Citation: Wei Lei, Fukui Shen, Nianwei Chang, Lin Niu, Yuanyuan Hou, Boli Zhang, Min Jiang, Gang Bai. Chemical proteomics reveals ligustilide targets SMAD3, inhibiting collagen synthesis in aortic endothelial cells[J]. Chinese Chemical Letters, ;2021, 32(1): 190-193. doi: 10.1016/j.cclet.2020.10.049 shu

Chemical proteomics reveals ligustilide targets SMAD3, inhibiting collagen synthesis in aortic endothelial cells

Wei Lei ^{a, b} ,
Fukui Shen ^a ,
Nianwei Chang ^b ,
Lin Niu ^b ,
Yuanyuan Hou ^a ,
Boli Zhang ^b ,
Min Jiang ^{a, *,} ,
Gang Bai ^{a, *,}

a.
State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300353, China
b.
Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China

minjiang@nankai.edu.cn

gangbai@nankai.edu.cn

Received Date: 17 September 2020
Revised Date: 12 October 2020
Accepted Date: 31 October 2020
Available Online: 3 November 2020

Figures(4)

Atherosclerosis is a persistent inflammatory state, while vascular endothelial fibrosis is one of the primary causes of atherosclerosis development. Although ligustilide (Lig) was shown to exert obvious antiatherogenic effects in previous studies, its precise mechanism has not been deeply discussed. In this paper, we designed a Lig-derived photoaffinity labelling (PAL) probe to identify potential therapeutic targets of Lig via chemical proteomics approach. Mothers against decapentaplegic homologue 3 (SMAD3), a signal transmitter of transforming growth factor-β (TGF-β) which promotes the development of vascular fibrosis, was identified as a potential target of Lig. Lig suppressed the phosphorylation and nuclear translocation of SMAD3 by blocking the interaction between SMAD3 and TGF-β receptor 1, thereby inhibiting the collagen synthesis process. Hence, developing a novel SMAD3 inhibitor may present a promising therapeutic option for preventing vascular fibrosis.
- Ligustilide,
- SMAD3,
- Collagen synthesis,
- Chemical proteomics,
- Photoaffinity labelling
1. [1]
  P. Libby, P.M. Ridker, G.K. Hansson, Nature 473(2011) 317-325. doi: 10.1038/nature10146
2. [2]
  C.P. Denton, Semin. Arthritis Rheum. 49(2019) S3-S7. doi: 10.1016/j.semarthrit.2019.09.019
3. [3]
  N.A. Shirwany, M.H. Zou, Acta Pharmacol. Sin. 31(2010) 1267-1276. doi: 10.1038/aps.2010.123
4. [4]
  E. Agabiti-Rosei, M. Heagerty, D. Rizzoni, J. Hypertens. 27(2009) 1107-1114. doi: 10.1097/HJH.0b013e328329272e
5. [5]
  L.F. Fried, Kidney Int. 74(2008) 571-576. doi: 10.1038/ki.2008.231
6. [6]
  F. Simko, Eur. J. Clin. Invest. 37(2007) 681-691. doi: 10.1111/j.1365-2362.2007.01837.x
7. [7]
  J.Y. Yang, H.H. Chen, J. Wu, et al., Chin. Herb. Med. 1(2012) 26-32.
8. [8]
  V. Bunel, M.H. Antoine, J. Nortier, et al., Toxicol. In Vitro 29(2015) 458-467. doi: 10.1016/j.tiv.2014.12.017
9. [9]
  W. Lei, J.N. Ni, X.T. Xia, et al., J. Chromatogr. B 15(2018) 220-227.
10. [10]
  Y. Zhu, Y. Zhang, X. Huang, et al., Atherosclerosis 284(2019) 110-120. doi: 10.1016/j.atherosclerosis.2019.02.010
11. [11]
  W. Lei, Y.F. Deng, X.Y. Hu, et al., Biomed. Pharmacother. 117(2019) 109074. doi: 10.1016/j.biopha.2019.109074
12. [12]
  L. Wang, Y. Sun, C. Ruan, et al., Biotechnol. Equip. 28(2014) 923-928. doi: 10.1080/13102818.2014.957487
13. [13]
  C. Zhang, X. Kong, H. Zhou, et al., Evid. Complement. Alternat. Med. 2013(2013) 291814. doi: 10.1155/2013/291814
14. [14]
  C. Ma, Z. Fu, H. Guo, et al., Biomed. Pharmacother. 112(2019) 108596. doi: 10.1016/j.biopha.2019.01.057
15. [15]
  Y. Ren, Q.S. Sun, Z.G. Yuan, et al., Chin. Chem. Lett. 30(2019) 1233-1236. doi: 10.1016/j.cclet.2019.03.029
16. [16]
  J.T. Du, J. Guo, D.W. Kang, et al., Chin. Chem. Lett. 31(2020) 1695-1708. doi: 10.1016/j.cclet.2020.03.028
