Citation: Shan He, Qitong Huang, Ying Zhang, Huifang Zhang, Huifeng Xu, Xun Li, Xiaoming Ma. Magnetic beads-based multicolor colorimetric immunoassay for ultrasensitive detection of aflatoxin B₁[J]. Chinese Chemical Letters, ;2021, 32(4): 1462-1465. doi: 10.1016/j.cclet.2020.09.047 shu

Magnetic beads-based multicolor colorimetric immunoassay for ultrasensitive detection of aflatoxin B₁

Shan He ^a ,
Qitong Huang ^b ,
Ying Zhang ^a ,
Huifang Zhang ^a ,
Huifeng Xu ^{c, **,} ,
Xun Li ^{a, **,} ,
Xiaoming Ma ^{a, d, *,}

a.
Department of Chemistry and Chemical Engineering, Gannan Normal University, Ganzhou 341000, China
b.
Scientific Research Center, Gannan Medical University, Ganzhou 341000, China
c.
Fujian Key Laboratory of Integrative Medicine on Geriatrics, Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350100, China
d.
State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, China

^**

xuhf@fjtcm.edu.cn

gnsylixun@163.com

maxlovery@163.com

Received Date: 27 June 2020
Revised Date: 4 August 2020
Accepted Date: 25 September 2020
Available Online: 28 September 2020

Figures(3)

Aflatoxin B₁ (AFB₁) is one of the most toxic, mutagenic and carcinogenic mycotoxin, widely exists in contaminated food, grains and feedstuff products. In this study, a novel magnetic beads multicolor colorimetric immunoassay (MBMCIA) based on Au@Ag nanorods (Au@Ag NRs) is proposed to visual detect ultralow concentration of AFB₁ with high-resolution by the naked-eye. To design the MBMCIA system, AFB₁-BSA conjugates were first coated on the surface of magnetic beads (MBs), then alkaline phosphatase (ALP) as a bridge between immunoassay and color reaction was used for catalytic hydrolysis of ascorbic acid-phosphate to generate reductive ascorbic acid. Finally, the yielded ascorbic acid could reduce silver ions to grow a silver coating on the surface of gold nanorods to generate Au@Ag NRs, which leads to the bule-shifted longitudinal absorption peak of Au NRs, accompanying with a series of perceptible color change. Under the optimal conditions, the proposed MBMCIA exhibited good sensitivity and specificity for the detection of AFB₁ with the detection limit as low as 5.7 pg/mL. Meanwhile, the MBMCIA was also applied for the analysis of AFB₁ in spiked wheat samples, the obtained recoveries range from 99.1% to 104.3% with relative standard deviation (RSD) less than 7.05% were acceptable. The proposed MBMCIA integrates separated, enriched, anti-interference and signal read-out into one, which opens up a new avenue for an on-site visual food safety inspection or environmental monitoring.
- Mycotoxin,
- Aflatoxin B₁,
- Magnetic beads,
- Immunoassay,
- Multicolor,
- Colorimetry
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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Magnetic beads-based multicolor colorimetric immunoassay for ultrasensitive detection of aflatoxin B1

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通讯作者: 陈斌, bchen63@163.com

Magnetic beads-based multicolor colorimetric immunoassay for ultrasensitive detection of aflatoxin B₁