Citation: Guangrong Zou, Kaiyuan Zhang, Wei Yang, Chaoxing Liu, Zhentian Fang, Xiang Zhou. 5-Formyluracil targeted biochemical reactions with proteins inhibit DNA replication, induce mutations and interference gene expression in living cells[J]. Chinese Chemical Letters, ;2021, 32(10): 3252-3256. doi: 10.1016/j.cclet.2021.02.036 shu

5-Formyluracil targeted biochemical reactions with proteins inhibit DNA replication, induce mutations and interference gene expression in living cells

College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, The Institute for Advanced Studies, Hubei Province Key Laboratory of Allergy and Immunology Wuhan University, Wuhan 430072, China

xzhou@whu.edu.cn

Received Date: 14 January 2021
Revised Date: 29 January 2021
Accepted Date: 17 February 2021
Available Online: 19 February 2021

Figures(6)

Covalent DNA–protein cross-links are toxic DNA lesions that interfere with essential biological processes, which can cause serious biological consequences, such as genomic instability and protein misexpression. 5-Formyluracil (5fU) as an important modification in DNA, which is mainly from oxidative damage, exists in a variety of cells and tissues. We have reported that 5fU mediated DNA–protein conjugates could exist in human cells [Zhou et al. CCS Chem. 2 (2020) 54-63]. We now aimed to explore its potential biological effects in vitro and in vivo. In this paper, we firstly reported that 5fU intermediated DNA–peptide or DNA–protein conjugates (both were called DPCs) could inhibit different polymerases bypass or cause mutations. Then we further investigated the functional impacts caused by 5fU-mediated DPCs, which appeared in different gene expression components [in the promoter sequence or 5′-untranslated regions (UTR)]. These results together may contribute to a broader understanding of DNA–protein interactions as well as the biological functions associated with 5fU.
- 5-Formyluracil,
- Biochemical reaction,
- DNA replication,
- Mutation,
- Gene expression
1. [1]
  C. Luo, P. Hajkova, J.R. Ecker, Science 361(2018) 1336-1340. doi: 10.1126/science.aat6806
2. [2]
  A. Hofer, Z.J. Liu, S. Balasubramanian, J. Am. Chem. Soc. 141(2019) 6420-6429. doi: 10.1021/jacs.9b01915
3. [3]
  M.J. Booth, E.A. Raiber, S. Balasubramanian, Chem. Rev. 115(2015) 2240-2254. doi: 10.1021/cr5002904
4. [4]
  T. Carell, M.Q. Kurz, M. Müller, et al., Angew. Chem. Int. Ed. 57(2018) 4296-4312. doi: 10.1002/anie.201708228
5. [5]
  W. Hirose, K. Sato, A. Matsuda, Angew. Chem. Int. Ed. 49(2010) 8392-8394. doi: 10.1002/anie.201004087
6. [6]
  C. Liu, Y. Chen, Y. Wang, et al., Nano Res. 10(2017) 2449-2458. doi: 10.1007/s12274-017-1445-2
7. [7]
  H.P. Jiang, T. Liu, N. Guo, et al., Anal. Chim. Acta 981(2017) 1-10. doi: 10.1016/j.aca.2017.06.009
8. [8]
  N.Y. Tretyakova, A. Groehler, S. Ji, Acc. Chem. Res. 48(2015) 1631-1644. doi: 10.1021/acs.accounts.5b00056
9. [9]
  T. Nakano, H. Terato, K. Asagoshi, et al., J. Biol. Chem. 278(2003) 25264-25272. doi: 10.1074/jbc.M212847200
10. [10]
  H. Ide, M.I. Shoulkamy, T. Nakano, et al., Mutat. Res. Fund. Mol. Mech. Mutagen. 711(2011) 113-122. doi: 10.1016/j.mrfmmm.2010.12.007
11. [11]
  A.M. Sanchez, I.G. Minko, A.J. Kurtz, et al., Chem. Res. Toxicol. 16(2003) 1019-1028. doi: 10.1021/tx034066u
12. [12]
  M. Dizdaroglu, E. Gajewski, Cancer Res. 49(1989) 3463-3467.
13. [13]
  P. Pande, S. Ji, S. Mukherjee, et al., Chem. Res. Toxicol. 30(2017) 669-677. doi: 10.1021/acs.chemrestox.6b00397
14. [14]
  S. Wickramaratne, E.J. Boldry, C. Buehler, et al., J. Biol. Chem. 290(2015) 775-787. doi: 10.1074/jbc.M114.613638
