Citation: Han Lin, Haofei Hong, Lipeng Feng, Jie Shi, Zhifang Zhou, Zhimeng Wu. Synthesis of DNP-modified GM3-based anticancer vaccine and evaluation of its immunological activities for cancer immunotherapy[J]. Chinese Chemical Letters, ;2021, 32(12): 4041-4044. doi: 10.1016/j.cclet.2021.04.034 shu

Synthesis of DNP-modified GM3-based anticancer vaccine and evaluation of its immunological activities for cancer immunotherapy

Key Laboratory of Carbohydrate Chemistry & Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China

zhifangzhou@jiangnan.edu.cn

zwu@jiangnan.edu.cn

Received Date: 4 February 2021
Revised Date: 1 April 2021
Accepted Date: 18 April 2021
Available Online: 24 April 2021

Figures(5)

Tumor-associated carbohydrate antigens (TACAs) are attractive targets for vaccine development. In this context, we described a strategy combining artificial TACA and glycoengineering for cancer vaccine development. A 2, 4-ditrophenyl (DNP)-modified GM3 intermediate was synthesized chemoenzymatically and conjugated to keyhole limpet hemocyanin (KLH), and the resulting bioconjugate was tested for its potential as a vaccine candidate. Mice immunological studies revealed that the DNP-modified GM3 (GM3-NHDNP) analog elicited strong and rapid immune responses by recruiting anti-DNP antibodies to facilitate the targeted delivery of the vaccine construct to antigen processing cells (APCs). Moreover, the endogenously produced anti-DNP antibodies, together with the elicited antibodies against GM3-NHDNP, may synergistically promote tumor binding and cancer cell death when the cancer cell surfaces are glycoengineered to express the GM3-NHDNP antigen.
- GM3,
- Cancer vaccine,
- Anti-DNP antibodies,
- Glycoengineering,
- Tumor-associated carbohydrate antigen
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