Citation: Qiuyun Xiao, Xuan Zhao, Hai Xiong. Biocompatible and noncytotoxic nucleoside-based AIEgens sensor for lighting-up nucleic acids[J]. Chinese Chemical Letters, ;2021, 32(5): 1687-1690. doi: 10.1016/j.cclet.2021.02.038 shu

Biocompatible and noncytotoxic nucleoside-based AIEgens sensor for lighting-up nucleic acids

Qiuyun Xiao ^{a, b} ,
Xuan Zhao ^a ,
Hai Xiong ^{a, *,}

a.
Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China
b.
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China

hai.xiong@szu.edu.cn

Received Date: 24 December 2020
Revised Date: 15 February 2021
Accepted Date: 18 February 2021
Available Online: 22 February 2021

Figures(6)

Aggregation-induced emission luminogens (AIEgens) have been used in biomacromolecules detection. Herein, TPE-dC and TPE-dU acted as the nucleoside-based AIEgens sensors in the first case, which can be used to detect ctDNA and rRNA in vitro and light up the nucleus in vivo depending on the intermolecular binding affinity. This AIE process enables the quantitative analysis or visualization of nucleic acids in solution or gels state, respectively. Furthermore, confocal laser scanning microscopy (CLSM) images of L929 cells stained with TPE-dC or TPE-dU clearly shows that nucleoside-based AIEgens bio-probes can pass the cell membranes to reach the cell nucleus, without cytotoxicity at the imaging condition (incubation time > 12 h, and 10 μmol/L of concentration). Since the nucleus is rich in DNA/RNA, fluorescence turn-on mode has a great potential in nucleus imaging and clinical diagnosis.
- AIEgens,
- Nucleoside,
- Biosensor,
- Nucleus localization,
- Cell imaging
1. [1]
  K.G. Chernov, T.A. Redchuk, E.S. Omelina, V.V. Verkhushaa, Chem. Rev. 117(2017) 6423-6446. doi: 10.1021/acs.chemrev.6b00700
2. [2]
  X. Wu, W. Shi, X. Li, H. Ma, Acc. Chem. Res. 52(2019) 1892-1904. doi: 10.1021/acs.accounts.9b00214
3. [3]
  P. Yang, S. Zhang, X.F. Chen, X.H. Liu, Z. Wang, Y.W. Li, Mater. Horiz. 7(2020) 746-761. doi: 10.1039/C9MH01197H
4. [4]
  Y.V. Suseela, N. Narayanaswamy, S. Pratihar, T. Govindaraju, Chem. Soc. Rev. 47(2018) 1098-1131. doi: 10.1039/C7CS00774D
5. [5]
  H.W. Liu, L. Chen, C. Xu, et al., Chem. Soc. Rev. 47(2018) 7140-7180. doi: 10.1039/C7CS00862G
6. [6]
  L. Yu, P. He, Y.C. Xu, et al., Sens. Actuators B: Chem. 313(2020) 128015. doi: 10.1016/j.snb.2020.128015
7. [7]
  H.X. Wu, S.C. Alexander, S.J. Jin, N.K. Devaraj, J. Am. Chem. Soc. 138(2016) 11429-11432. doi: 10.1021/jacs.6b01625
8. [8]
  Y.H. He, M. Tsutsui, R.H. Scheicher, X.S. Miao, M. Taniguchi, ACS Sens. 1(2016) 807-816. doi: 10.1021/acssensors.6b00176
9. [9]
  K. Deng, L. Wang, Q. Xia, R. Liu, J. Qu, Talanta 192(2019) 212-219. doi: 10.1016/j.talanta.2018.09.022
10. [10]
  W.J. Song, R.L. Strack, N. Svensen, S.R. Jaffrey, J. Am. Chem. Soc. 136(2014) 1198-1201. doi: 10.1021/ja410819x
