Citation: Li Huang, Enben Su, Yuan Liu, Nongyue He, Yan Deng, Lian Jin, Zhu Chen, Song Li. A microfluidic device for accurate detection of hs-cTnI[J]. Chinese Chemical Letters, ;2021, 32(4): 1555-1558. doi: 10.1016/j.cclet.2020.09.055 shu

A microfluidic device for accurate detection of hs-cTnI

Li Huang ^{a, b} ,
Enben Su ^{a, b, *,} ,
Yuan Liu ^a ,
Nongyue He ^{a, c, *,} ,
Yan Deng ^{c, **,} ,
Lian Jin ^{a, c, *,} ,
Zhu Chen ^c ,
Song Li ^c

a.
State Key Laboratory of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
b.
Getein Biotechnology Co., Ltd., Nanjing 210000, China
c.
Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China

^**

Received Date: 19 September 2020
Revised Date: 30 September 2020
Accepted Date: 30 September 2020
Available Online: 1 October 2020

Figures(5)

This device is aimed at ensuring that the sample is uniformly and equivalently reacted with the antibody on the NC membrane in each test when the microfluidic liquid system is introduced to the chip. In this study, the developed microfluidic chip can avoid the presence of the sample and conjugate pads in the chip, while the precision of the chromatography system can be greatly improved using the same particles, NC membrane and antibody alongside the traditional strip. The results, taking the detection of cTnI as an example, revealed that the coefficient of variation (CV) is controlled within 4%, while the maximum record of the contrast chromatographic reagent strip can reach 15%. Additionally, the detection sensitivity can maintain the same order of magnitudes with that of the traditional chromatographic strip. With the results, the determination correlation of the developed microfluidic chip has been greatly improved. In addition, the CV of the chip in this study is greatly improved in comparison with that of the traditional strip. The biggest improvement lies in the mixing between the sample and the microspheres, indicating that this is a new approach to improve the CV of the traditional strip.
- Point of care testing,
- hs-cTnI,
- Microfluidic device,
- Precision
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