Citation: Yuhui Zhang, Lijuan Wang, Jie Wang, Siqintana Xin, Xianliang Sheng. Enzyme-responsive polysaccharide supramolecular nanoassembly for enhanced DNA encapsulation and controlled release[J]. Chinese Chemical Letters, ;2021, 32(6): 1902-1906. doi: 10.1016/j.cclet.2021.01.032 shu

Enzyme-responsive polysaccharide supramolecular nanoassembly for enhanced DNA encapsulation and controlled release

Yuhui Zhang ^{a, 1,} ,
Lijuan Wang ^{b, 1} ,
Jie Wang ^a ,
Siqintana Xin ^a ,
Xianliang Sheng ^{a, *,}

a.
College of Science, Inner Mongolia Agricultural University, Hohhot 010018, China
b.
College of Material Science and Art Design, Inner Mongolia Agricultural University, Hohhot 010018, China

yh_zhangyh@126.com

shengxl@iccas.ac.cn

Received Date: 20 October 2020
Revised Date: 12 January 2021
Accepted Date: 18 January 2021
Available Online: 26 January 2021

Figures(5)

An enzyme-responsive polysaccharide supramolecular targeted nanoassembly was successfully constructed by the host-guest complexation of positively charged mono-(6-(tetraethylenepentamine)-6-deoxy)-β-cyclodextrin (TEPA-CD) with adamantane-grafted hyaluronic acid (HA-ADA). Possessing a series of positively charged polyamine chains, the obtained polysaccharide nanoassembly could serve as a biocompatible plasmid DNA (pDNA) container. More interestingly, the pDNA could be released from the nanoassembly through the enzymatic degradation of HA skeleton, which realized the controlled pDNA binding and release. Besides, the polysaccharide nanoassembly exhibited lower cytotoxicity than the commercial transfection reagents 25kDa bPEI (PEI_25k), accompanied by similar gene delivery effect. We believe that this work might present a convenient method for targeted, controlled gene delivery.
- Enzyme-responsive,
- Cyclodextrin,
- Hyaluronic acid,
- Gene delivery,
- Supramolecular chemistry
1. [1]
  C.O. Mellet, J.G. Fernández, J.M. Benito, Chem. Soc. Rev. 40(2011) 1586-1608. doi: 10.1039/C0CS00019A
2. [2]
  W.C. Geng, Q. Huang, Z. Xu, R. Wang, D.S. Guo, Theranostics 9(2019) 3094-3106. doi: 10.7150/thno.31914
3. [3]
  S. Roy, D.C. Zhu, W.J. Parak, N. Feliu, ACS Nano 14(2020) 8012-8023. doi: 10.1021/acsnano.9b10219
4. [4]
  H. Cabral, K. Miyata, K. Osada, K. Kataoka, Chem. Rev. 118(2020) 6844-6892.
5. [5]
  A.B. Cook, S. Perrier, Adv. Funct. Mater. 30(2020) 1901001. doi: 10.1002/adfm.201901001
6. [6]
  Y.Y. Cheng, X.Y. Jiao, W.P. Fan, et al., Biomaterials 256(2020) 120191. doi: 10.1016/j.biomaterials.2020.120191
7. [7]
  Y. Lin, J.H. Wu, W.H. Gu, et al., Adv. Sci. 5(2018) 1700611. doi: 10.1002/advs.201700611
8. [8]
  B.D. Rodrigues, A. Banerjee, T. Kanekiyo, J. Singh, Int. J. Pharm. 566(2019) 717-730. doi: 10.1016/j.ijpharm.2019.06.026