11. [11]
  C.Y. Cao, P. Wei, R.H. Li, et al., ACS Sens. 4(2019) 1409-1416. doi: 10.1021/acssensors.9b00464
12. [12]
  X.J. Chen, D.S. Zhang, N. Su, et al., Nat. Biotechnol. 37(2019) 1287-1293. doi: 10.1038/s41587-019-0249-1
13. [13]
  J.H. Wu, S. Zaccara, D. Khuperkar, et al., Nat. Methods 16(2019) 862-865. doi: 10.1038/s41592-019-0531-7
14. [14]
  H.M. Wang, Z.Q.Q. Feng, W.Y. Tan, B. Xu, Bioconjugate Chem. 30(2019) 2528-2532. doi: 10.1021/acs.bioconjchem.9b00524
15. [15]
  C. Liu, X. Zhang, X. Han, et al., ACS Appl. Bio Mater. 3(2020) 1934-1943.
16. [16]
  X.W. Hua, Y.W. Bao, F.G. Wee, ACS Appl. Mater. Interfaces 10(2018) 10664-10677. doi: 10.1021/acsami.7b19549
17. [17]
  H. Liu, J. Yang, Z. Li, et al., Anal. Chem. 91(2019) 9259-9265. doi: 10.1021/acs.analchem.9b02147
18. [18]
  J. Shelton, X. Lu, J.A. Hollenbaugh, et al., Chem. Rev. 116(2016) 14379-14455. doi: 10.1021/acs.chemrev.6b00209
19. [19]
  G. Mata, O.P. Schmidt, N.W. Luedtke, Chem. Commun. 52(2016) 4718-4721. doi: 10.1039/C5CC09552B
20. [20]
  W. Xu, K.M. Chan, E.T. Kool, Nat. Chem. 9(2017) 1043-1055. doi: 10.1038/nchem.2859
21. [21]
  K. Nguyen, M. Fazio, M. Kubota, et al., J. Am. Chem. Soc. 139(2017) 2148-2151. doi: 10.1021/jacs.6b11401
22. [22]
  O.A. Zasedateleva, V.A. Vasiliskov, S.A. Surzhikov, et al., Nucleic Acids Res. 46(2018) 17. doi: 10.1093/nar/gky247
23. [23]
  Y.X. Hu, X.T. Hao, L. Xu, et al., J. Am. Chem. Soc. 142(2020) 6285-6294. doi: 10.1021/jacs.0c00698
24. [24]
  S.W. Wang, H. Chen, J. Liu, C.J. Chen, B. Liu, Adv. Funct. Mater. 30(2020) 2002546. doi: 10.1002/adfm.202002546
25. [25]
  Z. Zhao, H.K. Zhang, J.W.Y. Lam, B.Z. Tang, Angew Chem. Int. Ed. 59(2020) 9888-9907. doi: 10.1002/anie.201916729
26. [26]
  W. Zhu, M.M. Kang, Q. Wu, et al., Adv. Funct. Mater. 30(2020) 2007026.
27. [27]
  W.H. Liang, Y. Zhao, W.Z. Huang, et al., Theranostics 9(2019) 2056-2070. doi: 10.7150/thno.28119
28. [28]
  C.D. Drenberg, A.A. Gibson, S.B. Pounds, et al., Cancer Res. 77(2017) 2102-2111. doi: 10.1158/0008-5472.CAN-16-2548
29. [29]
  Z. Zawada, A. Tatar, P. Mocilac, M. Budesinsky, T. Kraus, Angew. Chem. Int. Ed. 57(2018) 9891-9895. doi: 10.1002/anie.201801306
30. [30]
  Z.K. Wang, J.Y. Nie, W. Qin, Q.L. Hu, B.Z. Tang, Nat. Commun. 7(2016) 12033. doi: 10.1038/ncomms12033
31. [31]
  X. Zhao, L. Zhao, Q. Xiao, H. Xiong, Chin. Chem. Lett. (2020), doi:http://dx.doi.org/10.1016/j.cclet.2020.10.008. doi: 10.1016/j.cclet.2020.10.008
1. [1]
  Junjie Wang , Yan Wang , Zhengdong Li , Changqiang Xie , Musammir Khan , Xingzhou Peng , Fabiao Yu . Triphenylamine-AIEgens photoactive materials for cancer theranostics. Chinese Chemical Letters, 2024, 35(6): 108934-. doi: 10.1016/j.cclet.2023.108934
2. [2]
