Citation: Jing Shi, Jia-Qi Ma, Yong-Chao Wang, Zi-Fei Xu, Bo Zhang, Rui-Hua Jiao, Ren-Xiang Tan, Hui-Ming Ge. Discovery of daspyromycins A and B, 2-aminovinyl-cysteine containing lanthipeptides, through a genomics-based approach[J]. Chinese Chemical Letters, ;2022, 33(1): 511-515. doi: 10.1016/j.cclet.2021.06.010 shu

Discovery of daspyromycins A and B, 2-aminovinyl-cysteine containing lanthipeptides, through a genomics-based approach

Jing Shi ^a ,
Jia-Qi Ma ^a ,
Yong-Chao Wang ^a ,
Zi-Fei Xu ^a ,
Bo Zhang ^a ,
Rui-Hua Jiao ^a ,
Ren-Xiang Tan ^a ,
Hui-Ming Ge ^{a, b, *,}

a.
State Key Laboratory of Pharmaceutical Biotechnology, Institute of Functional Biomolecules, School of Life Sciences, Nanjing University, Nanjing 210023, China
b.
Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing 210023, China

hmge@nju.edu.cn

Received Date: 6 May 2021
Revised Date: 26 May 2021
Accepted Date: 5 June 2021
Available Online: 11 June 2021

Figures(4)

Lanthipeptides are one of the largest groups of ribosomally synthesized and post-translationally modified peptides (RiPPs) and are characterized by the presence of lanthionine (Lan) or methyllanthionine residues (MeLan). Only very few lanthipeptides contain a C-terminal 2-aminovinyl-cysteine (AviCys) motif, but all of them show potent antibacterial activities. Recent advances of genome sequencing led to the rapid accumulation of new biosynthetic gene clusters (BGCs) for lanthipeptides. In this study, through our genome mining strategy, we found the AviCys containing lanthipeptides are widespread in the bacterial kingdom. A lanthipeptide-type biosynthetic gene cluster was identified from public bacterial genome database. Two new lanthipeptides, daspyromycins A and B (1 and 2) containing AviCys motif, along with two degraded products, daspyromycins C and D (3 and 4), were obtained after heterologous expression of the gene cluster in Streptomyces albus J1074. Daspyromycins A and B showed potent antimicrobial activity against a spectrum of Gram-positive and -negative bacteria including methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococci (VRE).
- Lanthipeptides,
- Genomics-based approach,
- Daspyromycin,
- RiPPs,
- 2-Aminovinyl-cysteine motif
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