15. [15]
  E.A. Raiber, G. Portella, S. Martínez Cuesta, et al., Nat. Chem. 10(2018) 1258-1266. doi: 10.1038/s41557-018-0149-x
16. [16]
  Z.J. Liu, S. Martínez Cuesta, P. van Delft, et al., Nat. Chem. 11(2019) 629-637. doi: 10.1038/s41557-019-0279-9
17. [17]
  R.E. Hardisty, F. Kawasaki, A.B. Sahakyan, et al., J. Am. Chem. Soc. 137(2015) 9270-9272. doi: 10.1021/jacs.5b03730
18. [18]
  G. Zou, C. Liu, W. Zeng, et al., CCS Chem. 2(2020) 54-63. doi: 10.31635/ccschem.020.201900078
19. [19]
  S. Ji, H. Shao, Q. Han, et al., Angew. Chem. Int. Ed. 56(2017) 14130-14134. doi: 10.1002/anie.201708286
20. [20]
  J.B. Stafford, R.L. Eoff, A. Kozekova, et al., Biochemistry 48(2009) 471-480. doi: 10.1021/bi801591a
21. [21]
  C.K. Sen, L. Packer, FASEB J. 10(1996) 709-720. doi: 10.1096/fasebj.10.7.8635688
22. [22]
  H. Sies, Exp. Physiol. 82(1997) 291-295. doi: 10.1113/expphysiol.1997.sp004024
23. [23]
  R. Ghosh, D.L. Mitchell, Nucleic Acids Res. 27(1999) 3213-3218. doi: 10.1093/nar/27.15.3213
1. [1]
  Neng Shi , Haonan Jia , Jixiang Zhang , Pengyu Lu , Chenglong Cai , Yixin Zhang , Liqiang Zhang , Nongyue He , Weiran Zhu , Yan Cai , Zhangqi Feng , Ting Wang . Accurate expression of neck motion signal by piezoelectric sensor data analysis. Chinese Chemical Letters, 2024, 35(9): 109302-. doi: 10.1016/j.cclet.2023.109302
2. [2]
  Zhen Liu , Zhi-Yuan Ren , Chen Yang , Xiangyi Shao , Li Chen , Xin Li . Asymmetric alkenylation reaction of benzoxazinones with diarylethylenes catalyzed by B(C₆F₅)₃/chiral phosphoric acid. Chinese Chemical Letters, 2024, 35(5): 108939-. doi: 10.1016/j.cclet.2023.108939
3. [3]
  Chang Liu , Tao Wu , Lijiao Deng , Xuzi Li , Xin Fu , Shuzhen Liao , Wenjie Ma , Guoqiang Zou , Hai Yang . Programmed DNA walkers for biosensors. Chinese Chemical Letters, 2024, 35(9): 109307-. doi: 10.1016/j.cclet.2023.109307
4. [4]
  Ling Yang , Min Ren , Jie Wang , Liming He , Shanshan Wu , Shuai Yang , Wei Zhao , Hao Cheng , Xiaoming Zhou , Maling Gou . A non-viral gene therapy for melanoma by staphylococcal enterotoxin A. Chinese Chemical Letters, 2024, 35(5): 108822-. doi: 10.1016/j.cclet.2023.108822
5. [5]
  Mengxiang Zhu , Tao Ding , Yunzhang Li , Yuanjie Peng , Ruiping Liu , Quan Zou , Leilei Yang , Shenglei Sun , Pin Zhou , Guosheng Shi , Dongting Yue . Graphene controlled solid-state growth of oxygen vacancies riched V₂O₅ catalyst to highly activate Fenton-like reaction. Chinese Chemical Letters, 2024, 35(12): 109833-. doi: 10.1016/j.cclet.2024.109833
6. [6]
  Yan Liu , Yang Wang , Jiayi Zhu , Xuxian Su , Xudong Lin , Liang Xu , Xiwen Xing . Employing pH-responsive RNA triplex to control CRISPR/Cas9-mediated gene manipulation in mammalian cells. Chinese Chemical Letters, 2024, 35(9): 109427-. doi: 10.1016/j.cclet.2023.109427
7. [7]
  Jia-Li Xie , Tian-Jin Xie , Yu-Jie Luo , Kai Mao , Cheng-Zhi Huang , Yuan-Fang Li , Shu-Jun Zhen . Octopus-like DNA nanostructure coupled with graphene oxide enhanced fluorescence anisotropy for hepatitis B virus DNA detection. Chinese Chemical Letters, 2024, 35(6): 109137-. doi: 10.1016/j.cclet.2023.109137
8. [8]
  Zhili Li , Qijun Wo , Dongdong Huang , Dezhong Zhou , Lei Guo , Yeqing Mao . Improving gene transfection efficiency of highly branched poly(β-amino ester)s through the in-situ conversion of inactive terminal groups. Chinese Chemical Letters, 2024, 35(8): 109737-. doi: 10.1016/j.cclet.2024.109737
9. [9]