  Xinqiong Li , Guocheng Rao , Xi Peng , Chan Yang , Yanjing Zhang , Yan Tian , Xianghui Fu , Jia Geng . Direct detection of C9orf72 hexanucleotide repeat expansions by nanopore biosensor. Chinese Chemical Letters, 2024, 35(5): 109419-. doi: 10.1016/j.cclet.2023.109419
3. [3]
  Boran Cheng , Lei Cao , Chen Li , Fang-Yi Huo , Qian-Fang Meng , Ganglin Tong , Xuan Wu , Lin-Lin Bu , Lang Rao , Shubin Wang . Fluorine-doped carbon quantum dots with deep-red emission for hypochlorite determination and cancer cell imaging. Chinese Chemical Letters, 2024, 35(6): 108969-. doi: 10.1016/j.cclet.2023.108969
4. [4]
  Zhixue Liu , Haiqi Chen , Lijuan Guo , Xinyao Sun , Zhi-Yuan Zhang , Junyi Chen , Ming Dong , Chunju Li . Luminescent terphen[3]arene sulfate-activated FRET assemblies for cell imaging. Chinese Chemical Letters, 2024, 35(9): 109666-. doi: 10.1016/j.cclet.2024.109666
5. [5]
  Qian Ren , Xue Dai , Ran Cen , Yang Luo , Mingyang Li , Ziyun Zhang , Qinghong Bai , Zhu Tao , Xin Xiao . A cucurbit[8]uril-based supramolecular phosphorescent assembly: Cell imaging and sensing of amino acids in aqueous solution. Chinese Chemical Letters, 2024, 35(12): 110022-. doi: 10.1016/j.cclet.2024.110022
6. [6]
  Panpan Wang , Hongbao Fang , Mengmeng Wang , Guandong Zhang , Na Xu , Yan Su , Hongke Liu , Zhi Su . A mitochondria targeting Ir(III) complex triggers ferroptosis and autophagy for cancer therapy: A case of aggregation enhanced PDT strategy for metal complexes. Chinese Chemical Letters, 2025, 36(1): 110099-. doi: 10.1016/j.cclet.2024.110099
7. [7]
  Jia-Mei Qin , Xue Li , Wei Lang , Fu-Hao Zhang , Qian-Yong Cao . An AIEgen nano-assembly for simultaneous detection of ATP and H₂S. Chinese Chemical Letters, 2024, 35(6): 108925-. doi: 10.1016/j.cclet.2023.108925
8. [8]
  Chuan-Zhi Ni , Ruo-Ming Li , Fang-Qi Zhang , Qu-Ao-Wei Li , Yuan-Yuan Zhu , Jie Zeng , Shuang-Xi Gu . A chiral fluorescent probe for molecular recognition of basic amino acids in solutions and cells. Chinese Chemical Letters, 2024, 35(10): 109862-. doi: 10.1016/j.cclet.2024.109862
9. [9]
  Brandon Bishop , Shaofeng Huang , Hongxuan Chen , Haijia Yu , Hai Long , Jingshi Shen , Wei Zhang . Artificial transmembrane channel constructed from shape-persistent covalent organic molecular cages capable of ion and small molecule transport. Chinese Chemical Letters, 2024, 35(11): 109966-. doi: 10.1016/j.cclet.2024.109966
10. [10]
  Xu Qu , Pengzhao Wu , Kaixuan Duan , Guangwei Wang , Liang-Liang Gao , Yuan Guo , Jianjian Zhang , Donglei Shi . Self-calibrating probes constructed on a unique dual-emissive fluorescence platform for the precise tracking of cellular senescence. Chinese Chemical Letters, 2024, 35(12): 109681-. doi: 10.1016/j.cclet.2024.109681
11. [11]
  Kun-Heng Li , Hong-Yang Zhao , Dan-Dan Wang , Ming-Hui Qi , Zi-Jian Xu , Jia-Mi Li , Zhi-Li Zhang , Shi-Wen Huang . Mitochondria-targeted nano-AIEgens as a powerful inducer for evoking immunogenic cell death. Chinese Chemical Letters, 2024, 35(5): 108882-. doi: 10.1016/j.cclet.2023.108882
