. 138 (2016) 5860–5865. doi: 10.1021/jacs.5b12070
12. [12]
  P. Zhang, D. Sun, A. Cho, et al., Nat. Commun. 10 (2019) 940. doi: 10.1038/s41467-019-08731-y
13. [13]
  B. Tan, H. Zhao, W. Wu, et al., Nanoscale9 (2017) 18699–18710. doi: 10.1039/C7NR05541B
14. [14]
  Y. Zhong, X. Tang, J. Li, et al., Chem. Commun. 54 (2018) 13813–13816. doi: 10.1039/c8cc07779g
15. [15]
  Y. Zhong, J. Li, A. Lambert, Z. Yang, Q. Cheng, J. Mater. Chem. B7 (2019) 7257–7266. doi: 10.1039/c9tb01029g
16. [16]
  J. Mu, Y. Wang, M. Zhao, L. Zhang, Chem. Commun. 48 (2012) 2540–2542. doi: 10.1039/c2cc17013b
17. [17]
  L. Han, H. Zhang, D. Chen, F. Li, Adv. Funct. Mater. 28 (2018) 1800018. doi: 10.1002/adfm.201800018
18. [18]
  M.I. Kim, Y. Ye, M.A. Woo, J. Lee, H.G. Park, Adv. Healthc. Mater. 3 (2014) 36–41. doi: 10.1002/adhm.201300100
19. [19]
  W. Shi, Q. Wang, Y. Long, et al., Chem. Commun. 47 (2011) 6695–6697. doi: 10.1039/c1cc11943e
20. [20]
  Y. Song, K. Qu, C. Zhao, J. Ren, X. Qu, Adv. Mater. 22 (2010) 2206. doi: 10.1002/adma.200903783
21. [21]
  M.S. Kim, S. Cho, S.H. Joo, et al., ACS Nano13 (2019) 4312–4321. doi: 10.1021/acsnano.8b09519
22. [22]
  F. Qu, T. Li, M. Yang, Biosens. Bioelectron. 26 (2011) 3927–3931. doi: 10.1016/j.bios.2011.03.013
23. [23]
  Y. Hu, X.J. Gao, Y. Zhu, et al., Chem. Mater. 30 (2018) 6431–6439. doi: 10.1021/acs.chemmater.8b02726
24. [24]
  H. Wang, P. Li, D. Yu, et al., Nano Lett. 18 (2018) 3344–3351. doi: 10.1021/acs.nanolett.7b05095
25. [25]
  L. Chen, Y. Hernandez, X. Feng, K. Muellen, Angew. Chem. Int. Ed. 51 (2012) 7640–7654. doi: 10.1002/anie.201201084
26. [26]
  S. Rostami, A. Mehdinia, A. Jabbari, Mater. Sci. Eng. C Mater. 114 (2020) 111034. doi: 10.1016/j.msec.2020.111034
27. [27]
  Z. Pan, N. Liu, L. Fu, Z. Liu, J. Am. Chem. Soc. 133 (2011) 17578–17581. doi: 10.1021/ja207517u
28. [28]
  D.B. Shinde, J. Debgupta, A. Kushwaha, M. Aslam, V.K. Pillai, J. Am. Chem. Soc. 133 (2011) 4168–4171. doi: 10.1021/ja1101739
29. [29]
  Z. Xiang, Q. Dai, J.F. Chen, L. Dai, Adv. Mater. 28 (2016) 6253. doi: 10.1002/adma.201505788
30. [30]
  K. Gao, B. Wang, L. Tao, et al., Adv. Mater. 31 (2019) 1805121. doi: 10.1002/adma.201805121
31. [31]
  L. Ren, X. Yi, L. Tong, et al., Appl. Catal. B279 (2020) 119352. doi: 10.1016/j.apcatb.2020.119352
32. [32]