9. [9]
  M. Liu, Y. Peng, Y.B. Nie, et al., Acta Biomater. 110(2020) 242-253. doi: 10.1016/j.actbio.2020.04.041
10. [10]
  X.S. Li, J.Y. Han, X. Wang, et al., Mater. Chem. Front. 3(2019) 103-110. doi: 10.1039/C8QM00509E
11. [11]
  Z.X. Zhou, X.R. Liu, D.C. Zhu, et al., Adv. Drug Delivery Rev. 115(2017) 115-154. doi: 10.1016/j.addr.2017.07.021
12. [12]
  T.V. Mashel, Y.V. Tarakanchikova, A.R. Muslimov, et al., Biomaterials258(2020) 120282. doi: 10.1016/j.biomaterials.2020.120282
13. [13]
  R. Mohammadinejad, A. Dehshahri, V.S. Madamsetty, et al., J. Control. Release 325(2020) 249-275. doi: 10.1016/j.jconrel.2020.06.038
14. [14]
  K. Chatterjee, S. Sarkar, K.J. Rao, S. Paria, Adv. Colloid Interface Sci. 209(2014) 8-39. doi: 10.1016/j.cis.2013.12.008
15. [15]
  T.X. Xiao, W.W. Zhong, L.X. Xu, et al., Org. Biomol. Chem. 17(2019) 1336-1350. doi: 10.1039/C8OB03095B
16. [16]
  C.P. Jiang, Z.T. Qi, H.B. Jia, et al., Biomacromolecules 20(2019) 478-489. doi: 10.1021/acs.biomac.8b01395
17. [17]
  X. Jin, L.J. Zhu, B. Xue, X.Y. Zhu, D.Y. Yan, Natl. Sci. Rev. 6(2019) 1128-1137. doi: 10.1093/nsr/nwz018
18. [18]
  J.Y. Chen, Y.D. Zhang, Z. Meng, et al., Chem. Sci. 11(2020) 6275-6282. doi: 10.1039/D0SC01756F
19. [19]
  Q. Zhang, C.Y. Shi, D.H. Qu, et al., Sci. Adv. 4(2018) eaat8192. doi: 10.1126/sciadv.aat8192
20. [20]
  M.X. Wu, H.J. Yan, J. Gao, et al., ACS Appl. Mater. Interfaces 10(2018) 34655-34663. doi: 10.1021/acsami.8b13758
21. [21]
  P.Y. Li, Y. Chen, Y. Liu, Chin. Chem. Lett. 30(2019) 1190-1197. doi: 10.1016/j.cclet.2019.03.035
22. [22]
  Y.M. Zhang, Y.H. Liu, Y. Liu, Adv. Mater. 32(2020) 1806158. doi: 10.1002/adma.201806158
23. [23]
  Q.D. Hu, G.P. Tang, P.K. Chu, Acc. Chem. Res. 47(2014) 2017-2025. doi: 10.1021/ar500055s
24. [24]
  Y.T. Wen, H.Z. Bai, J.L. Zhu, et al., Sci. Adv. 6(2020) eabc2148. doi: 10.1126/sciadv.abc2148
25. [25]
  M.A. Raquel, G.S. Laura, W. Verboom, J. Huskens, J. Mater. Chem. B5(2017)36-52. doi: 10.1039/C6TB02776H
26. [26]
  K.I. Assaf, J. Holub, E. Bernhardt, et al., ChemPhysChem 21(2020) 971-976. doi: 10.1002/cphc.201901225
27. [27]
  L. Liang, Y. Chen, X.M. Chen, Y. Zhang, Y. Liu, Chin. Chem. Lett. 29(2018) 989-991. doi: 10.1016/j.cclet.2017.12.022
28. [28]
  L. Zhang, Y.M. Zhang, G.X. Liu, Y. Liu, Chin. Chem. Lett. 30(2019) 120-122. doi: 10.1016/j.cclet.2018.04.028
29. [29]
  Q. Zhao, Y. Chen, Y. Liu, Chin. Chem. Lett. 29(2018) 84-86. doi: 10.1016/j.cclet.2017.07.024
30. [30]
  Y.J. Zhang, T. Sun, C. Jiang, Acta Pharm. Sin. B 8(2018) 34-50. doi: 10.1016/j.apsb.2017.11.005
31. [31]
  W. Zhong, L. Pang, H.H. Feng, et al., Carbohydr. Polym. 238(2020) 116204. doi: 10.1016/j.carbpol.2020.116204
32. [32]