12. [12]
  Meiqing Yang , Lu Wang , Haozi Lu , Yaocheng Yang , Song Liu . Recent Advances of Functional Nanomaterials for Screen-Printed Photoelectrochemical Biosensors. Acta Physico-Chimica Sinica, 2025, 41(2): 100018-. doi: 10.3866/PKU.WHXB202310046
13. [13]
  Gaowa Xing , Yuting Shang , Xiaorui Wang , Zengnan Wu , Qiang Zhang , Jiebing Ai , Qiaosheng Pu , Ling Lin . A microfluidic biosensor for multiplex immunoassay of foodborne pathogens agitated by programmed audio signals. Chinese Chemical Letters, 2024, 35(10): 109491-. doi: 10.1016/j.cclet.2024.109491
14. [14]
  Yuxin Xiao , Xiaowei Wang , Yutong Yin , Fangchao Yin , Jinchao Li , Zhiyuan Hou , Mashooq Khan , Rusong Zhao , Wenli Wu , Qiongzheng Hu . Distance-based lateral flow biosensor for the quantitative detection of bacterial endotoxin. Chinese Chemical Letters, 2024, 35(12): 109718-. doi: 10.1016/j.cclet.2024.109718
15. [15]
  Lixian Fu , Yiyun Tan , Yue Ding , Weixia Qing , Yong Wang . Water–soluble and polarity–sensitive near–infrared fluorescent probe for long–time specific cancer cell membranes imaging and C. Elegans label. Chinese Chemical Letters, 2024, 35(4): 108886-. doi: 10.1016/j.cclet.2023.108886
16. [16]
  Jianqiu Li , Yi Zhang , Songen Liu , Jie Niu , Rong Zhang , Yong Chen , Yu Liu . Cucurbit[8]uril-based non-covalent heterodimer realized NIR cell imaging through topological transformation from nanowire to nanorod. Chinese Chemical Letters, 2024, 35(10): 109645-. doi: 10.1016/j.cclet.2024.109645
17. [17]
  Caixia Zhu , Qing Hong , Kaiyuan Wang , Yanfei Shen , Songqin Liu , Yuanjian Zhang . Single nanozyme-based colorimetric biosensor for dopamine with enhanced selectivity via reactivity of oxidation intermediates. Chinese Chemical Letters, 2024, 35(10): 109560-. doi: 10.1016/j.cclet.2024.109560
18. [18]
  Qiang Li , Jiangbo Fan , Hongkai Mu , Lin Chen , Yongzhen Yang , Shiping Yu . Nucleus-targeting orange-emissive carbon dots delivery adriamycin for enhanced anti-liver cancer therapy. Chinese Chemical Letters, 2024, 35(6): 108947-. doi: 10.1016/j.cclet.2023.108947
19. [19]
  Yunan Yuan , Zhimin Luo , Jie Chen , Chaoliang He , Kai Hao , Huayu Tian . Constructing thermoresponsive PNIPAM-based microcarriers for cell culture and enzyme-free cell harvesting. Chinese Chemical Letters, 2024, 35(7): 109549-. doi: 10.1016/j.cclet.2024.109549
20. [20]
  Weiyu Chen , Zenghui Li , Chenguang Zhao , Lisha Zha , Junfeng Shi , Dan Yuan . Enzyme-modulate conformational changes in amphiphile peptide for selectively cell delivery. Chinese Chemical Letters, 2024, 35(12): 109628-. doi: 10.1016/j.cclet.2024.109628

Get Citation

PDF

XML

Metrics

PDF Downloads(11)
Abstract views(847)
HTML views(105)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142