17. [17]
  G.D. Hu, H.Y. Jia, L.N. Zhao, et al., Chin. Chem. Lett. 30(2019) 1704-1716. doi: 10.1016/j.cclet.2019.06.039
18. [18]
  S. Pan, H. Zhang, C. Wang, et al., Nat. Prod. Rep. 5(2016) 612-620. doi: 10.1039/C5NP00101C
19. [19]
  Y. Zhang, Z. Wang, X. Ma, et al., Acta Pharm. Sin. B 9(2019) 294-303. doi: 10.1016/j.apsb.2018.11.002
20. [20]
  M. Matthias, Matrix Biol. 28-29(2018) 106-121.
21. [21]
  R.A. Smith, J.R. Knowles, J. Am. Chem. Soc. 95(1973) 5072-5073. doi: 10.1021/ja00796a062
22. [22]
  A.J. Lusis, Nature 407(2000) 233-241. doi: 10.1038/35025203
23. [23]
  X. Sun, B. Nkennor, O. Mastikhina, et al., Semin. Cell Dev. Biol. 101(2020) 78-86. doi: 10.1016/j.semcdb.2019.10.015
24. [24]
  T.H. Lan, X.Q. Huang, H.M. Tan, et al., Cardiovasc. Pathol. 22(2013) 401-407. doi: 10.1016/j.carpath.2013.01.003
25. [25]
  Y. Zhu, H. Tao, C. Jin, et al., Mol. Med. Rep. 12(2015) 5573-5579. doi: 10.3892/mmr.2015.4068
26. [26]
  K. Jin, Z.M. Luo, B. Zhang, et al., Acta Pharm. Sin. B 1(2018) 23-33.
27. [27]
  L. Montorfano, A. Becerra, R. Cerro, et al., Lab. Invest. 94(2014) 1068-1082. doi: 10.1038/labinvest.2014.100
1. [1]
  Shiqi Xu , Zi Ye , Shuang Shang , Fengge Wang , Huan Zhang , Lianguo Chen , Hao Lin , Chen Chen , Fang Hua , Chong-Jing Zhang . Pairs of thiol-substituted 1,2,4-triazole-based isomeric covalent inhibitors with tunable reactivity and selectivity. Chinese Chemical Letters, 2024, 35(7): 109034-. doi: 10.1016/j.cclet.2023.109034
2. [2]
  Xu-Hui Yue , Xiang-Wen Zhang , Hui-Min He , Lei Qiao , Zhong-Ming Sun . Synthesis, chemical bonding and reactivity of new medium-sized polyarsenides. Chinese Chemical Letters, 2024, 35(7): 108907-. doi: 10.1016/j.cclet.2023.108907
3. [3]
  Ying Li , Long-Jie Wang , Yong-Kang Zhou , Jun Liang , Bin Xiao , Ji-Shen Zheng . An improved installation of 2-hydroxy-4-methoxybenzyl (iHmb) method for chemical protein synthesis. Chinese Chemical Letters, 2024, 35(5): 109033-. doi: 10.1016/j.cclet.2023.109033
4. [4]
  Lian Sun , Honglei Wang , Ming Ma , Tingting Cao , Leilei Zhang , Xingui Zhou . Shape and composition evolution of Pt and Pt₃M nanocrystals under HCl chemical etching. Chinese Chemical Letters, 2024, 35(9): 109188-. doi: 10.1016/j.cclet.2023.109188
5. [5]
  Guangchang Yang , Shenglong Yang , Jinlian Yu , Yishun Xie , Chunlei Tan , Feiyan Lai , Qianqian Jin , Hongqiang Wang , Xiaohui Zhang . Regulating local chemical environment in O3-type layered sodium oxides by dual-site Mg²⁺/B³⁺ substitution achieves durable and high-rate cathode. Chinese Chemical Letters, 2024, 35(9): 109722-. doi: 10.1016/j.cclet.2024.109722
6. [6]
  Dake Liu , Shuyan Liu , Fanlei Hu , Zhongtang Li , Zhongjun Li . N-Glycosylated type Ⅱ collagen peptides as therapeutic saccharide vaccines for rheumatoid arthritis. Chinese Chemical Letters, 2024, 35(5): 108762-. doi: 10.1016/j.cclet.2023.108762
7. [7]
  Xueling Yu , Lixing Fu , Tong Wang , Zhixin Liu , Na Niu , Ligang Chen . Multivariate chemical analysis: From sensors to sensor arrays. Chinese Chemical Letters, 2024, 35(7): 109167-. doi: 10.1016/j.cclet.2023.109167
8. [8]
  Ruofan Yin , Zhaoxin Guo , Rui Liu , Xian-Sen Tao . Ultrafast synthesis of Na₃V₂(PO₄)₃ cathode for high performance sodium-ion batteries. Chinese Chemical Letters, 2025, 36(2): 109643-. doi: 10.1016/j.cclet.2024.109643
9. [9]
  Rong-Nan Yi , Wei-Min He . Photocatalytic Minisci-type multicomponent reaction for the synthesis of 1-(halo)alkyl-3-heteroaryl bicyclo[1.1.1]pentanes. Chinese Chemical Letters, 2024, 35(10): 110115-. doi: 10.1016/j.cclet.2024.110115
10. [10]