  N. Zheng, D. Xie, C.S. Wang, et al., ACS Appl. Mater. Interfaces 11(2019) 44007-44017. doi: 10.1021/acsami.9b19546
33. [33]
  X. Wang, M.H. Li, Y.H. Hou, et al., Adv. Funct. Mater. 30(2020) 2000229. doi: 10.1002/adfm.202000229
34. [34]
  T.L. Nascimento, H. Hillaireau, J. Vergnaud, E. Fattal, Nanomedicine 11(2016) 1865-1887. doi: 10.2217/nnm-2016-5000
35. [35]
  Y.H. Zhang, Y.M. Zhang, J. Yu, J. Wang, Y. Liu, Chem. Commun. 55(2019) 1164-1167. doi: 10.1039/C8CC09956A
36. [36]
  K.Y. Choi, O.F. Silvestre, X.L. Huang, et al., Nat. Protoc. 9(2014) 1900-1915. doi: 10.1038/nprot.2014.128
37. [37]
  F.Q. Li, Q.L. Yu, Y.H. Liu, et al., Chem. Commun. 56(2020) 3907-3910. doi: 10.1039/D0CC00629G
38. [38]
  X. Ma, Y.L. Zhao, Chem. Rev. 115(2015) 7794-7839. doi: 10.1021/cr500392w
39. [39]
  H.Q. Song, W.T. Pan, R.Q. Li, et al., Small 14(2018) 1703152. doi: 10.1002/smll.201703152
40. [40]
  Y. Yang, Y.M. Zhang, Y. Chen, J.T. Chen, Y. Liu, Sci. Rep. 6(2016) 19212. doi: 10.1038/srep19212
41. [41]
  Y. Bai, C.P. Liu, D. Chen, et al., Carbohydr. Polym. 246(2020) 116654. doi: 10.1016/j.carbpol.2020.116654
42. [42]
  H.P. Zhang, J. Liu, Q.X. Chen, P. Mi, J. Control. Release 320(2020) 314-327. doi: 10.1016/j.jconrel.2020.01.026
43. [43]
  X.Y. Xu, Z.S. Zeng, J. Chen, et al., Chem. Eng. J. 390(2020) 124628. doi: 10.1016/j.cej.2020.124628
44. [44]
  K.Y. Choi, H.S. Han, E.S. Lee, et al., Adv. Mater. 31(2019) 1803549. doi: 10.1002/adma.201803549
45. [45]
  L. Hou, Y.L. Zhang, X.M. Yang, et al., ACS Appl. Mater. Interfaces 11(2019) 255-268. doi: 10.1021/acsami.8b17750
46. [46]
  Y.H. Zhang, Y.M. Zhang, X.L. Sheng, J. Wang, Y. Liu, Chem. Commun. 56(2020) 1042-1045. doi: 10.1039/C9CC08491F
47. [47]
  P. Singh, L. Wu, X.H. Ren, et al., Int. J. Pharm. 586(2020) 119542. doi: 10.1016/j.ijpharm.2020.119542
48. [48]
  Z. Tan, Y. Jiang, W. Zhang, et al., J. Am. Chem. Soc. 141(2019) 15804-15817. doi: 10.1021/jacs.9b06218
49. [49]
  P. Ayaz, B.J. Xu, X.S. Zhang, et al., Appl. Surf. Sci. 527(2020) 146806. doi: 10.1016/j.apsusc.2020.146806
50. [50]
  Y.M. Zhang, Y. Cao, Y. Yang, J.T. Chen, Y. Liu, Chem. Commun. 50(2014) 13066-13069. doi: 10.1039/C4CC04533E
51. [51]
  J.C. Beeson, A.W. Czarnik, Bioorg. Med. Chem. 2(1994) 297-303. doi: 10.1016/S0968-0896(00)82172-0
52. [52]
  C. Kinnear, T.L. Moore, R.L. Laura, R.R. Barbara, P.F. Alke, Chem. Rev. 117(2017) 11476-11521. doi: 10.1021/acs.chemrev.7b00194
1. [1]
  Rui Wang , Yang Liang , Julius Rebek Jr. , Yang Yu . Stabilization and detection of labile reaction intermediates in supramolecular containers. Chinese Chemical Letters, 2024, 35(6): 109228-. doi: 10.1016/j.cclet.2023.109228
2. [2]