  M. Masteri-Farahani, F. Ghorbani, N. Mosleh, Spectrochim. Acta Part A245 (2020) 118892.
33. [33]
  J.H. Yu, C.Z. He, C.Y. Pu, et al., Chin. Chem. Lett. 32 (2021) 3149–3154. doi: 10.1016/j.cclet.2021.02.046
34. [34]
  H.Y. Yang, C.Z. He, L. Fu, et al., Chin. Chem. Lett. 32 (2021) 3202–3206. doi: 10.1016/j.cclet.2021.03.038
35. [35]
  R. Wang, C.Z. He, W.X. Chen, C.X. Zhao, J.R. Huo. Chin. Chem. Lett.(2021) doi:10.1016/j.cclet.2021.05.024. doi: 10.1016/j.cclet.2021.05.024
36. [36]
  H. Yan, L. Wang, Y. Chen, et al., Research2020, 1–11.
37. [37]
  Y. Chen, L. Jiao, H. Yan, et al., Anal. Chem. 92 (2020) 13518–13524. doi: 10.1021/acs.analchem.0c02982
38. [38]
  D.V. Kosynkin, A.L. Higginbotham, A. Sinitskii, et al., Nature458 (2009) 872–875. doi: 10.1038/nature07872
39. [39]
  Z. Zhang, Y. Li, S. Zhu, et al., Chin. Chem. Lett. 30 (2019) 505–508. doi: 10.1016/j.cclet.2018.06.008
40. [40]
  R. Bi, R. Zhang, J. Shen, et al., J. Membr. Sci. 572 (2019) 504–511. doi: 10.1016/j.memsci.2018.11.044
41. [41]
  A. Yazdi, K. Chizari, A. Jalilov, J. Tour, U. Sundararaj, Anal. Chem. 92 (2020), 5319–5328. doi: 10.1021/acs.analchem.9b05858
42. [42]
  D. Zhang, Q. Ma, H. Fan, H. Yang, S. Liu, Carbon71 (2014) 120–126. doi: 10.1016/j.carbon.2014.01.019
43. [43]
  R. Zhang, C.L. Sun, Y.J. Lu, W. Chen, Anal. Chem. 87 (2015) 12262–12269. doi: 10.1021/acs.analchem.5b03390
44. [44]
  Y. Zheng, Y. Jiao, L. Ge, M. Jaroniec, S.Z. Qiao, Angew. Chem. Int. Ed. 52 (2013) 3110–3116. doi: 10.1002/anie.201209548
45. [45]
  C.H. Choi, M.W. Chung, H.C. Kwon, S.H. Park, S.I. Woo, J. Mater. Chem. A1 (2013) 3694–3699. doi: 10.1039/c3ta01648j
46. [46]
  H. Tabassum, W. Guo, W. Meng, et al., Adv. Energy Mater. 7 (2017) 1601671. doi: 10.1002/aenm.201601671
47. [47]
  Y. Liu, M. Zhou, W. Cao, et al., Anal. Chem. 91 (2019) 8170–8175. doi: 10.1021/acs.analchem.9b00512
48. [48]
  C. Wang, Y. Shi, Y.Y. Dan, et al. Chem. Eur. J. 23 (2017) 6717–6723. doi: 10.1002/chem.201605380
49. [49]
  W. Hu, M.R. Younis, Y. Zhou, C. Wang, X.H. Xia, Small16 (2020) 2000553. doi: 10.1002/smll.202000553
50. [50]
  A. Byeon, J.W. Lee, J. Phys. Chem. C117 (2013) 24167–24173. doi: 10.1021/jp407425y
51. [51]
  R. Zhang, C. Zhang, F. Zheng, et al., Carbon126 (2018) 328–337. doi: 10.1007/978-981-10-8890-2_24
52. [52]
  Y. Gong, H. Fei, X. Zou, et al., Chem. Mater. 27 (2015) 1181–1186. doi: 10.1021/cm5037502
53. [53]
  D.J. George, S. Halabi, T.F. Shepard, et al., Clin. Cancer Res. 11 (2005) 1815–1820. doi: 10.1158/1078-0432.CCR-04-1560
54. [54]
  R. Yu, W. Ma, X. Liu, et al., Theranostics6 (2016) 1732–1739. doi: 10.7150/thno.16129
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