  Yuexiang Liu , Xiangqiao Yang , Tong Lin , Guantian Yang , Xiaoyong Xu , Bubing Zeng , Zhong Li , Weiping Zhu , Xuhong Qian . Efficient continuous synthesis of 2-[3-(trifluoromethyl)phenyl]malonic acid, a key intermediate of Triflumezopyrim, coupling with esterification-condensation-hydrolysis. Chinese Chemical Letters, 2025, 36(1): 109747-. doi: 10.1016/j.cclet.2024.109747
11. [11]
  Hong-Tao Ji , Yu-Han Lu , Yan-Ting Liu , Yu-Lin Huang , Jiang-Feng Tian , Feng Liu , Yan-Yan Zeng , Hai-Yan Yang , Yong-Hong Zhang , Wei-Min He . Nd@C₃N₄-photoredox/chlorine dual catalyzed synthesis and evaluation of antitumor activities of 4-alkylated sulfonyl ketimines. Chinese Chemical Letters, 2025, 36(2): 110568-. doi: 10.1016/j.cclet.2024.110568
12. [12]
  Min Fu , Pan He , Sen Zhou , Wenqiang Liu , Bo Ma , Shiying Shang , Yaohao Li , Ruihan Wang , Zhongping Tan . An unexpected stereochemical effect of thio-substituted Asp in native chemical ligation. Chinese Chemical Letters, 2024, 35(8): 109434-. doi: 10.1016/j.cclet.2023.109434
13. [13]
  Xianxu Chu , Lu Wang , Junru Li , Hui Xu . Surface chemical microenvironment engineering of catalysts by organic molecules for boosting electrocatalytic reaction. Chinese Chemical Letters, 2024, 35(8): 109105-. doi: 10.1016/j.cclet.2023.109105
14. [14]
  Ya-Nan Yang , Zi-Sheng Li , Sourav Mondal , Lei Qiao , Cui-Cui Wang , Wen-Juan Tian , Zhong-Ming Sun , John E. McGrady . Metal-metal bonds in Zintl clusters: Synthesis, structure and bonding in [Fe₂Sn₄Bi₈]^3– and [Cr₂Sb₁₂]^3–. Chinese Chemical Letters, 2024, 35(8): 109048-. doi: 10.1016/j.cclet.2023.109048
15. [15]
  Tao Zhou , Jing Zhou , Yunyun Liu , Jie-Ping Wan , Fen-Er Chen . Transition metal-free tunable synthesis of 3-(trifluoromethylthio) and 3-trifluoromethylsulfinyl chromones via domino C–H functionalization and chromone annulation of enaminones. Chinese Chemical Letters, 2024, 35(11): 109683-. doi: 10.1016/j.cclet.2024.109683
16. [16]
  Caili Yang , Tao Long , Ruotong Li , Chunyang Wu , Yuan-Li Ding . Pseudocapacitance dominated Li₃VO₄ encapsulated in N-doped graphene via 2D nanospace confined synthesis for superior lithium ion capacitors. Chinese Chemical Letters, 2025, 36(2): 109675-. doi: 10.1016/j.cclet.2024.109675
17. [17]
  Min Huang , Ru Cheng , Shuai Wen , Liangtong Li , Jie Gao , Xiaohui Zhao , Chunmei Li , Hongyan Zou , Jian Wang . Ultrasensitive detection of microRNA-21 in human serum based on the confinement effect enhanced chemical etching of gold nanorods. Chinese Chemical Letters, 2024, 35(9): 109379-. doi: 10.1016/j.cclet.2023.109379
18. [18]
  Yongjian Li , Xinyu Zhu , Chenxi Wei , Youyou Fang , Xinyu Wang , Yizhi Zhai , Wenlong Kang , Lai Chen , Duanyun Cao , Meng Wang , Yun Lu , Qing Huang , Yuefeng Su , Hong Yuan , Ning Li , Feng Wu . Unraveling the chemical and structural evolution of novel Li-rich layered/rocksalt intergrown cathode for Li-ion batteries. Chinese Chemical Letters, 2024, 35(12): 109536-. doi: 10.1016/j.cclet.2024.109536
19. [19]
  Xue Zheng , Jizhen Xie , Xing Zhang , Weiting Sun , Heyang Zhao , Yantuan Li , Cheng Wang . Corrigendum to "An overview of polymeric nanomicelles in clinical trials and on the market" [Chinese Chemical Letters 32 (2021) 243-257]. Chinese Chemical Letters, 2025, 36(2): 110545-. doi: 10.1016/j.cclet.2024.110545
20. [20]
  Huimin Luan , Qinming Wu , Jianping Wu , Xiangju Meng , Feng-Shou Xiao . Templates for the synthesis of zeolites. Chinese Journal of Structural Chemistry, 2024, 43(4): 100252-100252. doi: 10.1016/j.cjsc.2024.100252

Get Citation

PDF

XML

Metrics

PDF Downloads(7)
Abstract views(794)
HTML views(103)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142