  Chao Zhang , Ai-Feng Liu , Shihui Li , Fang-Yuan Chen , Jun-Tao Zhang , Fang-Xing Zeng , Hui-Chuan Feng , Ping Wang , Wen-Chao Geng , Chuan-Rui Ma , Dong-Sheng Guo . A supramolecular formulation of icariin@sulfonatoazocalixarene for hypoxia-targeted osteoarthritis therapy. Chinese Chemical Letters, 2025, 36(1): 109752-. doi: 10.1016/j.cclet.2024.109752
3. [3]
  Jie Yang , Xin-Yue Lou , Dihua Dai , Jingwei Shi , Ying-Wei Yang . Desymmetrized pillar[8]arenes: High-yield synthesis, functionalization, and host-guest chemistry. Chinese Chemical Letters, 2025, 36(1): 109818-. doi: 10.1016/j.cclet.2024.109818
4. [4]
  Zhenzhu Wang , Chenglong Liu , Yunpeng Ge , Wencan Li , Chenyang Zhang , Bing Yang , Shizhong Mao , Zeyuan Dong . Differentiated self-assembly through orthogonal noncovalent interactions towards the synthesis of two-dimensional woven supramolecular polymers. Chinese Chemical Letters, 2024, 35(5): 109127-. doi: 10.1016/j.cclet.2023.109127
5. [5]
  Xuanyu Wang , Zhao Gao , Wei Tian . Supramolecular confinement effect enabling light-harvesting system for photocatalytic α-oxyamination reaction. Chinese Chemical Letters, 2024, 35(11): 109757-. doi: 10.1016/j.cclet.2024.109757
6. [6]
  Kang Wei , Jiayu Li , Wen Zhang , Bing Yuan , Ming-De Li , Pingwu Du . A strained π-extended [10]cycloparaphenylene carbon nanoring. Chinese Chemical Letters, 2024, 35(5): 109055-. doi: 10.1016/j.cclet.2023.109055
7. [7]
  Junying Zhang , Ruochen Li , Haihua Wang , Wenbing Kang , Xing-Dong Xu . Photo-induced tunable luminescence from an aggregated amphiphilic ethylene-pyrene derivative in aqueous media. Chinese Chemical Letters, 2024, 35(6): 109216-. doi: 10.1016/j.cclet.2023.109216
8. [8]
  Zhimin Sun , Xin-Hui Guo , Yue Zhao , Qing-Yu Meng , Li-Juan Xing , He-Lue Sun . Dynamically switchable porphyrin-based molecular tweezer for on−off fullerene recognition. Chinese Chemical Letters, 2024, 35(6): 109162-. doi: 10.1016/j.cclet.2023.109162
9. [9]
  Zixi Zou , Jingyuan Wang , Yian Sun , Qian Wang , Da-Hui Qu . Controlling molecular assembly on time scale: Time-dependent multicolor fluorescence for information encryption. Chinese Chemical Letters, 2024, 35(7): 108972-. doi: 10.1016/j.cclet.2023.108972
10. [10]
  Cheng-Da Zhao , Huan Yao , Shi-Yao Li , Fangfang Du , Li-Li Wang , Liu-Pan Yang . Amide naphthotubes: Biomimetic macrocycles for selective molecular recognition. Chinese Chemical Letters, 2024, 35(4): 108879-. doi: 10.1016/j.cclet.2023.108879
11. [11]
  Zhengzhong Zhu , Shaojun Hu , Zhi Liu , Lipeng Zhou , Chongbin Tian , Qingfu Sun . A cationic radical lanthanide organic tetrahedron with remarkable coordination enhanced radical stability. Chinese Chemical Letters, 2025, 36(2): 109641-. doi: 10.1016/j.cclet.2024.109641
12. [12]
