Citation: Juanzu Liu, Hongmin Meng, Lin Zhang, Shasha Li, Juan Chen, Yi Zhang, Jianjun Li, Lingbo Qu, Zhaohui Li. Dual-readout test strips platform for portable and highly sensitive detection of alkaline phosphatase in human serum samples[J]. Chinese Chemical Letters, ;2021, 32(11): 3421-3425. doi: 10.1016/j.cclet.2021.05.019 shu

Dual-readout test strips platform for portable and highly sensitive detection of alkaline phosphatase in human serum samples

Institute of Chemical Biology and Clinical Application at the First Affiliated Hospital, Zhengzhou Key Laboratory of Functional Nanomaterial and Medical Theranostic, College of Chemistry, Zhengzhou University, Zhengzhou 450001, China

zhanglin@zzu.edu.cn

zhangyi@zzu.edu.cn

zhaohui.li@zzu.edu.cn

Received Date: 9 February 2021
Revised Date: 12 March 2021
Accepted Date: 12 May 2021
Available Online: 21 May 2021

Figures(5)

In this work, a very simple dual-readout lateral flow test strip (LFTS) platform was developed for sensitive detection of alkaline phosphatase (ALP) based on a portable device. In this assay, quantum dots (QDs) conjugated with bovine serum albumin (QDs-BSA) were chosen as fluorescence signal labels. In the absence of ALP, MnO₂ nanosheets aggregate on the test line and exhibit an obvious brown color, which can be observed by naked eyes to realize semi-qualitative analysis. Meanwhile, fluorescence intensity of QDs-BSA can also be effectively quenched by MnO₂ nanosheets due to inner-filter effect. Correspondingly, in the presence of ALP, ALP can catalyze the hydrolysis of ascorbic acid 2-phosphate (AAP) to generate L-ascorbic acid (AA), which can reduce MnO₂ into Mn²⁺, accompanying with the obvious fluorescence recovery of the QDs. By simply monitoring the change of colorimetric and fluorescent signal on the test line, trace amount of ALP can be quantitatively detected. Under the optimal conditions, measurable evaluation of ALP was reached in a linear range from 1 U/L to 20 U/L with a detection limit of 0.7 U/L based on fluorescence signal. Furthermore, this colorimetric/fluorescent dual-readout assay was successfully applied to monitor ALP in human serum samples, showing its great potential as a point of care biosensor for clinical diagnosis.
- Lateral flow test strip,
- MnO₂ nanosheets,
- Quantum dots,
- Dual-readout,
- Alkaline phosphatase
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