  Yang Qin , Jiangtian Li , Xuehao Zhang , Kaixuan Wan , Heao Zhang , Feiyang Huang , Limei Wang , Hongxun Wang , Longjie Li , Xianjin Xiao . Toeless and reversible DNA strand displacement based on Hoogsteen-bond triplex. Chinese Chemical Letters, 2024, 35(5): 108826-. doi: 10.1016/j.cclet.2023.108826
10. [10]
  Xiaohong Wen , Mei Yang , Lie Li , Mingmin Huang , Wei Cui , Suping Li , Haiyan Chen , Chen Li , Qiuping Guo . Enzymatically controlled DNA tetrahedron nanoprobes for specific imaging of ATP in tumor. Chinese Chemical Letters, 2024, 35(8): 109291-. doi: 10.1016/j.cclet.2023.109291
11. [11]
  Jingwen Zhao , Jianpu Tang , Zhen Cui , Limin Liu , Dayong Yang , Chi Yao . A DNA micro-complex containing polyaptamer for exosome separation and wound healing. Chinese Chemical Letters, 2024, 35(9): 109303-. doi: 10.1016/j.cclet.2023.109303
12. [12]
  Zhongyu Wang , Lijun Wang , Huaixin Zhao . DNA-based nanosystems to generate reactive oxygen species for nanomedicine. Chinese Chemical Letters, 2024, 35(11): 109637-. doi: 10.1016/j.cclet.2024.109637
13. [13]
  Jiangshan Xu , Weifei Zhang , Zhengwen Cai , Yong Li , Long Bai , Shaojingya Gao , Qiang Sun , Yunfeng Lin . Tetrahedron DNA nanostructure/iron-based nanomaterials for combined tumor therapy. Chinese Chemical Letters, 2024, 35(11): 109620-. doi: 10.1016/j.cclet.2024.109620
14. [14]
  Tian Feng , Yun-Ling Gao , Di Hu , Ke-Yu Yuan , Shu-Yi Gu , Yao-Hua Gu , Si-Yu Yu , Jun Xiong , Yu-Qi Feng , Jie Wang , Bi-Feng Yuan . Chronic sleep deprivation induces alterations in DNA and RNA modifications by liquid chromatography-mass spectrometry analysis. Chinese Chemical Letters, 2024, 35(8): 109259-. doi: 10.1016/j.cclet.2023.109259
15. [15]
  Zhe-Han Yang , Jie Yin , Lei Xin , Yuanfang Li , Yijie Huang , Ruo Yuan , Ying Zhuo . Research advancement of DNA-based intelligent hydrogels: Manufacture, characteristics, application of disease diagnosis and treatment. Chinese Chemical Letters, 2024, 35(10): 109558-. doi: 10.1016/j.cclet.2024.109558
16. [16]
  Xiaofen GUAN , Yating LIU , Jia LI , Yiwen HU , Haiyuan DING , Yuanjing SHI , Zhiqiang WANG , Wenmin WANG . Synthesis, crystal structure, and DNA-binding of binuclear lanthanide complexes based on a multidentate Schiff base ligand. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2486-2496. doi: 10.11862/CJIC.20240122
17. [17]
  Yanfei Liu , Yaqin Hu , Yifu Tan , Qiwen Chen , Zhenbao Liu . Tumor acidic microenvironment activatable DNA nanostructure for precise cancer cell targeting and inhibition. Chinese Chemical Letters, 2025, 36(1): 110289-. doi: 10.1016/j.cclet.2024.110289
18. [18]
  Fanghua Zhang , Yuyan Li , Hongyan Zhang , Wendong Liu , Zhe Hao , Mingzheng Shao , Ruizhong Zhang , Xiyan Li , Libing Zhang . Logically integrating exo/endogenous gated DNA trackers for precise microRNA imaging via synergistic manipulation. Chinese Chemical Letters, 2025, 36(1): 109848-. doi: 10.1016/j.cclet.2024.109848
19. [19]
  Kun Liu , Yulin Cong , Xiongfeng Luo , Meicun Yao , Zhiyong Xie , Hao Li . Utilizing bivalent aptamers as first DNA agonist to activate RTKs heterodimer of different families. Chinese Chemical Letters, 2025, 36(1): 109839-. doi: 10.1016/j.cclet.2024.109839
20. [20]
  Yao HUANG , Yingshu WU , Zhichun BAO , Yue HUANG , Shangfeng TANG , Ruixue LIU , Yancheng LIU , Hong LIANG . Copper complexes of anthrahydrazone bearing pyridyl side chain: Synthesis, crystal structure, anticancer activity, and DNA binding. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 213-224. doi: 10.11862/CJIC.20240359

Get Citation

PDF

XML

Metrics

PDF Downloads(4)
Abstract views(617)
HTML views(45)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142