  Wenjia Wang , Xingyue He , Xiaojie Wang , Tiantian Zhao , Osamu Muraoka , Genzoh Tanabe , Weijia Xie , Tianjiao Zhou , Lei Xing , Qingri Jin , Hulin Jiang . Glutathione-depleted cyclodextrin pseudo-polyrotaxane nanoparticles for anti-inflammatory oxaliplatin (Ⅳ) prodrug delivery and enhanced colorectal cancer therapy. Chinese Chemical Letters, 2024, 35(4): 108656-. doi: 10.1016/j.cclet.2023.108656
13. [13]
  Weiyu Chen , Zenghui Li , Chenguang Zhao , Lisha Zha , Junfeng Shi , Dan Yuan . Enzyme-modulate conformational changes in amphiphile peptide for selectively cell delivery. Chinese Chemical Letters, 2024, 35(12): 109628-. doi: 10.1016/j.cclet.2024.109628
14. [14]
  Yan Liu , Yang Wang , Jiayi Zhu , Xuxian Su , Xudong Lin , Liang Xu , Xiwen Xing . Employing pH-responsive RNA triplex to control CRISPR/Cas9-mediated gene manipulation in mammalian cells. Chinese Chemical Letters, 2024, 35(9): 109427-. doi: 10.1016/j.cclet.2023.109427
15. [15]
  Ling Zhang , Jing Kang . Turn Waste into Valuable: Preparation of High-Strength Water-Based Adhesives from Polymethylmethacrylate Wastes: a Comprehensive Chemical Experiments. University Chemistry, 2024, 39(2): 221-226. doi: 10.3866/PKU.DXHX202306075
16. [16]
  Jiarui Wu , Gengxin Wu , Yan Wang , Yingwei Yang . Crystal Engineering Based on Leaning Towerarenes. University Chemistry, 2024, 39(3): 58-62. doi: 10.3866/PKU.DXHX202304014
17. [17]
  Siwei Wang , Wei-Lei Zhou , Yong Chen . Cucurbituril and cyclodextrin co-confinement-based multilevel assembly for single-molecule phosphorescence resonance energy transfer behavior. Chinese Chemical Letters, 2024, 35(12): 110261-. doi: 10.1016/j.cclet.2024.110261
18. [18]
  Dexuan Xiao , Tianyu Chen , Tianxu Zhang , Sirong Shi , Mei Zhang , Xin Qin , Yunkun Liu , Longjiang Li , Yunfeng Lin . Transdermal treatment for malignant melanoma by aptamer-modified tetrahedral framework nucleic acid delivery of vemurafenib. Chinese Chemical Letters, 2024, 35(4): 108602-. doi: 10.1016/j.cclet.2023.108602
19. [19]
  Yunjie Dang , Yanru Feng , Xiao Chen , Chaoxing He , Shujie Wei , Dingyang Liu , Jinlong Qi , Huaxing Zhang , Shaokun Yang , Zhiyun Niu , Bai Xiang . Development of a multi-level pH-responsive lipid nanoplatform for efficient co-delivery of siRNA and small-molecule drugs in tumor treatment. Chinese Chemical Letters, 2024, 35(12): 109660-. doi: 10.1016/j.cclet.2024.109660
20. [20]
  Wei Chen , Pieter Cnudde . A minireview to ketene chemistry in zeolite catalysis. Chinese Journal of Structural Chemistry, 2024, 43(11): 100412-100412. doi: 10.1016/j.cjsc.2024.100412

Get Citation

PDF

XML

Metrics

PDF Downloads(5)
Abstract views(637)
HTML views(54)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142